Exceptional performance at any Scale
Exceptional performance at any Scale
Exceptional performance at any Scale
OVERVIEW DATA APPLICATIONS SPECS RESOURCES
Overview

Performance at any scale

Our revolutionary silicon-based DNA Synthesis platform is capable of producing over a million unique ssDNA oligos in a single run. Each chip contains Thousands of discrete clusters each containing 121 individually addressable surfaces capable of synthesizing a unique oligo sequence. A representation of the silicon chip technology can be seen below.

Due to the sheer scale of DNA synthesis capability on the chip there are virtually no limitations to the number of unique oligos that can be assembled into a pool. Don't restrict the outcome of your application, order Oligo Pools tailored precisely to your experiment. 

Performance at any scale

 

More Space for Innovation
 

Don’t be limited by length. Encode the elements and sequences you need, including promoters, enhancers, guides, and probes all within the expanded architecture. With oligo lengths up to 300nt it’s possible to encode multiple elements in a single design giving you the ability to do more with each oligo.

 

More Space for Innovation

 


Screen once, screen right
 

Unmatched pool uniformity without bias provides more unique targets per screen. This means less effort is required to achieve the data coverage you need, saving time and money.

Screen once screen right

 


Be data confident
 

With the precision and uniformity of Twist Oligo Pools, you can be confident in the quality of your experimental results. Don’t miss a datapoint due to synthesis errors or inefficiencies. Get industry-leading oligo representation and low error rate for oligos of any length up to 300nt, and pools of any size. Leverage these exceptional quality oligo pools at the precise scale and length required for your application.

 

Data Confident

GET THE APP NOTE

Learn how you can create multiple focused CRISPR libraries from a single Oligo Pool to expand your research and save time and money in the process.

Multiple focused CRISPR libraries from a single Oligo Pool

Performance at any scale

Our revolutionary silicon-based DNA Synthesis platform is capable of producing over a million unique ssDNA oligos in a single run. Each chip contains Thousands of discrete clusters each containing 121 individually addressable surfaces capable of synthesizing a unique oligo sequence. A representation of the silicon chip technology can be seen below.

Due to the sheer scale of DNA synthesis capability on the chip there are virtually no limitations to the number of unique oligos that can be assembled into a pool. Don't restrict the outcome of your application, order Oligo Pools tailored precisely to your experiment. 

Performance at any scale

 

More Space for Innovation
 

Don’t be limited by length. Encode the elements and sequences you need, including promoters, enhancers, guides, and probes all within the expanded architecture. With oligo lengths up to 300nt it’s possible to encode multiple elements in a single design giving you the ability to do more with each oligo.

 

More Space for Innovation

 


Screen once, screen right
 

Unmatched pool uniformity without bias provides more unique targets per screen. This means less effort is required to achieve the data coverage you need, saving time and money.

Screen once screen right

 


Be data confident
 

With the precision and uniformity of Twist Oligo Pools, you can be confident in the quality of your experimental results. Don’t miss a datapoint due to synthesis errors or inefficiencies. Get industry-leading oligo representation and low error rate for oligos of any length up to 300nt, and pools of any size. Leverage these exceptional quality oligo pools at the precise scale and length required for your application.

 

Data Confident

GET THE APP NOTE

Learn how you can create multiple focused CRISPR libraries from a single Oligo Pool to expand your research and save time and money in the process.

Multiple focused CRISPR libraries from a single Oligo Pool

Data
Highly Uniform Oligo Synthesis

NGS quality control data from a typical oligo pool containing 23,000 90mer oligos show the uniformity of the pool at 300x read coverage. The corresponding table indicates the uniformity metrics for this pool. Twist oligos are synthesized bias-free with high uniformity and complete oligo representation.

 

Oligo Pools Read Counts Histogram Uniformity
Sequencing Analysis of Oligo Pools

Sequencing analysis of Oligo Pools generated by Twist Bioscience and an array-based competitor demonstrate that the Twist Oligo Pools contain 100% of the expected sequences and a higher percentage of correct sequences than the competitor pool.

