TrueAmp Library Preparation Kit

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Uniform coverage and confident variant detection

Produce consistent, high-quality libraries for next generation sequencing (NGS) target enrichment workflows with the TrueAmp Library Prep Kit. Powered by the Twist TrueAmp polymerase and optimized for enzymatic fragmentation, this workflow delivers high conversion efficiency and low GC bias. These features give balanced coverage across challenging genomic regions, providing the accuracy needed for reliable variant characterization.

Reliable uniformity across challenging regions

Improve coverage consistency to reduce AT- and GC-related dropout. Ensure better representation of difficult genomic regions for more comprehensive sequencing results.

Efficient sample conversion

Maximize library yields from low-input, formalin-fixed, paraffin-embedded (FFPE), and variable-quality samples. Preserve precious starting material and minimize the need for reruns.

High-fidelity amplification

Minimize amplification-induced errors and bias with the Twist TrueAmp polymerase. Achieve accurate detection of low-frequency variants to increase confidence in every call.

製品データ

The TrueAmp Library Prep Kit delivers higher pre-capture yield, consistent library sizes, and stronger mean target coverage from low input, degraded FFPE DNA, preserving library complexity and increasing reliable sequencing results from challenging samples.

The Twist TrueAmp Library Prep Kit delivers improved coverage uniformity and fewer zero coverage regions. Reduced target dropout increases confidence that critical regions are consistently sequenced.

Twist TrueAmp Polymerase Mix は、GC 含量の高い領域と低い領域の双方でより均一なカバレッジを実現し、PCR サイクル数を増やしてもパフォーマンスを維持します。

The Twist TrueAmp Library Prep Kit delivers consistent DNA library fragment sizes and sufficient yield across a wide range of sample inputs, enabling mixed input workflows while maintaining uniform coverage and reproducible results at scale.

Twist TrueAmp Library Prep Kit delivers repeatable DNA library fragment sizes across a broad range of input amounts supporting confidence in sequencing results and downstream analysis.

ビデオ

Improved NGS library yield, complexity, and coverage from low-input FFPE samples using high-efficiency library preparation for degraded DNA sequencing

Enhanced coverage uniformity and reduced zero-coverage regions in FFPE sequencing libraries with optimized NGS library preparation workflow

Reduced GC bias in sequencing libraries with high-fidelity polymerase showing uniform coverage across GC content at multiple PCR cycle conditions

Consistent library size and high yield from ultra-low DNA input samples demonstrating robust NGS library prep performance across input ranges

Tunable DNA fragment size and insert length control in NGS library preparation using adjustable enzymatic fragmentation timing

最も解析困難なサンプルに対する高収量と高カバレッジ

図 1. Performance comparison of enriched libraries with low-input FFPE degraded samples (DIN <2.2) between TrueAmp Library Prep Kit and competitor K kit, demonstrating the optimal solution for challenging sample applications. (A) The Twist TrueAmp Library Prep Kit generates superior pre-capture library yield, indicative of high library construction and amplification efficiency. (B) The Twist TrueAmp Library Prep Kit shows higher library complexity when compared with the competitor’s kits. これにより、ライブラリ内でシーケンシング可能なユニーク DNA 分子の数を増やすことができ、シーケンシングコストを削減できる。(C) より高いカバレッジを実現します。

より少ないターゲットで均一なカバレッジ

The Twist TrueAmp Library Prep Kit delivers improved coverage uniformity and fewer zero coverage regions. Reduced target dropout increases confidence that critical regions are consistently sequenced.

図 2. Performance comparison of enriched libraries with low-input FFPE degraded samples (DIN <2.2) between TrueAmp Library Prep Kit and competitor K kit, demonstrating the optimal solution for challenging sample applications. (A) 優れたカバレッジ均一性を提供し、それは低いfold-80ベースペナルティの測定で表されます。(B) カバレッジのない領域が縮小しており、それはゼロカバレッジターゲット率の測定で表されます。

広範囲の GC 領域に対するカバレッジを改善

図 3. Twist TrueAmp ポリメラーゼのカバレッジが向上したことを示す、GC バイアスの正規化トレース。Libraries were prepared with Twist TrueAmp Library Prep Kit and amplified with different polymerases and cycles. 上部パネル：PCR を 3 サイクル行った場合のポリメラーゼの比較を示す、GC ウィンドウに対する正規化カバレッジ。下部パネル：PCR を 16 サイクル行った場合のポリメラーゼの比較を示す、GC ウィンドウに対する正規化カバレッジ。

Built for consistent fragment sizes across inputs and workflows

図 4. Reliable library size with Twist TrueAmp Library Prep Kit, even from ultra-low inputs. 500 ng, 100 ng, 50 ng, 10 ng, and 1 ng (gDNA) were fragmented at 32°C. 3, 5, 6, 8, 10, and 14 cycles of PCR were used for amplification, respectively. 各サンプルは 2 回ずつ測定を行った。(A) Electropherograms of NGS libraries generated with the Twist TrueAmp Library Prep Kit. (B) Concentration of libraries after amplification for various DNA inputs.

高性能な調整のために制御された挿入サイズ

Twist TrueAmp Library Prep Kit delivers repeatable DNA library fragment sizes across a broad range of input amounts supporting confidence in sequencing results and downstream analysis.

図 5. Twist TrueAmp Library Preparation Kit のチューナビリティ。(A) Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C. 6 cycles of PCR were utilized for amplification. (B) Median insert size vs time. 50 ng of high-quality gDNA was fragmented for various times at 32°C. 増幅は、6 サイクルの PCR を用いて実施。サンプルは Twist Exome 2.0 パネルを用いてキャプチャした。

最も解析困難なサンプルに対する高収量と高カバレッジ

より少ないターゲットで均一なカバレッジ

The Twist TrueAmp Library Prep Kit delivers improved coverage uniformity and fewer zero coverage regions. Reduced target dropout increases confidence that critical regions are consistently sequenced.

広範囲の GC 領域に対するカバレッジを改善

Built for consistent fragment sizes across inputs and workflows

高性能な調整のために制御された挿入サイズ

Twist TrueAmp Library Prep Kit delivers repeatable DNA library fragment sizes across a broad range of input amounts supporting confidence in sequencing results and downstream analysis.