This Twist RNA Library Preparation Protocol details all the necessary steps to build sequencing libraries for target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. The steps include converting RNA to double-stranded cDNA and preparing cDNA libraries using the Twist Universal Adapter System. Das Twist Universal Adapter System besteht aus Twist Universal Adaptern und Twist Unique Dual Indexed (UDI) Primern. Dieses Kit ist auch mit dem Twist HT Universal Adapter System kompatibel, das aus Twist Universal Adaptern und Twist HT UDI Primern besteht. This protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.