Most comprehensive, up-to-date tool for interrogation
Most comprehensive, up-to-date tool for interrogation
Most comprehensive, up-to-date tool for interrogation
OVERVIEW DATA ORDERING & SPECS RESOURCES
OVERVIEW

The mouse is an extremely important model system for studying genetic variation, tumor mutations, and phenotypic outcomes as well as the therapeutic effect of pharmaceutical agents. As genetic variant databases are continuously updated, the Twist Mouse Exome panel is thoughtfully designed and built from the most current databases. When combined with Twist’s expanding portfolio of library preparation and enrichment reagents, the complete toolset allows researchers to achieve industry-leading coverage across target regions while optimizing sequencing cost and sample throughput.

BENEFITS
Up to date content
Based on mouse genome assembly mm10
37.7 Mb comprehensive coverage of major gene annotation databases
Unparalleled performance
Unparalleled performance
95% of targeted regions covered at 20x with only 5Gb sequencing
High complexity libraries for high sensitivity variant calling

The mouse is an extremely important model system for studying genetic variation, tumor mutations, and phenotypic outcomes as well as the therapeutic effect of pharmaceutical agents. As genetic variant databases are continuously updated, the Twist Mouse Exome panel is thoughtfully designed and built from the most current databases. When combined with Twist’s expanding portfolio of library preparation and enrichment reagents, the complete toolset allows researchers to achieve industry-leading coverage across target regions while optimizing sequencing cost and sample throughput.

BENEFITS
Up to date content
Based on mouse genome assembly mm10
37.7 Mb comprehensive coverage of major gene annotation databases
Unparalleled performance
Unparalleled performance
95% of targeted regions covered at 20x with only 5Gb sequencing
High complexity libraries for high sensitivity variant calling
DATA
Revolutionizing Target Enrichment

With 5-Gb sequencing, the narrow variance and mode of the coverage distribution (left) reduces both the number of bases found below the targeted level of coverage (right) and the over- and under-sequencing from the typically skewed coverage associated with poorer uniformity. This is aided by the balanced capture profile across a much wider range of target %GC content.

NGS Fixed Panel Mouse Exome Coverage Distribution and Percent Target Bases
Enabling genetic research of this important model organism

Picard metrics of the Twist Mouse Exome Kit with two strains of mouse. Data obtained with 5 Gb (135x) sequencing.

NGS Fixed Panel Mouse Exome Picard Metrics Table
Revolutionizing Target Enrichment

With 5-Gb sequencing, the narrow variance and mode of the coverage distribution (left) reduces both the number of bases found below the targeted level of coverage (right) and the over- and under-sequencing from the typically skewed coverage associated with poorer uniformity. This is aided by the balanced capture profile across a much wider range of target %GC content.

NGS Fixed Panel Mouse Exome Coverage Distribution and Percent Target Bases
Enabling genetic research of this important model organism

Picard metrics of the Twist Mouse Exome Kit with two strains of mouse. Data obtained with 5 Gb (135x) sequencing.

NGS Fixed Panel Mouse Exome Picard Metrics Table
ORDERING & SPECS
PRODUCT SKUS
102035 Twist Mouse Exome, 2 Reactions, Kit
102036 Twist Mouse Exome, 12 Reactions, Kit
102037 Twist Mouse Exome, 96 Reactions, Kit
SPECIFICATIONS
Workflow Step Target Enrichment
Format dsDNA
Panel size 37.7 Mb
Content Mouse protein coding genes
Design Databases RefSeq

The Twist Mouse Exome Panel is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

PRODUCT SKUS
102035 Twist Mouse Exome, 2 Reactions, Kit
102036 Twist Mouse Exome, 12 Reactions, Kit
102037 Twist Mouse Exome, 96 Reactions, Kit
SPECIFICATIONS
Workflow Step Target Enrichment
Format dsDNA
Panel size 37.7 Mb
Content Mouse protein coding genes
Design Databases RefSeq

The Twist Mouse Exome Panel is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

RESOURCES