Next-generation sequencing (NGS) offers the scalability, speed, and resolution to evaluate targeted genes of interest. 靶向组合具有核心内容，有助于靶向仅需要的基因集，从而降低检测成本。MedGenome labs has been performing exome testing and targeted panel testing by NGS over the last 8 years and have so far tested more than 100,000 samples. The challenge we face in the cost sensitive Indian market is in giving low cost tests that can be used by even the lower income group. We take pride in being the first to introduce the clinical exome panel (targeting only the clinically relevant genes) to the Indian market thereby ensuring the patients get a cost-effective, at the same time, clinically productive NGS test. We have been using the TWIST comprehensive exome the last two years the content of which has been well evaluated by us. It’s content is comparable and at times better than most of the currently available exomes in the market. Our diagnostic yield with the exomes has been comparable to the other exomes we were using till then and also comparable to the published data. We are also using 10 different TWIST targeted panels which gives us a cost-effective alternative to exomes. TWIST targeted somatic panels work well with the very bad quality FFPE too, with lesser failures due to bad quality data. Our experience with the team for panel designing and troubleshooting also have been excellent. To conclude, our experience with TWIST exomes and targeted panels have been very good and it offers us a good cost-effective option needed for the cost-sensitive Indian market.

在该海报中，我们展示了 Twist 的靶向富集系统。作为一种灵敏且信息量丰富的方法，它结合了文库制备和 30 分钟快速杂交技术，可检测 RNA 或 DNA 以及单链或双链的病毒。数据表明，我们的 SARS-CoV-2 研究组合可以用仅有 10 份拷贝的 SARS-CoV-2 基因组检测出病毒。将研究结果扩展到单一感染之外后，数据显示 Twist 呼吸道病毒研究组合能同时检测多种病毒，然后可在共同感染模型中以出色的覆盖度对其测序。

仅供研究使用。不适用于诊断程序。