开始定制您的基因
开始定制您的基因
开始定制您的基因
概述 克隆基因 基因片段 资源 参考文献
概述

克隆基因的新特性

我们升级了克隆基因产品,增加了质粒制备规模和其他选项(包括均一化、无内毒素 DNA 和各种试管和平板选项),以满足您的研究需求。

twist covid-19 antibodies

 

大规模、高质量

我们高精度的硅基 DNA 合成平台可生产更高质量的基因,并显著提高通量和可扩展性。



我们提供克隆基因和基因片段来满足您的研究需求:
克隆基因是克隆到质粒载体中并经 NGS 测序验证的合成 DNA。 

基因片段是合成的线性 DNA 序列,适用于直接克隆或较大的基因组装。


Twist 的基因产品能以您希望的方式灵活地为您提供所需的 DNA。扩展思路,扩大您的设计范围,加快研发。

 

Twist 基因在行动

了解科学家如何在流行病预防计划中使用 Twist 基因,该计划旨在加速抗体开发过程,其目标是在 60 天内获得治疗方法。

阅读案例研究

twist covid-19 antibodies

 

克隆基因的新特性

我们升级了克隆基因产品,增加了质粒制备规模和其他选项(包括均一化、无内毒素 DNA 和各种试管和平板选项),以满足您的研究需求。

twist covid-19 antibodies

 

大规模、高质量

我们高精度的硅基 DNA 合成平台可生产更高质量的基因,并显著提高通量和可扩展性。



我们提供克隆基因和基因片段来满足您的研究需求:
克隆基因是克隆到质粒载体中并经 NGS 测序验证的合成 DNA。 

基因片段是合成的线性 DNA 序列,适用于直接克隆或较大的基因组装。


Twist 的基因产品能以您希望的方式灵活地为您提供所需的 DNA。扩展思路,扩大您的设计范围,加快研发。

 

Twist 基因在行动

了解科学家如何在流行病预防计划中使用 Twist 基因,该计划旨在加速抗体开发过程,其目标是在 60 天内获得治疗方法。

阅读案例研究

twist covid-19 antibodies

 

克隆基因

克隆基因

Twist Bioscience 的平台每天能合成数千个基因来满足您的所有 DNA 需求。我们可扩展、高精度的硅基寡核苷酸生产可实现高质量的 DNA 合成和组装;这些品质让 Twist 能在您有需之时,将各种长度和难度,且经 NGS 验证后的完美基因及时地交付到您手中。

立即订购 or 获取产品说明书

 Low Cost – High Quality clonal gene
低成本 — 高质量
每个碱基 9 美分起
没有隐藏的亚克隆或 DNA 复杂度费用
最短交付时间为 11 个工作日
DNA Your Way clonal gene
DNA — 按您的方式
将 0.3 - 5 kb 基因克隆到您选择的质粒中
选择 Twist 目录载体或将您的载体寄给我们
4 种制备规模 (50 ng – 2 µg | 2 µg – 10 µg | 10 µg – 100 µg | 100 µg – 1 mg)
提供均一化化和无内毒素选项
Scalable Synthesis clonal gene
可扩展的合成
无最低订购数量限制
交付时间与订单大小无关
New Features for Clonal Genes

Get DNA your way with new prep sizes and options.

Get Started

Twist Maxi Preps

序列完美的基因

以下是我们对每个克隆基因进行标准 NGS 验证的图示。此图展示了没有错误的克隆基因。图中标出了整个质粒的测序深度,没有检测到 SNP 或插入缺失。

 

立即订购

序列完美的基因
克隆基因成功率

实测的 Twist Bioscience 克隆基因成功率。成功率定义为交付的克隆基因没有 SNP 或插入缺失的百分比。

立即订购

克隆基因成功率

克隆基因

Twist Bioscience 的平台每天能合成数千个基因来满足您的所有 DNA 需求。我们可扩展、高精度的硅基寡核苷酸生产可实现高质量的 DNA 合成和组装;这些品质让 Twist 能在您有需之时,将各种长度和难度,且经 NGS 验证后的完美基因及时地交付到您手中。

立即订购 or 获取产品说明书

New Features for Clonal Genes

Get DNA your way with new prep sizes and options.

Get Started

Twist Maxi Preps

 Low Cost – High Quality clonal gene
低成本 — 高质量
每个碱基 9 美分起
没有隐藏的亚克隆或 DNA 复杂度费用
最短交付时间为 11 个工作日
DNA Your Way clonal gene
DNA — 按您的方式
将 0.3 - 5 kb 基因克隆到您选择的质粒中
选择 Twist 目录载体或将您的载体寄给我们
4 种制备规模 (50 ng – 2 µg | 2 µg – 10 µg | 10 µg – 100 µg | 100 µg – 1 mg)
提供均一化化和无内毒素选项
Scalable Synthesis clonal gene
可扩展的合成
无最低订购数量限制
交付时间与订单大小无关
序列完美的基因

以下是我们对每个克隆基因进行标准 NGS 验证的图示。此图展示了没有错误的克隆基因。图中标出了整个质粒的测序深度,没有检测到 SNP 或插入缺失。

 

立即订购

序列完美的基因
克隆基因成功率

实测的 Twist Bioscience 克隆基因成功率。成功率定义为交付的克隆基因没有 SNP 或插入缺失的百分比。

立即订购

克隆基因成功率
基因片段

基因片段

Twist Bioscience 基因片段通过减少克隆筛选来改善克隆过程。大幅降低克隆和测序的成本,从而节省时间和金钱。思考更全面,突破规模限制,加速您的研究进展。

立即订购 or 获取产品说明书

Fast and Economical
快速、经济
每个碱基 7 美分
交付时间为 6 至 9 个工作日
基因片段可用于组装和克隆
兼容多种下游克隆方法
Screen Less Discover More Gene Fragments
减少筛选,拓展发现
平均错误率最低,1:3000
100 ng – 1 µg 的产量
300 bp – 1800 bp 的基因片段
可只订够 1 个基因片段

Best In Class Performance 

Twist’s DNA synthesis technology exhibits extremely low error rates. This level of precision makes larger gene assembly more reliable and easier to achieve. 

