概要 TCR LIBRARIES CAR LIBRARIES ご注文 資料
概要

TCR and CAR Libraries 

Partner with Twist Bioscience to identify and develop novel advanced cell therapies through large-scale, highly diverse T-cell Receptor (TCR), chimeric antigen receptor (CAR), and multispecific VHH libraries all fueled by our combinatorial assembly methodology. This methodology utilizes seamless assembly of multiple variant domains to rapidly create libraries that enable high-throughput screening and characterization of novel and known variants for therapeutic discovery.

Provide us your customized, user defined gene parts, by filling out our library’s submission form and send it to [email protected]. Following, we will review your sequences, help you optimize them if necessary, and then get to work building your libraries! Expect to receive your highly uniform TCR and CAR libraries in as little as 8 -12 weeks. 

Before you know it, you too will be able to screen for and identify desired combinations of custom gene parts within TCR, CAR or antibody VHH/scFv domains. 

Learn more about each of our libraries in the corresponding TCR and CAR tabs on this webpage! 

多様性に富むライブラリ
多様性に富むライブラリ
各コンビナトリアル・アセンブリは、最大 10,000 個の二本鎖DNA断片を組み合わせて構成可能
お客様のライブラリをカスタマイズ
お客様のライブラリをカスタマイズ
TCR:α 鎖や β 鎖にユーザー定義のコンビナトリアル変異を挿入
CAR:ヒンジ、膜貫通、シグナル伝達ドメインにユーザー定義のコンビナトリアル変異を挿入
高品質
高品質
平均値の 10 倍以内に 90% 超の変異体が存在する、すべて NGS で検証済みのライブラリ
ハイスループット
ハイスループット
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
フレキシブル
フレキシブル
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
ご不明な点はございますか?

より詳細な情報が必要な場合はお気軽にご連絡ください。当社におまかせください。

お問い合わせ

TCR and CAR Libraries 

Partner with Twist Bioscience to identify and develop novel advanced cell therapies through large-scale, highly diverse T-cell Receptor (TCR), chimeric antigen receptor (CAR), and multispecific VHH libraries all fueled by our combinatorial assembly methodology. This methodology utilizes seamless assembly of multiple variant domains to rapidly create libraries that enable high-throughput screening and characterization of novel and known variants for therapeutic discovery.

Provide us your customized, user defined gene parts, by filling out our library’s submission form and send it to [email protected]. Following, we will review your sequences, help you optimize them if necessary, and then get to work building your libraries! Expect to receive your highly uniform TCR and CAR libraries in as little as 8 -12 weeks. 

Before you know it, you too will be able to screen for and identify desired combinations of custom gene parts within TCR, CAR or antibody VHH/scFv domains. 

Learn more about each of our libraries in the corresponding TCR and CAR tabs on this webpage! 

多様性に富むライブラリ
多様性に富むライブラリ
各コンビナトリアル・アセンブリは、最大 10,000 個の二本鎖DNA断片を組み合わせて構成可能
お客様のライブラリをカスタマイズ
お客様のライブラリをカスタマイズ
TCR:α 鎖や β 鎖にユーザー定義のコンビナトリアル変異を挿入
CAR:ヒンジ、膜貫通、シグナル伝達ドメインにユーザー定義のコンビナトリアル変異を挿入
高品質
高品質
平均値の 10 倍以内に 90% 超の変異体が存在する、すべて NGS で検証済みのライブラリ
ハイスループット
ハイスループット
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
フレキシブル
フレキシブル
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
ご不明な点はございますか?

より詳細な情報が必要な場合はお気軽にご連絡ください。当社におまかせください。

お問い合わせ

TCR Libraries

ハイスループット抗体生産とは

Adoptive cell therapy (ACT) has led to highly effective cancer treatments for patients who had very few treatment options. It utilizes the patient’s own immune system to attack cancer cells. 

Engineered TCR therapy is a type of ACT that leverages engineered T cell receptors to target tumor specific antigens. This starts with sequencing of the tumor biopsy to identify tumor mutations, and typically, the peripheral blood to uncover the TCR repertoire. 

