Explore your protein’s sequence space
Explore your protein’s sequence space
Explore your protein’s sequence space
OVERVIEW DATA ORDERING AND SPECS RESOURCES
Overview

Generate 99% of Desired Variants

Protein engineering screens using single site variant libraries allow researchers to explore a protein’s sequence space and investigate the relationship between sequence and protein structure and function.


Twist Site Saturation Variant Library construction leverages massively parallel oligonucleotide synthesis using Twist's proprietary silicon-based DNA synthesis platform. Twist libraries are NGS-verified to confirm that all desired variants are present in the correct ratios.

Precisely Crafted Variant Libraries
Precisely Crafted Variant Libraries
Complete control over codon usage (all 64 codons available)
High uniformity of variant representation at every site
No unwanted codons or premature stop codons
Verified Quality
Verified Quality
Rigorous quality control
Verified uniformity of variant representation by NGS
Representation of each site normalized by mass
Flexibility
Flexibility
One position per well in a 96 well plate or all positions pooled in a single tube
Screen 1 to 20 different amino acids at each position
Experience base by base precision

Learn more about how Twist Single Site Variant Libraries (SSVL) enable the interrogation of sequence space to identify key residues in protein structure and function.

Watch the Webinar

Generate 99% of Desired Variants

Protein engineering screens using single site variant libraries allow researchers to explore a protein’s sequence space and investigate the relationship between sequence and protein structure and function.


Twist Site Saturation Variant Library construction leverages massively parallel oligonucleotide synthesis using Twist's proprietary silicon-based DNA synthesis platform. Twist libraries are NGS-verified to confirm that all desired variants are present in the correct ratios.

Precisely Crafted Variant Libraries
Precisely Crafted Variant Libraries
Complete control over codon usage (all 64 codons available)
High uniformity of variant representation at every site
No unwanted codons or premature stop codons
Verified Quality
Verified Quality
Rigorous quality control
Verified uniformity of variant representation by NGS
Representation of each site normalized by mass
Flexibility
Flexibility
One position per well in a 96 well plate or all positions pooled in a single tube
Screen 1 to 20 different amino acids at each position
Experience base by base precision

Learn more about how Twist Single Site Variant Libraries (SSVL) enable the interrogation of sequence space to identify key residues in protein structure and function.

Watch the Webinar

DATA
Explore the Sequence Space

Site Saturation Libraries eliminate codon bias and unwanted substitutions unlike traditional methods such as degenerate and NNK approaches. 

TRIM offers a poor repetitive yield, leading to <50% full-length product in a typical library whereas, Twist libraries yield more usable variants, increasing effective library size.

  Error Prone PCR Degenerate 

(NNK/NNS)

Twist Site Saturation
Variant Libraries
Eliminates sequence bias No No Yes
Number of codons available Unknown 32 All 64
Prevents undesirable motifs No No Yes
Allows codon optimization No No Yes
Avoids stop codons No Yes Yes

 

Highly Uniform Variant Libraries

Site Saturation Variant Libraries enable efficient sampling of a protein’s sequence space in screening assays.

This figure shows the observed distribution of amino acids across  65 positions in protein (19 intended variants per position) when built with Twist SSVLs versus error prone PCR. The bars represent a different amino acid position, with each color indicating the observed variant frequency. All variants are present in the expected ratios in the Twist Library.

SSVL Distribution

 

Explore the Sequence Space

Site Saturation Libraries eliminate codon bias and unwanted substitutions unlike traditional methods such as degenerate and NNK approaches. 

TRIM offers a poor repetitive yield, leading to <50% full-length product in a typical library whereas, Twist libraries yield more usable variants, increasing effective library size.

  Error Prone PCR Degenerate 

(NNK/NNS)

Twist Site Saturation
Variant Libraries
Eliminates sequence bias No No Yes
Number of codons available Unknown 32 All 64
Prevents undesirable motifs No No Yes
Allows codon optimization No No Yes
Avoids stop codons No Yes Yes

 

Highly Uniform Variant Libraries

Site Saturation Variant Libraries enable efficient sampling of a protein’s sequence space in screening assays.

This figure shows the observed distribution of amino acids across  65 positions in protein (19 intended variants per position) when built with Twist SSVLs versus error prone PCR. The bars represent a different amino acid position, with each color indicating the observed variant frequency. All variants are present in the expected ratios in the Twist Library.

SSVL Distribution

 

Ordering and Specs

We look forward to helping you place your order for your Site Saturation Variant Libraries. Please click on the "Get Quote" button above, where you will be directed to complete a short form and someone will be in contact with you shortly to help complete your order.

If you already have an account set up with Twist, you can log in and start completing your order details on-line.
 

We look forward to helping you place your order for your Site Saturation Variant Libraries. Please click on the "Get Quote" button above, where you will be directed to complete a short form and someone will be in contact with you shortly to help complete your order.

If you already have an account set up with Twist, you can log in and start completing your order details on-line.
 

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