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Twist EM-seq v2 Methylation Detection System
- 概要
- 資料
- アプリケーション
Twist EM-seq v2 Methylation Detection System enables accurate methylation analysis through a fully enzymatic workflow designed to preserve DNA integrity while delivering consistent library yields and high conversion efficiency. Optimized for low input and challenging samples, EM-Seq v2 supports robust methylation profiling without bisulfite induced DNA damage.
製品シート
プロトコル
製品データ
Engineered reagents preserve performance post-conversion
Libraries prepared with EM-Seq v2 maintain capture efficiency and uniformity. Performance is preserved through downstream workflows specifically for methylation applications, including compatibility with both standard and fast hybridization protocols.
Consistent Methylation Hybridization Capture Performance
EM-Seq v2 libraries processed with Twist hybridization maintain consistent capture efficiency and methylation performance, supporting reliable downstream analysis.
Reproducible Methylation Profiling Across Targets
Consistent detection of differentially methylated regions demonstrates reliable performance across methylation targets, reinforcing reproducibility for downstream methylation analysis.
Consistent library yield across DNA inputs
EM-Seq v2 generates consistent, usable library yields across a wide DNA input range. Reduced dropout at lower inputs supports methylation sequencing when sample availability is limited, while maintaining reliable performance across workflows.
Confident Enzymatic Conversion for Accurate CpG Methylation Calling
EM-Seq v2 delivers robust enzymatic conversion of unmethylated cytosines with consistent CpG call ratios, supporting accurate and reliable methylation analysis while preserving DNA integrity.
図 1. The Twist Methylation Enhancer improves target enrichment quality and reduces methylation-specific off-target hybridization for Twist EM-Seq v2 libraries captured with the Twist Alliance Pan-Cancer panel, using either the Fast or Standard Hybridization v2 systems.
図 2. Twist EM-Seq v2 Methylation Detection System libraries captured with the Twist Alliance Pan-Caner panel using the Twist Standard Hybridization v2 system provide highly reproducible Picard target enrichment performance metrics across samples with highly variable methylation levels.
図 3. Highly sensitive methylation detection. Detection of methylation is possible across a wide range of methylation levels and targets.
図 4. Yield of libraries generated with Twist EM-seq v2 Methylation Detection System. Library yields are high even with low mass input.
図 5. Left Panel: Non-CPG Conversion Rate. Conversion rates with v2 are high even with low mass input. Right Panel: Called CPG Ratio. Conversion specificity with v2 is high even with low mass input.
Engineered reagents preserve performance post-conversion
Libraries prepared with EM-Seq v2 maintain capture efficiency and uniformity. Performance is preserved through downstream workflows specifically for methylation applications, including compatibility with both standard and fast hybridization protocols.
図 1. The Twist Methylation Enhancer improves target enrichment quality and reduces methylation-specific off-target hybridization for Twist EM-Seq v2 libraries captured with the Twist Alliance Pan-Cancer panel, using either the Fast or Standard Hybridization v2 systems.
Consistent Methylation Hybridization Capture Performance
EM-Seq v2 libraries processed with Twist hybridization maintain consistent capture efficiency and methylation performance, supporting reliable downstream analysis.
図 2. Twist EM-Seq v2 Methylation Detection System libraries captured with the Twist Alliance Pan-Caner panel using the Twist Standard Hybridization v2 system provide highly reproducible Picard target enrichment performance metrics across samples with highly variable methylation levels.
Reproducible Methylation Profiling Across Targets
Consistent detection of differentially methylated regions demonstrates reliable performance across methylation targets, reinforcing reproducibility for downstream methylation analysis.
図 3. Highly sensitive methylation detection. Detection of methylation is possible across a wide range of methylation levels and targets.
Consistent library yield across DNA inputs
EM-Seq v2 generates consistent, usable library yields across a wide DNA input range. Reduced dropout at lower inputs supports methylation sequencing when sample availability is limited, while maintaining reliable performance across workflows.
図 4. Yield of libraries generated with Twist EM-seq v2 Methylation Detection System. Library yields are high even with low mass input.
Confident Enzymatic Conversion for Accurate CpG Methylation Calling
EM-Seq v2 delivers robust enzymatic conversion of unmethylated cytosines with consistent CpG call ratios, supporting accurate and reliable methylation analysis while preserving DNA integrity.
図 5. Left Panel: Non-CPG Conversion Rate. Conversion rates with v2 are high even with low mass input. Right Panel: Called CPG Ratio. Conversion specificity with v2 is high even with low mass input.
製品データ
Enzymatic Methylation Conversion with EM-Seq v2
EM-Seq V2 uses enzymatic reactions to selectively convert unmethylated cytosines while protecting methylated bases. After amplification, unmethylated sites are read as thymine and methylated sites remain cytosine, enabling accurate methylation analysis without DNA damage.
注目すべきリソース
Product Sheet関連ワークフローとアプリケーション
ターゲットエンリッチメントワークフロー
Target enrichment workflows with Twist deliver scalable, low bias, and highly uniform coverage across large and complex genomic regions; enabling broader discovery, higher sensitivity, and greater flexibility than amplicon or array-based methods.
リキッドバイオプシーでの応用
Twist はリキッドバイオプシーやがんの早期特性解析に関する研究を支えるさまざまなツールを提供しています。
ライブラリ調製ソリューション
Library PrepTwist Library Preparation Kit は、主な調製プロセスを単一の効率的な反応に統合することで、次世代シーケンシング（NGS）用高品質 DNA ライブラリの構築を効率化します。
詳細はこちら 
Oncology - DNA CGP Panel
ターゲットエンリッチメントパネルTwist DNA CGP Panel は、SNV、インデル、コピー数多型、および遺伝子再構成を包括的に検出することができ、1 回のアッセイで広範な変異をカバーします。
詳細はこちら
MRD Rapid 500 Panel
ターゲットエンリッチメントパネルTwist Bioscience の MRD Rapid 500 Panel は、微小残存病変（MRD）を対象としたスケーラブルなターゲットエンリッチメントソリューションであり、50 ～ 500 プローブからなるパネルを用いて広範なゲノムカバレッジを実現します。
詳細はこちら
Twist Bioscience本社
681 Gateway Blvd
South San Francisco, CA 94080
