Precision Synthesis of Variant Libraries Enables Comprehensive Interrogation of Single-Site Variant Space

Single Site Variant Libraries (SSVL) enable the interrogation of sequence space to identify key residues in protein structure and function. Traditional methods of generating variant diversity, such as error- prone PCR and site-directed mutagenesis using degenerate or semi-degenerate oligonucleotides, generate only a random sampling of mutations in the gene sequence.

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