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This Twist RNA Library Preparation Protocol details all the necessary steps to build sequencing libraries for target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. The steps include converting RNA to double-stranded cDNA and preparing cDNA libraries using the Twist Universal Adapter System. Twist 通用接头系统由 Twist 通用接头和 Twist 独立双端标记 (UDI) 引物组成。该试剂盒还与 Twist HT 通用接头系统兼容,该系统由 Twist 通用接头和 Twist HT UDI 引物组成。This protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.
