人工遺伝子
オリゴプール
NGS
変異体ライブラリ
抗体
IVDR ソリューション
Twist Bioscience本社
681 Gateway Blvd
South San Francisco, CA 94080
Reliable, scalable cfDNA workflows
Generate high-quality libraries from challenging cfDNA inputs with a streamlined workflow that delivers high conversion and sensitivity for rare variant detection. Designed for low input and fragmented samples, the Twist cfDNA Library Prep Kit helps you uncover critical insights with confidence and efficiency.
レアバリアントの検出感度を最大化
高変換効率のため、レアバリアント（≦ 0.1% VAF）を確実に検出。Support duplex sequencing workflows with the Twist UMI Adapter System to maximize detection sensitivity.
Streamlined workflow for precious samples
Achieve robust performance, even with low (<1 ng) sample input. Get sequence-ready libraries in 2 hours.
バイアスのない増幅
Get consistent coverage across 5%–95% GC, preserving challenging genomic regions and difficult-to-capture targets. Get consistent high yields from complex libraries, even at ≤100 pg of DNA input. Call variants with confidence.
製品データ
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Prep Kit was used to generate libraries with the Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full-Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. PCR 条件としては、Twist TrueAmp Polymerase Mix および UDI プライマーを用いて、6 サイクルで増幅を行った。ライブラリをプールし、シーケンシングを行って分析した。PCR なしで構築したライブラリと TrueAmp を用いたライブラリの間で、同程度のエラー率が見られる。
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
図 2. The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water, and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). 未検出バリアントとは、検出可能な全部位数に対してバリアントファミリーが 1 つ未満の部位を指します。
図 3. The Twist cfDNA Library Prep Kit enables sensitive and accurate detection of variants at 0.25% VAF. ライブラリは、Pan-Cancer Standard サンプルにおける 217 個の SNP 変異部位を標的とするカスタム 50 kb オンコロジーパネルを用いてシングルプレックスでキャプチャ。「Variant Missed」（検出されなかったバリアント率）は、バリアントファミリーが 1 つ未満の部位の合計を、検出可能なバリアント部位の総数で割ることで算出されます。
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
図 4. The Twist cfDNA Library Prep Kit delivers higher complexity with and without UMI deduplication.
ビデオ
High-fidelity amplification with PCR
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
Figure 1 Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Prep Kit was used to generate libraries with the Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full-Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. PCR 条件としては、Twist TrueAmp Polymerase Mix および UDI プライマーを用いて、6 サイクルで増幅を行った。ライブラリをプールし、シーケンシングを行って分析した。PCR なしで構築したライブラリと TrueAmp を用いたライブラリの間で、同程度のエラー率が見られる。
高変換率 = 高収量
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
図 2. The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water, and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
より高感度かつ高精度
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). 未検出バリアントとは、検出可能な全部位数に対してバリアントファミリーが 1 つ未満の部位を指します。
図 3. The Twist cfDNA Library Prep Kit enables sensitive and accurate detection of variants at 0.25% VAF. ライブラリは、Pan-Cancer Standard サンプルにおける 217 個の SNP 変異部位を標的とするカスタム 50 kb オンコロジーパネルを用いてシングルプレックスでキャプチャ。「Variant Missed」（検出されなかったバリアント率）は、バリアントファミリーが 1 つ未満の部位の合計を、検出可能なバリアント部位の総数で割ることで算出されます。
重複率の改善 = 複雑性の高さ
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
図 4. The Twist cfDNA Library Prep Kit delivers higher complexity with and without UMI deduplication.
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High-fidelity amplification with PCR
High-fidelity amplification with Twist TrueAmp Polymerase generates cfDNA libraries that have substitution error rates comparable to those of PCR-free cfDNA libraries across all analyzed base changes.
高変換率 = 高収量
The Twist cfDNA Library Prep Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 μL of water, and 1 µL was quantified with Qubit™ dsDNA Broad Range Kit.
より高感度かつ高精度
The Twist cfDNA Library Prep Kit enables sensitive, accurate detection down to 0.25% VAF. Single-plex capture with a 50 kb oncology panel targets 217 single nucleotide polymorphisms (SNPs). 未検出バリアントとは、検出可能な全部位数に対してバリアントファミリーが 1 つ未満の部位を指します。
重複率の改善 = 複雑性の高さ
The Twist cfDNA Library Prep Kit delivers higher library complexity with or without unique molecular identifier (UMI) deduplication. Single-plex capture with a 50 kb oncology panel targets variant sites in cfDNA standard material using optimized target enrichment workflows.
