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The Twist Methylation Target Enrichment with Fast Hybridization Protocol uses the Fast Hybridization Target Enrichment workflow to generate target-enriched DNA libraries for sequencing on Illumina next-generation sequencing (NGS) systems. 该方案详细介绍了高通量靶向富集系统的操作步骤,旨在通过最多 2 小时的杂交,特异性地靶向目标序列。A component of the Twist Targeted Methylation Sequencing solution, this protocol is:
- Designed for single or multiplex hybridization reactions using either Twist fixed or custom methylation panels; optional secondary methylation panels (spike-ins) can be added for additional content
- Optimized for use with the Twist-supplied NEBNext Enzymatic Methyl-seq v2 Library Preparation Kit
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