Reproducible. Accurate. Safe.
Reproducible. Accurate. Safe.
Reproducible. Accurate. Safe.
OVERVIEW SARS-COV-2 CONTROLS RESPIRATORY VIRUS CONTROLS ORDERING AND SPECS RESOURCES
Overview

Trusted, accurate and safe controls for
a wide-range of applications

The recent Coronavirus pandemic has led to the unprecedented need for diagnostic tests for detecting the presence of SARS-CoV-2 and other Respiratory viruses in a variety of sample types. To address this need, laboratories around the world need high quality tools to enable them in catering to this rapidly expanding requirement for testing. 

 

Reproducible, Quality Results

Positive controls provide quality control measures for a wide range of applications from diagnostic assay development to day-to-day testing. This includes the verification and validation of diagnostic tests of both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays. 

 

A Safe and Effective Alternative to “Live Virus”

Controls based on viral nucleic acids extracted from either an infected patient or from live virus propagated in cell culture have safety and security concerns. Synthetic controls created through gene synthesis broaden access across diverse strains while mitigating risk.

 

Flexible formats for your applications

Choose from Standard Control or Assay Ready formulations. Standard provides the controls at high concentration in a frozen liquid formulation, while Assay Ready Controls are provided as a dried pellet that increases stability. The enhanced stability of the Assay Ready format allows for shipping at ambient temperatures and storage in standard freezers saving valuable space in ultra-low freezers. Ambient shipping reduces cost and increases accessibility. 


 

  Standard Controls Assay Ready Controls
Biosafety Level 1 Level 1
Storage Temperature: -70°C to -90°C Temperature: -20°C
Specification Range Approximately 1x10^6 copies/μL Approximately 2x10^6 copies/tube
Physical State Frozen liquid  Dried pellet
Sensitive Detection
Fully synthetic RNA
Includes fully synthetic RNA generated from Twist gene fragments
Powerful alternative to live viruses
Sequencing
Full viral genome coverage
>99.9% viral genome coverage
NGS sequence verified
Enables versatility in assay design
Versatile controls
Versatile controls
Positive controls perform for both qPCR and NGS assays
Content is well-aligned with Twist target enrichment panels
New Controls to Cover UK Variant

Two new controls have been added that include the new variant strain of SARS-CoV-2 (VoC-202012/01, B.1.1.7 lineage) identified in the United Kingdom.
This new strain contains multiple mutations in the spike protein and other areas of the viral genome.
Some RT-PCR probes used widely in SARS-CoV-2 testing detect the S gene that codes for the spike protein.
Our new controls will help you avoid false negative results when screening the new strain.

TARGETED TOOLS FOR SARS-CoV-2 RESEARCH

Hear how these new products are making a difference in testing, surveillance, vaccine development and therapeutic antibody discoveries.

Watch the Webinar

Trusted, accurate and safe controls for
a wide-range of applications

The recent Coronavirus pandemic has led to the unprecedented need for diagnostic tests for detecting the presence of SARS-CoV-2 and other Respiratory viruses in a variety of sample types. To address this need, laboratories around the world need high quality tools to enable them in catering to this rapidly expanding requirement for testing. 

 

Reproducible, Quality Results

Positive controls provide quality control measures for a wide range of applications from diagnostic assay development to day-to-day testing. This includes the verification and validation of diagnostic tests of both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays. 

 

A Safe and Effective Alternative to “Live Virus”

Controls based on viral nucleic acids extracted from either an infected patient or from live virus propagated in cell culture have safety and security concerns. Synthetic controls created through gene synthesis broaden access across diverse strains while mitigating risk.

 

Flexible formats for your applications

Choose from Standard Control or Assay Ready formulations. Standard provides the controls at high concentration in a frozen liquid formulation, while Assay Ready Controls are provided as a dried pellet that increases stability. The enhanced stability of the Assay Ready format allows for shipping at ambient temperatures and storage in standard freezers saving valuable space in ultra-low freezers. Ambient shipping reduces cost and increases accessibility. 


