More diversity, More discovery
More diversity, More discovery
More diversity, More discovery
OVERVIEW WORKFLOW ORDERING & SPECS RESOURCES
OVERVIEW

Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results. 

Available configurations

Mechanical Fragmentation

This kit is designed for use with gDNA that has already been sheared mechanically. It combines the enzymes and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.

  • Accommodates a wide range of DNA input types
  • Enables high-quality sequencing of low-quality samples
  • Minimizes start / stop site artifacts

Enzymatic Fragmentation

This kit incorporates the reagents and enzymes needed for DNA fragmentation with those for end repair, dA-tailing, and adapter ligation into a single reaction.

  • Ideal for automated, high-throughput library preparation
  • Produces tunable, reproducible fragment sizes
  • Minimizes sequence bias, maximizes coverage depth

 

Overview of workflow application options

Based on your application needs, create sequencing ready libraries in as little as two hours.
 

NGS Library Preparation Workflow

Mechanical Fragmentation
Accommodate Either Enzymatic or Mechanical Fragmentation
Use enzymatic fragmentation for automated, high-throughput gDNA
Use mechanical fragmentation for DNA input of varying types and quality
icon benefit sequencing
Accommodate Either Whole Genome Sequencing or Targeted Enrichment
Use with Twist full-length CD adapters for PCR-free library Preparation
Use with the Twist Universal Adapter (UDI) System for maximum yield
benefit efficient
Streamline Your Workflow
Optimized for use in the Twist Targeted NGS workflow
Flexible DNA sample input from 1 ng – 1 µg (enzymatic)
Convert DNA samples into robust, amplified libraries in under 2.5 hours
Combine library preparation steps into a single reaction for improved efficiency and consistent results

Twist Library Preparation Kits streamline the construction of high-quality DNA libraries for next-generation sequencing (NGS) applications. Two configurations accommodate either enzymatic or mechanical DNA fragmentation, and both are optimized to facilitate library preparation for whole genome sequencing and targeted enrichment. The kits combine library preparation steps into a single reaction for improved efficiency and consistent results. 

Available configurations

Mechanical Fragmentation

This kit is designed for use with gDNA that has already been sheared mechanically. It combines the enzymes and reagents for end repair, dA-tailing, and adapter ligation into a single reaction.

  • Accommodates a wide range of DNA input types
  • Enables high-quality sequencing of low-quality samples
  • Minimizes start / stop site artifacts

Enzymatic Fragmentation

This kit incorporates the reagents and enzymes needed for DNA fragmentation with those for end repair, dA-tailing, and adapter ligation into a single reaction.

  • Ideal for automated, high-throughput library preparation
  • Produces tunable, reproducible fragment sizes
  • Minimizes sequence bias, maximizes coverage depth

 

Overview of workflow application options

Based on your application needs, create sequencing ready libraries in as little as two hours.
 

NGS Library Preparation Workflow

Mechanical Fragmentation
Accommodate Either Enzymatic or Mechanical Fragmentation
Use enzymatic fragmentation for automated, high-throughput gDNA
Use mechanical fragmentation for DNA input of varying types and quality
icon benefit sequencing
Accommodate Either Whole Genome Sequencing or Targeted Enrichment
Use with Twist full-length CD adapters for PCR-free library Preparation
Use with the Twist Universal Adapter (UDI) System for maximum yield
benefit efficient
Streamline Your Workflow
Optimized for use in the Twist Targeted NGS workflow
Flexible DNA sample input from 1 ng – 1 µg (enzymatic)
Convert DNA samples into robust, amplified libraries in under 2.5 hours
Combine library preparation steps into a single reaction for improved efficiency and consistent results
WORKFLOW
Overview of the library preparation workflow

Both Twist Library Preparation Kits include the reagents required for end repair, dA-tailing, and adapter ligation. The Enzymatic Fragmentation Kit also incorporates the enzymes for fragmentation of gDNA samples. Following core library construction, either full length or universal adapters can be used to suit your application needs.


The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.

 

library preparation workflow with Twist Universal Adapters.
Overview of the library preparation workflow

Both Twist Library Preparation Kits include the reagents required for end repair, dA-tailing, and adapter ligation. The Enzymatic Fragmentation Kit also incorporates the enzymes for fragmentation of gDNA samples. Following core library construction, either full length or universal adapters can be used to suit your application needs.


The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.

 

library preparation workflow with Twist Universal Adapters.
ORDERING & SPECS
PRODUCT SKUS – ENZYMATIC FRAGMENTATION
101059 Twist Library Preparation EF Kit, Enzymatic Fragmentation, 16 Samples
101058 Twist Library Preparation EF Kit, Enzymatic Fragmentation, 96 Samples Reagents, enzymes, and purification beads required for enzymatic fragmentation of gDNA and library construction
PRODUCT SKUS – MECHANICAL FRAGMENTATION
101280 Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples
101281 Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples Reagents, enzymes, and purification beads required for the library construction of mechanically fragmented gDNA

The Twist NGS Library Preparation Kits are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

PRODUCT SKUS – ENZYMATIC FRAGMENTATION
101059 Twist Library Preparation EF Kit, Enzymatic Fragmentation, 16 Samples
101058 Twist Library Preparation EF Kit, Enzymatic Fragmentation, 96 Samples Reagents, enzymes, and purification beads required for enzymatic fragmentation of gDNA and library construction
PRODUCT SKUS – MECHANICAL FRAGMENTATION
101280 Twist Library Preparation Kit, Mechanical Fragmentation, 16 Samples
101281 Twist Library Preparation Kit, Mechanical Fragmentation, 96 Samples Reagents, enzymes, and purification beads required for the library construction of mechanically fragmented gDNA

The Twist NGS Library Preparation Kits are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

RESOURCES