First published in 2019, the technique of prime editing offers significant advantages over other genome editing methods, such as CRISPR/Cas9. One critical benefit is the introduction of single-strand, rather than double-strand breaks in the target DNA, which allows the more accurate mismatch repair mechanism to be deployed — reducing the number of unwanted and erroneous edits. In addition, prime editing can install a wider range of edits, including transitions (interchanges of purines or of pyrimidines) and transversions (purines to pyrimidines, and vice versa), as well as deletions and insertions.  Despite its promise, the factors that govern the successful integration of short sequences to a genomic target using prime editing remain unclear. En este seminario web, investigadores de Wellcome Sanger Institute, Reino Unido, describirán su innovador trabajo en el que analizan miles de secuencias de inserción en diferentes contextos genómicos para comprender y predecir las eficiencias de inserción de la edición primaria. Los resultados son específicos de la institución en la que se han obtenido y pueden no reflejar los resultados que puedan obtenerse en otras instituciones. Solo para investigación.

We find ourselves at an inflection point in functional genomics, one characterized by precision, expanding functionality, and high-throughput capabilities. With this comes a need for high performance tools and experts in the field to help researchers navigate the changing technological landscape and create successful experiments. This eBook offers a contemporary synthesis of CRISPR screening technologies for functional Geneticists, as well as advice from our CRISPR experts.

Hear from Professor Hyongbum Henry Kim about his exciting research work around CRISPR and Prime editing. He will also take the opportunity to share more about Combining Massive Oligo Pools and Artificial Intelligence (AI) to Predict Guide RNA Efficiency for Prime Editing Screens.