In a direct comparison with competitor products Twist outperformed with the lowest error rate. The same sequences were ordered from Twist and Integrated DNA Technologies, Inc. (IDT) for genes of various lengths, the genes were then cloned and transformed, colonies were picked and sequenced. The data represent the average error rate across the given sequences in each set. 

As seen in the figure below, Twist Gene Fragments had a nearly 2-fold improvement in error rate over IDT gBlocks across the entire size range and a 1.7-fold improvement over eBlocks across the range of 300 - 800 bp, with a 1:5,367 bp and 1:6,253 bp error rate respectively. Low error rates translate into fewer colonies that need to be picked and screened to find your perfect gene saving you time and money.
 

Gene Fragments average error rate


 

Affordable Gene Synthesis

Accurate fragment synthesis accelerates discovery. Twist Bioscience Gene Fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones. Twist’s lower overall error rate results in more perfect sequences. The performance and scale of Twists Gene synthesis translate into a lower price and less effort per gene. The following figure illustrates the cost per gene ordered as a Gene Fragment from Twist Bioscience. Buying ready-to-use genes is easier, faster, and less expensive than you thought.
 

Gene Fragments cost per gene

New Features for Gene Fragments

Gene Fragments now available with or without Twist Adapters.

Order Now

基因片段

Twist Bioscience 基因片段通过减少克隆筛选来改善克隆过程。大幅降低克隆和测序的成本,从而节省时间和金钱。思考更全面,突破规模限制,加速您的研究进展。

立即订购 or 获取产品说明书

Fast and Economical
快速、经济
每个碱基 7 美分
交付时间为 6 至 9 个工作日
基因片段可用于组装和克隆
兼容多种下游克隆方法
Screen Less Discover More Gene Fragments
减少筛选,拓展发现
平均错误率最低,1:3000
100 ng – 1 µg 的产量
300 bp – 1800 bp 的基因片段
可只订够 1 个基因片段

Best In Class Performance 

Twist’s DNA synthesis technology exhibits extremely low error rates. This level of precision makes larger gene assembly more reliable and easier to achieve. 

In a direct comparison with competitor products Twist outperformed with the lowest error rate. The same sequences were ordered from Twist and Integrated DNA Technologies, Inc. (IDT) for genes of various lengths, the genes were then cloned and transformed, colonies were picked and sequenced. The data represent the average error rate across the given sequences in each set. 

As seen in the figure below, Twist Gene Fragments had a nearly 2-fold improvement in error rate over IDT gBlocks across the entire size range and a 1.7-fold improvement over eBlocks across the range of 300 - 800 bp, with a 1:5,367 bp and 1:6,253 bp error rate respectively. Low error rates translate into fewer colonies that need to be picked and screened to find your perfect gene saving you time and money.
 

Gene Fragments average error rate


 

Affordable Gene Synthesis

Accurate fragment synthesis accelerates discovery. Twist Bioscience Gene Fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones. Twist’s lower overall error rate results in more perfect sequences. The performance and scale of Twists Gene synthesis translate into a lower price and less effort per gene. The following figure illustrates the cost per gene ordered as a Gene Fragment from Twist Bioscience. Buying ready-to-use genes is easier, faster, and less expensive than you thought.
 

Gene Fragments cost per gene

New Features for Gene Fragments

Gene Fragments now available with or without Twist Adapters.

Order Now

资源
参考文献
Testimonials
The combination of price and service allows us to do science that we could not otherwise do
PROFESSOR NICHOLAS HARMER
University of Exeter
The quality has been excellent, and I find that cloning with these genes is successful more often on the first time than with DNA fragments from other companies
DR. AMANDA PORTILLO
Institute of Systems and Synthetic Biology
This is where we came to really like the online ordering portal, that tells you then and there whether Twist can make your gene. We input our sequences and Twist’s algorithms right away said “yes, why not?”. It turned out that for Twist, making these difficult sequences was easy.
DR. PHILIPPE MARLIERE
Institute of Systems and Synthetic Biology

Genes Calculator

Gene Fragments*
Clonal Genes*
AVERAGE LENGTH

0.3kb

0.6kb

0.9kb

1.2kb

1.5kb

1.8kb
base pairs
Please enter a quantity between 300 and 1800.
QUANTITY
genes
Please enter a quantity between 1 and 10000.
ESTIMATED TOTAL
Based on cloning in a Twist vector
$54,000
TWIST CLONAL GENES WILL SAVE YOU AN ESTIMATED**
TWIST SAVES YOU AN ESTIMATED**
$1200on reagents & kits
BREAKDOWN
Vector prep
$45
Amplification
$35
Cloning
$60
QC
$40
TOTAL
~ $180 per gene
120 hrsof manual cloning
Manual cloning takes about 6 hours per 96 genes
ORDER NOW
SAVINGS WITH TWIST
COMPARED TO***
30%
SAVINGS
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.
**Cost savings based on comparison to public list prices for standard reagents. Time savings based on Twist's internal testing of typical times for standard manual cloning.
***Savings based on comparison to competitor’s publicly-listed pricing as of May 15, 2019. Turn-around times between Twist and competitors may vary.