使用 UMI 接头系统的 Twist RNA 文库制备

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This Twist RNA Library Preparation Protocol details all the necessary steps to build sequencing libraries for target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. The steps include converting RNA to double-stranded cDNA and preparing cDNA libraries using the Twist UMI Adapter System for sequencing. Twist UMI 接头系统由 Twist UMI 接头和 Twist 唯一双标签 (UDI) 引物组成。This protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.

本方案涵盖的内容

RNA 靶向富集工作流程

包括用户提供的材料、常规说明和注意事项

基因

寡核苷酸池

NGS 靶向富集解决方案

基因突变文库

抗体发现

使用 UMI 接头系统的 Twist RNA 文库制备

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杂交捕获测序能比扩增子测序更可靠地进行病毒监测

SARS-CoV-2 NGS 检测