#These sample sheet templates are to be used with Twist HT UDI (12bp) TruSeq compatible indexes for demultiplexing during FASTQ generation. Please read the whole README files before using. If an error occurs you can REQUEUE the analysis, no sequencing data will be lost.
 
#Templates available for all HT UDI plates combined in the 384 well and 98 well configurations.  Please delete any HT UDI index not used in your experiment run. If not deleted the index will show 0 reads/% assigned.

#These templates are for PAIRED END run configuration:
[Reads] needs match your run configuration 
Currently template set for 150x150
Sequencing kits vary in the number of additional cycles available for index reads. Use instrument manual guideline to ensure the total number of cycles for insert and index reads does not exceed the total cycles allowed by instrument and run configuration selected. 
If doing single end read, delete the row for the second read length
 
#Adapter trimming is turned ON in the template under [Settings]:
TruSeq adapter sequence for forward and reverse
If only sequencing in forward direction, delete the AdapterRead2 (and ReverseComplement field if applicable).
If you want to turn adapter trimming OFF, delete all the rows under and including [Settings]. The number of rows will differ depending on instrument. 
 
#File format:
Must be in .csv file extension. Please double check this after making any changes and saving.
 
#Sample sheet optional vs required fields:
 
Investigator Name - optional
Experiment Name - required 
Date (format) - X/XX/20XX (example: 4/23/2020)
Description - optional
Chemistry - optional
 
Sample_ID - required (must be unique)
Sample_Name - optional (only present on some instruments)
Sample_Plate - optional
Sample_Well - optional
Index_Plate_Well - optional (pre-filled)
I7_Index_ID - optional
Index - required (pre-filled)
I5_Index_ID - optional
Index2 - required (pre-filled)
Sample_Project - optional
Description - optional
 
#Sample sheets must be saved as a .csv file


