On the Custom Vector On-boarding form, what is meant by Vector Sequence, Insert Sequence, and Vector + Insert Sequence?

The Vector sequence is the complete sequence of the vector you ship to us. We use this information for sequence QC and to confirm the cloning strategy.

The Insert Sequence is an example of all future inserts you want Twist to make and tells us exactly where you want your gene inserts to begin and end in the Vector + Insert sequence.  100% of the Insert Sequence must be found in the Vector + Insert sequence.  Note that the insert sequence will not be made as part of the vector onboarding process.  

The Vector + Insert Sequence is an example of what you want your final product to look like. We should be able to find 100% of the base pairs of the insert sequence in the Vector + Insert Sequence.

 

Was this article helpful?

No

Still have questions? Contact Us