Oligo Pools Sequencing Analysis
Highly Uniform Oligo Synthesis

NGS quality control data from a typical oligo pool containing 23,000 90mer oligos show the uniformity of the pool at 300x read coverage. The corresponding table indicates the uniformity metrics for this pool. Twist oligos are synthesized bias-free with high uniformity and complete oligo representation.

 

Oligo Pools Read Counts Histogram Uniformity
Sequencing Analysis of Oligo Pools

Sequencing analysis of Oligo Pools generated by Twist Bioscience and an array-based competitor demonstrate that the Twist Oligo Pools contain 100% of the expected sequences and a higher percentage of correct sequences than the competitor pool.

Oligo Pools Sequencing Analysis
Applications

​​​​​​Guide RNA libraries for CRISPR screens

CRISPR screens involve the precise, high-throughput knock-out, interference or modulation of gene activity, allowing complex genotype-phenotype interactions to be unveiled. Oligo Pools are designed to encode multiple guide RNA molecules per gene.  

Product benefits include:

  • Full control over guide library design, avoiding unwanted guides, wasted sequencing, and limited guide-per-gene ratios.
  • Pairing high-fidelity Cas enzymes with highly accurate synthesis for true high-fidelity screens with minimal off-target effects.
  • Incredibly uniform, bias-free pools achieve amplified signal to noise ratio to achieve maximum hit detection, lowering downstream costs.
  • Oligos up to 300nt make space for additional experimental elements. Add multiple guides per oligo, genome homology, or minimal promoters with ease.

Next generation CRISPR screens based on single cell transcriptomics

Watch our webinar: Next generation CRISPR screens based on single-cell transcriptomics

Genome tiling libraries

Tiling libraries allow a comprehensive study of genomic functionality. Pools of oligos may encode peptide arrays, mRNA encoding sequences, or genome fragments.

Product benefits include:

  • Tiling precise genomic regions of interest. Reduce experimental noise by ignoring unwanted sequences at the design step.
  • Complex, repetitive or palindromic sequences that cannot typically be synthesized as genes can be encoded in oligos.
  • Larger peptide structures or genome architectures can be encoded in oligos 300nt in length.
  • Uniform synthesis minimizes oversampling required for comprehensive screening.

Identifying Coronavirus Virulence Factors for RNA Vaccine Development

Read our article: Identifying Coronavirus Virulence Factors for RNA Vaccine Development with Twist Oligo Pools

High throughput genetic control screens

Understanding the genetic elements controlling gene expression and regulation has broad application in synthetic biology and development of potential disease treatment. Oligo Pools power high-throughput screens of genetic control, including gene expression profiling and reporter-based applications. 

Product benefits include:

  • High-quality pools ensure you can interrogate every base for functionality
  • High uniformity means you screen less to find your answers
  • Up to 300nt pools gives you the flexibility to add multiple elements or homology regions

Epitranscriptome Screening on TechNetworks

Watch our webinar: Epitranscriptome Screening on TechNetworks

Fluorescence in-situ hybridization

Fluorescence in-situ hybridization (FISH) allows the abundance and spatial organization of nucleic acids in biological samples to be directly visualized. Oligo Pools can encode probes that when bound to fluorescent markers provide tunable, programmable visualization of large numbers of targets.

Product benefits include:

  • Rapid FISH probe development
  • Sequence control enables effective targeting
  • Longer oligos enable more specific targeting

Rapid and efficient production of FISH probes using oligo pools with Nicola Crosetto

Watch our webinar: Rapid and efficient production of FISH probes using oligo pools with Nicola Crosetto

​​​​​​Guide RNA libraries for CRISPR screens

CRISPR screens involve the precise, high-throughput knock-out, interference or modulation of gene activity, allowing complex genotype-phenotype interactions to be unveiled. Oligo Pools are designed to encode multiple guide RNA molecules per gene.  

