The simple, fast, most cost-effective way to get linear dsDNA. Our industry-leading synthesis quality means less colony screening to find the perfect clone.
La tecnología de síntesis de ADN de Twist supera a la competencia con tasas de error excepcionalmente bajas.
In this study, Twist Gene Fragments have an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over IDT eBlocks™ and gBlocks™ Gene Fragments.
Get a higher percentage of perfect clones with Twist Gene Fragments, reducing your time screening colonies.
In this study of 63 unique sequences, our low error rate meant Twist Gene Fragments consistently yielded the highest percentage of perfect clones when compared to IDT eBlocks™ and gBlocks™ Gene Fragments. Cloning efficiency is a crucial factor for determining sequencing costs, which can add up when screening colonies.
Colony picking calculator
Our industry-leading fragment synthesis error rate means you need to pick fewer colonies to find the perfect clone. Estimate how many colonies you need to pick here*.
*Colony numbers vary depending on sequence complexity, purity, toxicity, and cloning method. In some cases, picking 2–3 times more colonies than estimated here may be necessary.
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Especificaciones del producto
| Specification | Value | Detalles |
|---|---|---|
| Product format | Linear dsDNA | |
| Longitud | 0,3 kb-5,0 kb | |
| Complejidad | Bajo | Medio |
A sequence's complexity score is based on the combined presence, frequency, and length of various features within the design of a sequence. Impacts the ease and speed of production. High complexity sequences are available as Clonal Genes. |
| Optimización del codón | Large language model-based | Available for protein expression in hundreds of target organisms. |
| Amplification adapters Add-on |
|
Available to be appended to the 5' and 3' ends of all sequences, allowing for single primer set amplification. 5’– CAATCCGCCCTCACTACAACCG-<insert sequence>-CTACTCTGGCGTCGATGAGGGA – 3’ |
| Specification | Value | Detalles |
|---|---|---|
| Tiempo de entrega | 2 – 4 business days* | |
| Rendimiento | 100 ng a 1 µg | |
| Container | 2 mL tube, 96-well plate or 384-well plate |
|
| Concentration normalization Add-on | 250 ng/fragment | |
| Delivery format Add-on | Dried down or resuspended |
|
| Shipment conditions | Ambient or frozen on dry ice |
|
Every Gene Fragment we ship includes a Certificate of Analysis (COA) that contains the following information.
| Specification | Value | Detalles |
|---|---|---|
| Yield measurement | Fluorometric quantification | |
| Verificación de longitud | Capilliary electrophoresis |
Customers often pair Twist Genes with the following products
Asóciese con nuestro equipo de expertos para recorrer todo el embudo de descubrimiento, desde la diana hasta el candidato principal.
Grupos de oligonucleótidos de alta diversidad, sintetizados en silicio, diseñados para el cribado de alto rendimiento y la construcción de bibliotecas complejas.
Explore un amplio espacio de secuencias con bibliotecas de alta diversidad en las que cada variante se sintetiza base por base y se verifica mediante NGS para que coincida con su diseño.
El equipo de soporte técnico de Twist está disponible para ayudarle a enfrentarse a los mayores retos de la investigación. If you have any questions about your project, our scientists are here to find the answer.
Solo para investigación.
No diseñados para procedimientos diagnósticos.
* Este plazo hace referencia al tiempo de manipulación y de procesamiento típicos en nuestras instalaciones antes de que su pedido se entregue al transportista. Gene Fragments are shipped in 2-4 business days. Orders placed outside of the US will incur additional delivery time.
†This is for illustrative purposes and the stated benefits, support, benefit calculations and other results are estimates only, based on input from a limited number of existing Twist customers and various assumptions made by the potential customers. This estimate does not constitute a proposal or quote.
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
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