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Twist 通用接头系统提高了面向新一代测序 (NGS) 应用的任何“T-A”末端连接文库制备工作流程的性能。该系统旨在优化 Twist 文库制备和靶向富集产品的性能,由 Twist 通用接头和 Twist 唯一双标签 (UDI) 引物或 Twist HT UDI 引物组成。
Twist 通用接头
The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step.
Twist Universal Adapter System (10 bp)
Twist 的 UDI 引物包含混合引物对,与组合方式相比,能够实现更可靠的样本混合。标签设计支持在测序后处理期间,更正标签序列中的替换、插入和缺失。
Twist HT Universal Adapter System (12 bp)
Twist 的 HT UDI 引物具有便于自动化的 96 孔板或 384 孔板规格,为高通量测序提供更具扩展性的解决方案。All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Twist Full Length UDI Adapters (10 bp) - NEW
Twist's latest NGS adapter sets are full length UDI adapters (10 bp in length) that enable PCR-free whole genome sequencing (WGS). With 1,536 adapters available, users have the ability to multiplex and to maximize the efficiency of utilization of sequencing reads in a sequencing run. 96 孔板配置旨在实现自动化,可以减少手动操作时间和人为的错误。