TCR repertoire sequencing can be done via single cell sequencing or bulk sequencing. Each has it’s own advantages. Bulk sequencing enables you to sample more of the sequence space but information about the alpha-beta TCR pairing is lost. Single-cell sequencing enables you to capture information on the alpha-beta chain pairing and receptor composition. However, single-cell sequencing has a much lower throughput than bulk sequencing.  

How can Twist help me build TCR libraries?

Twist offers TCR libraries that complements both strategies:

Paired Pooled TCR Library (figure 1A)

  • Enables you to retain the original alpha-beta chain pairing to maximize hit validation and replicate the TCR repertoire
  • This is a good complement to single-cell sequencing where the alpha-beta TCR pairing is known

Combinatorial TCR Library (figure 1B)

  • Enables you to shuffle alpha and beta chain pairs to create additional diversity and explore novel combinations beyond the identified repertoire
  • This is a good complement to bulk sequencing where the alpha-beta TCR pairing is unknown 
     

TCR figure

Performance Data

The discovery of TCRs is driven by the screening of immune cell repertoires and the re-synthesis of large numbers of potential binders. TCR Libraries can be leveraged to rapidly generate combinations of TCR beta and alpha chains for high throughput screening. With combinatorial TCR libraries, the alpha and beta chains are shuffled together to create a library of greater diversity to integrate the variant space beyond the identified repertoire. With paired pool TCR libraries, explicit alpha and beta chain pairing identified through sequencing are conserved. 

Combinatorial TCR Libraries

Figure 1

Paired Pool TCR Libraries

Figure 2

T Cell Receptor (TCR) Discovery workflow 

Partner with Twist Bioscience to identify and develop novel advanced cell therapies through large-scale, highly diverse T-cell Receptor (TCR) libraries.

antigen receptor

二本鎖 DNA 断片を精密かつユーザーが定義した通りに組み合わせた、高均一性のスクリーニングライブラリをご利用になることで、目的の組み合わせに対する網羅的なスクリーニングを効率的に行えます。

 

多様性に富むライブラリ
多様性に富むライブラリ
各コンビナトリアル・アセンブリは、最大 10,000 個の二本鎖DNA断片を組み合わせて構成可能
Customize Your TCR Library
Customize Your TCR Library
User defined combinatorial variants across alpha and beta chains
高品質
高品質
平均値の 10 倍以内に 90% 超の変異体が存在する、すべて NGS で検証済みのライブラリ
ハイスループット
ハイスループット
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
フレキシブル
フレキシブル
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
ご不明な点はございますか?

より詳細な情報が必要な場合はお気軽にご連絡ください。当社におまかせください。

お問い合わせ

ハイスループット抗体生産とは

Adoptive cell therapy (ACT) has led to highly effective cancer treatments for patients who had very few treatment options. It utilizes the patient’s own immune system to attack cancer cells. 

Engineered TCR therapy is a type of ACT that leverages engineered T cell receptors to target tumor specific antigens. This starts with sequencing of the tumor biopsy to identify tumor mutations, and typically, the peripheral blood to uncover the TCR repertoire. 

TCR repertoire sequencing can be done via single cell sequencing or bulk sequencing. Each has it’s own advantages. Bulk sequencing enables you to sample more of the sequence space but information about the alpha-beta TCR pairing is lost. Single-cell sequencing enables you to capture information on the alpha-beta chain pairing and receptor composition. However, single-cell sequencing has a much lower throughput than bulk sequencing.  

How can Twist help me build TCR libraries?

Twist offers TCR libraries that complements both strategies:

Paired Pooled TCR Library (figure 1A)

  • Enables you to retain the original alpha-beta chain pairing to maximize hit validation and replicate the TCR repertoire
  • This is a good complement to single-cell sequencing where the alpha-beta TCR pairing is known

Combinatorial TCR Library (figure 1B)

  • Enables you to shuffle alpha and beta chain pairs to create additional diversity and explore novel combinations beyond the identified repertoire
  • This is a good complement to bulk sequencing where the alpha-beta TCR pairing is unknown 
     

TCR figure

Performance Data

The discovery of TCRs is driven by the screening of immune cell repertoires and the re-synthesis of large numbers of potential binders. TCR Libraries can be leveraged to rapidly generate combinations of TCR beta and alpha chains for high throughput screening. With combinatorial TCR libraries, the alpha and beta chains are shuffled together to create a library of greater diversity to integrate the variant space beyond the identified repertoire. With paired pool TCR libraries, explicit alpha and beta chain pairing identified through sequencing are conserved. 