 

  Standard Controls Assay Ready Controls
Biosafety Level 1 Level 1
Storage Temperature: -70°C to -90°C Temperature: -20°C
Specification Range Approximately 1x10^6 copies/μL Approximately 2x10^6 copies/tube
Physical State Frozen liquid  Dried pellet
Sensitive Detection
Fully synthetic RNA
Includes fully synthetic RNA generated from Twist gene fragments
Powerful alternative to live viruses
Sequencing
Full viral genome coverage
>99.9% viral genome coverage
NGS sequence verified
Enables versatility in assay design
Versatile controls
Versatile controls
Positive controls perform for both qPCR and NGS assays
Content is well-aligned with Twist target enrichment panels
New Controls to Cover UK Variant

Two new controls have been added that include the new variant strain of SARS-CoV-2 (VoC-202012/01, B.1.1.7 lineage) identified in the United Kingdom.
This new strain contains multiple mutations in the spike protein and other areas of the viral genome.
Some RT-PCR probes used widely in SARS-CoV-2 testing detect the S gene that codes for the spike protein.
Our new controls will help you avoid false negative results when screening the new strain.

TARGETED TOOLS FOR SARS-CoV-2 RESEARCH

Hear how these new products are making a difference in testing, surveillance, vaccine development and therapeutic antibody discoveries.

Watch the Webinar

SARS-CoV-2 Controls
The use of positive controls in quality control

Twist has created synthetic RNA controls for several viral genomes. These variants were selected to cover a wide range of sequence diversity. Each control consists of six non-overlapping 5 kb fragments generated from Twist Gene Fragments then transcribed into ssRNA. These controls provide coverage of greater than 99.9% of the bases of the SARS-CoV-2 viral genome. The majority of the controls are provided in the standard format which consist of 100 μL at a concentration of approximately one million copies per microliter. Twist also offers certain controls in an Assay Ready format. Assay Ready Controls are supplied as a desiccated pellet that includes a nucleic acid stabilizer which enables greater stability and ease of use during shipping and storage. The Assay Ready Controls are supplied at approximately two million copies per tube. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control was developed in partnership with the US CDC and integrated into the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay, which tests tests for influenza A, B and SARS-CoV-2 simultaneously. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control is now available for customers outside the CDC. 

NGS SARS-CoV-2 Synthetic RNA Controls Genome
Available SARS-CoV-2 Controls

GenBank IDs and GISAID names for synthetic controls 1 through 15.

As the pandemic continues to evolve and as new strains emerge, we’ll continue to expand this panel of synthetic controls to ensure you have the most up-to-date tools to track viral progression and evolution.

Twist Bioscience Assay Ready Control products are dried down and shipped at room temperature.  Although the controls are stable under most standard laboratory storage conditions, it is important to consider the following best practices to maintain the highest possible quality and prevent degradation. 

 

Best Practices for Working with Twist Assay Ready Controls
 

  • Assay Ready Controls are shipped at ambient temperature and should be stored at -20°C upon receipt. 
  • The controls are intended for one time use only. 
  • RNA controls should be kept on ice while in use as RNA is easily degraded.  
  • Use only RNase free consumables when handling this product. 
  • Before using, briefly centrifuge the tube to pellet RNA at the bottom on the tube and resuspend in RNase-free Water. 
  • A resuspension volume of 50 - 200 µL is recommended to generate a final viral concentration of approximately 40,000 - 10,000 copies per µLrespectively.
  • Gently pipette mix or vortex to resuspend.
  • Note: the stable dried down format includes Tris-EDTA (TE) buffer at pH 8.0. Adding additional TE could impact downstream assays. 
     
