Twist 表达抗体缓解了全长抗体表达的传统研究瓶颈。Twist 利用表达基因寡核苷酸合成平台提供无缝体验，在同一平台生产出经过 NGS 序列验证的克隆基因，可在哺乳动物细胞中转染和表达。我们的自动化生产线能够在短短 2 周内生产 10 到 1000 种 IgG 和 VHH 抗体。

Structure-based machine learning was leveraged to design predicted antibody binders against the GPCR target C5aR1. The CDR sequences of these predicted binders were then used to construct a de-novo phage-display library. Twist’s oligo-synthesis platform enabled fabrication of a highly diverse CDR-shuffle library with excellent variant representation and with no unwanted bias or motifs. Following panning, several high-affinity leads were identified that functionally blocked C5aR1 signaling in cellular assays.  This symposium was part of the event Antibody Engineering & Therapeutics Europe 2024.

（以日语发言）