If I use restriction enzymes for cloning, how do I submit my custom vector onboarding form?

There are two options for using restriction enzyme cloning.

Option 1 - Include Restriction Sites on the insert

When filling out the Insert box in the custom vector submission form, include the whole restriction enzyme site on either end of the insert sequence. The restriction enzyme sites will be part of the inserts in silico.

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When you order genes, you must include restriction enzyme sites on the inserts.  In this custom vector onboarding scenario, if you exclude restriction enzyme sites on the genes in your order, the restriction enzyme sites will be completely absent from your final product.

 

Option 2 - Include Restriction Sites on the vector

When filling out the Insert box in the custom vector submission form, do not include restriction enzyme sites on the insert sequence. Include each whole restriction enzyme site on the Vector + Insert box in Custom Vector onboarding form. The restriction enzyme sites will be part of the vector in silico.

 

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When you order genes, do not include restriction enzyme sites on the inserts.  In this custom vector onboarding scenario, if you include restriction enzyme sites on the genes in your order, the restriction enzyme sites will be duplicated in your final product.

 

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