This Twist RNA Library Preparation Protocol details all the necessary steps to build sequencing libraries for target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. The steps include converting RNA to double-stranded cDNA and preparing cDNA libraries using the Twist Universal Adapter System. El sistema de adaptador universal Twist consta de adaptadores universales Twist y cebadores de índice doble únicos (UDI) Twist. Este kit también es compatible con el sistema de adaptador universal de alto rendimiento Twist, que consta de adaptadores universales Twist y cebadores de índice doble único (UDI) de alto rendimiento Twist. This protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.