PART NUMBER CONTROL GENBANK ID GISAID NAME
102019 Control 1 MT007544.1 Australia/VIC01/2020
102024 Control 2 MN908947.3 Wuhan-Hu-1
103925 Assay Ready Control 2 MN908947.3 Wuhan-Hu-1
102860 Control 3 LC528232.1 Japan/Hu_DP_Kng_19-020/2020
102862 Control 4 MT106054.1 USA/TX1/2020
102917 Control 5 MT188340 USA/MN2-MDH2/2020
102918 Control 6 MT118835 USA/CA9/2020
103087 Control 7 EPI_ISL_418227 France/HF2393/2020
103511 Control 8 MT066176 Taiwan/NTU02/2020
103512 Control 9 MT152824 USA/WA2/2020
103513 Control 10 EPI_ISL_414648 USA/CA-PC101P/2020
103514 Control 11 EPI_ISL_417739 Iceland/5/2020
103515 Control 12 EPI_ISL_420244 England/SHEF-C05B2/2020
103533 Control 13 EPI_ISL_421184 Belgium/ULG-10004/2020
103907 Control 14 EPI_ISL_710528 England/205041766/2020
103909 Control 15 EPI_ISL_601443 England/MILK-9E05B3/2020

 

The use of positive controls in quality control

Twist has created synthetic RNA controls for several viral genomes. These variants were selected to cover a wide range of sequence diversity. Each control consists of six non-overlapping 5 kb fragments generated from Twist Gene Fragments then transcribed into ssRNA. These controls provide coverage of greater than 99.9% of the bases of the SARS-CoV-2 viral genome. The majority of the controls are provided in the standard format which consist of 100 μL at a concentration of approximately one million copies per microliter. Twist also offers certain controls in an Assay Ready format. Assay Ready Controls are supplied as a desiccated pellet that includes a nucleic acid stabilizer which enables greater stability and ease of use during shipping and storage. The Assay Ready Controls are supplied at approximately two million copies per tube. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control was developed in partnership with the US CDC and integrated into the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay, which tests tests for influenza A, B and SARS-CoV-2 simultaneously. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control is now available for customers outside the CDC. 

NGS SARS-CoV-2 Synthetic RNA Controls Genome
Available SARS-CoV-2 Controls

GenBank IDs and GISAID names for synthetic controls 1 through 15.

As the pandemic continues to evolve and as new strains emerge, we’ll continue to expand this panel of synthetic controls to ensure you have the most up-to-date tools to track viral progression and evolution.

Twist Bioscience Assay Ready Control products are dried down and shipped at room temperature.  Although the controls are stable under most standard laboratory storage conditions, it is important to consider the following best practices to maintain the highest possible quality and prevent degradation. 

 

Best Practices for Working with Twist Assay Ready Controls
 

  • Assay Ready Controls are shipped at ambient temperature and should be stored at -20°C upon receipt. 
  • The controls are intended for one time use only. 
  • RNA controls should be kept on ice while in use as RNA is easily degraded.  
  • Use only RNase free consumables when handling this product. 
  • Before using, briefly centrifuge the tube to pellet RNA at the bottom on the tube and resuspend in RNase-free Water. 
  • A resuspension volume of 50 - 200 µL is recommended to generate a final viral concentration of approximately 40,000 - 10,000 copies per µLrespectively.
  • Gently pipette mix or vortex to resuspend.
  • Note: the stable dried down format includes Tris-EDTA (TE) buffer at pH 8.0. Adding additional TE could impact downstream assays. 
     
