#These sample sheet templates are to be used with Twist UDI (10bp) TruSeq compatible indexes for demultiplexing during FASTQ generation. Please read the whole README files before using. If an error occurs you can REQUEUE the analysis, no sequencing data will be lost.
 
#Templates available for each UDI plate and all plates combined. Please delete any UDI not used in your experiment run. If not deleted the UDI will show 0 reads/% assigned.

#These templates are for PAIRED END run configuration:
[Reads] needs match your run configuration 
Currently template set for 76x76
If doing single end read, delete the row for the second read length 
 
#Adapter trimming is turned ON in the template under [Settings]:
TruSeq adapter sequence for forward and reverse
If only sequencing in forward direction, delete the AdapterRead2 (and ReverseComplement field if applicable).
If you want to turn adapter trimming OFF, delete all the rows under and including [Settings]. The number of rows will differ depending on instrument. 

#File naming format:
Company_IndexType_NumberSamples_PartNumber(if applicable)_Plate(s)_Instrument_i5Orientation(if applicable) 
 
#File format:
Must be in .csv file extension. Please double check this after making any changes and saving.
 
#Sample sheet optional vs required fields:
 
Investigator Name - optional
Experiment Name - required 
Date (format) - X/XX/20XX (example: 4/23/2020)
Description - optional
Chemistry - optional
 
Sample_ID - required (must be unique)
Sample_Name - optional (only present on some instruments)
Sample_Plate - optional
Sample_Well - optional
Index_Plate_Well - optional (pre-filled)
I7_Index_ID - optional
Index - required (pre-filled)
I5_Index_ID - optional
Index2 - required (pre-filled)
Sample_Project - optional
Description - optional
 
#Sample sheets must be saved as a .csv file


