Toward a molecular system capable of self-regeneration

Norikazu Ichihashi
Presentado por
Norikazu Ichihashi
The University of Tokyo, Japan

Cubierto en este seminario web
Reproduction of 20 types of aminoacyl-tRNA(20種類のアミノアシルtRNAの再生産)
A novel method for synthesizing tRNA in vitro in bulk(tRNAをin vitroで一気にまとめて合成する新しい方法)
Production of ribosomes lacking rRNA chemical modifications(rRNAの化学修飾を欠損したリボソームの作出)

This seminar explores recent progress toward a self-reproducing molecular system based on in vitro DNA replication, transcription, and translation. Alongside the latest results, the talk will share practical tips for cell-free research, including efficient factor expression in the E. coli PURE system, strategies for generating homogeneous RNA, and a new method to synthesize 21 unmodified tRNAs simultaneously. A concise and accessible talk for researchers interested in cell-free systems and synthetic biology.

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