PART NUMBER CONTROL GENBANK ID GISAID NAME
102019 Control 1 MT007544.1 Australia/VIC01/2020
102024 Control 2 MN908947.3 Wuhan-Hu-1
103925 Assay Ready Control 2 MN908947.3 Wuhan-Hu-1
102860 Control 3 LC528232.1 Japan/Hu_DP_Kng_19-020/2020
102862 Control 4 MT106054.1 USA/TX1/2020
102917 Control 5 MT188340 USA/MN2-MDH2/2020
102918 Control 6 MT118835 USA/CA9/2020
103087 Control 7 EPI_ISL_418227 France/HF2393/2020
103511 Control 8 MT066176 Taiwan/NTU02/2020
103512 Control 9 MT152824 USA/WA2/2020
103513 Control 10 EPI_ISL_414648 USA/CA-PC101P/2020
103514 Control 11 EPI_ISL_417739 Iceland/5/2020
103515 Control 12 EPI_ISL_420244 England/SHEF-C05B2/2020
103533 Control 13 EPI_ISL_421184 Belgium/ULG-10004/2020
103907 Control 14 EPI_ISL_710528 England/205041766/2020
103909 Control 15 EPI_ISL_601443 England/MILK-9E05B3/2020

 

Respiratory Virus Controls
Available Respiratory Virus Controls

The Twist Synthetic Respiratory Virus Controls cover a broad range of RNA and DNA viruses relevant to respiratory disease research. These controls are aligned with the content of the Twist Respiratory Virus Research Panel (PN 103067) and are suitable for use with Twist Fixed Panel NGS workflows or for use with RT-PCR or qPCR experiments of your own design. 

The figure shows a taxonomic tree of viruses covered by the Twist Respiratory Virus Controls. The table shows GenBank IDs, virus type and length for each control. Additional viral controls may be available for custom synthesis. Please contact your local sales representative for more details.
 

NGS Respiratory Viral Controls Taxonomic Tree
Virus name Genbank IDs, virus type, and lengths

Virus name Genbank IDs, virus type, and length for each virus included in the Twist Respiratory Virus Controls. Additional viral controls may be available for custom synthesis.

Contact your local sales representative for more details or Buy Now.

NGS Respiratory Viral Controls Part Numbers
Available Respiratory Virus Controls

The Twist Synthetic Respiratory Virus Controls cover a broad range of RNA and DNA viruses relevant to respiratory disease research. These controls are aligned with the content of the Twist Respiratory Virus Research Panel (PN 103067) and are suitable for use with Twist Fixed Panel NGS workflows or for use with RT-PCR or qPCR experiments of your own design. 

The figure shows a taxonomic tree of viruses covered by the Twist Respiratory Virus Controls. The table shows GenBank IDs, virus type and length for each control. Additional viral controls may be available for custom synthesis. Please contact your local sales representative for more details.
 

NGS Respiratory Viral Controls Taxonomic Tree
Virus name Genbank IDs, virus type, and lengths

Virus name Genbank IDs, virus type, and length for each virus included in the Twist Respiratory Virus Controls. Additional viral controls may be available for custom synthesis.

Contact your local sales representative for more details or Buy Now.

NGS Respiratory Viral Controls Part Numbers
Ordering and Specs
PRODUCT SKUS – SARS-COV-2 SYNTHETIC CONTROLS
102019 Control 1 (MT007544.1)
102024 Control 2 (MN908947.3)
103925 Assay Ready Control 2 (MN908947.3)
102860 Control 3 (LC528232.1)
102862 Control 4 (MT106054.1)
102917 Control 5 (MT188340)
102918 Control 6 (MT118835)
103087 Control 7 (EPI_ISL_418227)
103086 Controls (1–7)
103511 Control 8 (MT066176)
103512 Control 9 (MT152824)
103513 Control 10 (EPI_ISL_414648)
103514 Control 11 (EPI_ISL_417739)
103515 Control 12 (EPI_ISL_420244)
103533 Control 13 (EPI_ISL_421184)
103907 Control 14 (B.1.1.7_710528)
103909 Control 15 (B.1.1.7_601443)
PRODUCT SKUS – RESPIRATORY VIRUS CONTROLS
103001 Twist Synthetic Influenza H1N1 (2009) RNA control - NC_026431, NC_026432, NC_026433, NC_026434, NC_026435, NC_026436, NC_026437, NC_026438
103002 Twist Synthetic Influenza H3N2 RNA control - NC_007366, NC_007367, NC_007368, NC_007369, NC_007370, NC_007371, NC_007372, NC_007373
103003 Twist Synthetic Influenza B RNA control - NC_002204, NC_002205, NC_002206, NC_002207, NC_002208, NC_002209, NC_002210, NC_002211
103004 Twist Synthetic Human bocavirus 1 DNA control - MG953830.1
103005 Twist Synthetic Human enterovirus 68 RNA control - NC_038308.1
103006 Twist Synthetic Human rhinovirus 89 RNA control - NC_001617.1
103007 Twist Synthetic Mumps virus RNA control - NC_002200.1
103008 Twist Synthetic Human parainfluenza virus 1 RNA control - NC_003461.1
103009 Twist Synthetic Measles virus RNA control - NC_001498.1
103010 Twist Synthetic Human parainfluenza virus 4 RNA control - NC_021928.1
103011 Twist Synthetic Human coronavirus 229E RNA control - NC_002645.1
103012 Twist Synthetic Human coronavirus NL63 RNA control - NC_005831.2
103013 Twist Synthetic Human coronavirus OC43 RNA control - NC_006213.1
103730 Twist Respiratory Virus Controls
SPECIFICATIONS
Biosafety Level 1
Storage Temperature: -70°C to -90°C
Specification Range Approximately 1x10^6 copies/μL