Product benefits include:

  • Full control over guide library design, avoiding unwanted guides, wasted sequencing, and limited guide-per-gene ratios.
  • Pairing high-fidelity Cas enzymes with highly accurate synthesis for true high-fidelity screens with minimal off-target effects.
  • Incredibly uniform, bias-free pools achieve amplified signal to noise ratio to achieve maximum hit detection, lowering downstream costs.
  • Oligos up to 300nt make space for additional experimental elements. Add multiple guides per oligo, genome homology, or minimal promoters with ease.

Next generation CRISPR screens based on single cell transcriptomics

Watch our webinar: Next generation CRISPR screens based on single-cell transcriptomics

Genome tiling libraries

Tiling libraries allow a comprehensive study of genomic functionality. Pools of oligos may encode peptide arrays, mRNA encoding sequences, or genome fragments.

Product benefits include:

  • Tiling precise genomic regions of interest. Reduce experimental noise by ignoring unwanted sequences at the design step.
  • Complex, repetitive or palindromic sequences that cannot typically be synthesized as genes can be encoded in oligos.
  • Larger peptide structures or genome architectures can be encoded in oligos 300nt in length.
  • Uniform synthesis minimizes oversampling required for comprehensive screening.

Identifying Coronavirus Virulence Factors for RNA Vaccine Development

Read our article: Identifying Coronavirus Virulence Factors for RNA Vaccine Development with Twist Oligo Pools

High throughput genetic control screens

Understanding the genetic elements controlling gene expression and regulation has broad application in synthetic biology and development of potential disease treatment. Oligo Pools power high-throughput screens of genetic control, including gene expression profiling and reporter-based applications. 

Product benefits include:

  • High-quality pools ensure you can interrogate every base for functionality
  • High uniformity means you screen less to find your answers
  • Up to 300nt pools gives you the flexibility to add multiple elements or homology regions

Epitranscriptome Screening on TechNetworks

Watch our webinar: Epitranscriptome Screening on TechNetworks

Fluorescence in-situ hybridization

Fluorescence in-situ hybridization (FISH) allows the abundance and spatial organization of nucleic acids in biological samples to be directly visualized. Oligo Pools can encode probes that when bound to fluorescent markers provide tunable, programmable visualization of large numbers of targets.

Product benefits include:

  • Rapid FISH probe development
  • Sequence control enables effective targeting
  • Longer oligos enable more specific targeting

Rapid and efficient production of FISH probes using oligo pools with Nicola Crosetto

Watch our webinar: Rapid and efficient production of FISH probes using oligo pools with Nicola Crosetto

Specs
Oligo Length Up to 300nt
Oligo Pool Size No limits on pool size
Yield >0.2 fmol of each oligo
Uniformity >90% oligos represented within <2.5x of the mean
Error rate Up to 1:2000
Turn-Around Time Starting from 5 business days
Price Industry-leading pricing
Oligo Length Up to 300nt
Oligo Pool Size No limits on pool size
Yield >0.2 fmol of each oligo
Uniformity >90% oligos represented within <2.5x of the mean
Error rate Up to 1:2000
Turn-Around Time Starting from 5 business days
Price Industry-leading pricing
Resources
What Scientists Have to Say
Thanks for your help with our Oligo Pools order! The service and turn-around at Twist is really fantastic and we are already cloning full-speed.
Johannes Zuber
Professor • Research Institute of Molecular Pathology, Medical University Vienna
We have found Twist Oligo Pools to be a fast, convenient and flexible route to synthesizing high quality customized CRISPR libraries for our research.
Stephen Pettit
Staff Scientist • Institute of Cancer Research, UK
We work on massively parallel reporter assays (MPRAs) focused on identifying gene regulatory functions of single base substitutions, so high oligo fidelity is critical. When we sequenced the pool that we got from Twist the quality was astounding, with twofold fewer mutations than we were expecting.
Bernie Mulvey
MD-PhD Student • Washington University School of Medicine • St. Louis, MO, USA

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