The Twist Synthetic Viral Controls are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

PRODUCT SKUS – SARS-COV-2 SYNTHETIC CONTROLS
102019 Control 1 (MT007544.1)
102024 Control 2 (MN908947.3)
103925 Assay Ready Control 2 (MN908947.3)
102860 Control 3 (LC528232.1)
102862 Control 4 (MT106054.1)
102917 Control 5 (MT188340)
102918 Control 6 (MT118835)
103087 Control 7 (EPI_ISL_418227)
103086 Controls (1–7)
103511 Control 8 (MT066176)
103512 Control 9 (MT152824)
103513 Control 10 (EPI_ISL_414648)
103514 Control 11 (EPI_ISL_417739)
103515 Control 12 (EPI_ISL_420244)
103533 Control 13 (EPI_ISL_421184)
103907 Control 14 (B.1.1.7_710528)
103909 Control 15 (B.1.1.7_601443)
PRODUCT SKUS – RESPIRATORY VIRUS CONTROLS
103001 Twist Synthetic Influenza H1N1 (2009) RNA control - NC_026431, NC_026432, NC_026433, NC_026434, NC_026435, NC_026436, NC_026437, NC_026438
103002 Twist Synthetic Influenza H3N2 RNA control - NC_007366, NC_007367, NC_007368, NC_007369, NC_007370, NC_007371, NC_007372, NC_007373
103003 Twist Synthetic Influenza B RNA control - NC_002204, NC_002205, NC_002206, NC_002207, NC_002208, NC_002209, NC_002210, NC_002211
103004 Twist Synthetic Human bocavirus 1 DNA control - MG953830.1
103005 Twist Synthetic Human enterovirus 68 RNA control - NC_038308.1
103006 Twist Synthetic Human rhinovirus 89 RNA control - NC_001617.1
103007 Twist Synthetic Mumps virus RNA control - NC_002200.1
103008 Twist Synthetic Human parainfluenza virus 1 RNA control - NC_003461.1
103009 Twist Synthetic Measles virus RNA control - NC_001498.1
103010 Twist Synthetic Human parainfluenza virus 4 RNA control - NC_021928.1
103011 Twist Synthetic Human coronavirus 229E RNA control - NC_002645.1
103012 Twist Synthetic Human coronavirus NL63 RNA control - NC_005831.2
103013 Twist Synthetic Human coronavirus OC43 RNA control - NC_006213.1
103730 Twist Respiratory Virus Controls
SPECIFICATIONS
Biosafety Level 1
Storage Temperature: -70°C to -90°C
Specification Range Approximately 1x10^6 copies/μL

The Twist Synthetic Viral Controls are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

RESOURCES