Combinatorial TCR Libraries

Figure 1

Paired Pool TCR Libraries

Figure 2

T Cell Receptor (TCR) Discovery workflow 

Partner with Twist Bioscience to identify and develop novel advanced cell therapies through large-scale, highly diverse T-cell Receptor (TCR) libraries.

antigen receptor

二本鎖 DNA 断片を精密かつユーザーが定義した通りに組み合わせた、高均一性のスクリーニングライブラリをご利用になることで、目的の組み合わせに対する網羅的なスクリーニングを効率的に行えます。

 

ご不明な点はございますか?

より詳細な情報が必要な場合はお気軽にご連絡ください。当社におまかせください。

お問い合わせ

多様性に富むライブラリ
多様性に富むライブラリ
各コンビナトリアル・アセンブリは、最大 10,000 個の二本鎖DNA断片を組み合わせて構成可能
Customize Your TCR Library
Customize Your TCR Library
User defined combinatorial variants across alpha and beta chains
高品質
高品質
平均値の 10 倍以内に 90% 超の変異体が存在する、すべて NGS で検証済みのライブラリ
ハイスループット
ハイスループット
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
フレキシブル
フレキシブル
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
CAR Libraries

ハイスループット抗体生産とは

Over the last few years, chimeric antigen receptor (CAR) T-cell therapy emerged as a new form of immunotherapy. Novel CAR designs coupled with advances in gene transfer technology and gene editing has opened the door for new cancer therapies. However, there are still many challenges that needs to be tackled to accelerate development.  

CARs consist of a extracellular binding domain, a hinge region, a transmembrane domian, and one or more intracellular domains. Fine-tuning each domain of the CAR is one of the initial steps to improving T cell specificity, antigen recognition, and T cell function. Studies have shown that small modifications to the CAR can have significant impact on the therapeutic outcome. Therefore, it key to have a comprehensive library to interrogate as each CAR construct needs intensive testing. 

Twist has developed a technology to build CAR libraries in a manner that seamlessly shuffles variants of each domain via scarless assembly process. 

当サービスの流れ

different sequence

Figure1: Each of these domains can consist of several different sequence variants. These domains are synthesized as genes which Twist combines together in a through scarless assembly process to generate a highly diverse library for scaffold optimization. This technology enables the discovery of unique domain combinations that reveal novel functionalities.

Chimeric Antigen Receptor (CAR) Discovery and Optimization Workflow

CAR の足場の各ドメインは、それぞれ独立して、また相乗的に CAR の機能に影響を与えます。CAR Libraries serve as a tool to fine-tune each of the modules to uncover codependencies and better understand the impact on T-cell specificity, antigen recognition and T-cell function.

Partner with Twist at any stage of your discovery workflow! 

  • Leverage both in vivo and in vitro workflows for binder discovery and optimization
  • Leverage our synthetic libraries for scaffold optimization and validation

T cell receptor

多様性に富むライブラリ
多様性に富むライブラリ
各コンビナトリアル・アセンブリは、最大 10,000 個の二本鎖DNA断片を組み合わせて構成可能
Customize Your CAR Library
Customize Your CAR Library
User defined combinatorial variants across hinge, transmembrane, and signaling domains
高品質
高品質
平均値の 10 倍以内に 90% 超の変異体が存在する、すべて NGS で検証済みのライブラリ
ハイスループット
ハイスループット
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
フレキシブル
フレキシブル
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
ご不明な点はございますか?

より詳細な情報が必要な場合はお気軽にご連絡ください。当社におまかせください。

お問い合わせ

ハイスループット抗体生産とは

Over the last few years, chimeric antigen receptor (CAR) T-cell therapy emerged as a new form of immunotherapy. Novel CAR designs coupled with advances in gene transfer technology and gene editing has opened the door for new cancer therapies. However, there are still many challenges that needs to be tackled to accelerate development.  

CARs consist of a extracellular binding domain, a hinge region, a transmembrane domian, and one or more intracellular domains. Fine-tuning each domain of the CAR is one of the initial steps to improving T cell specificity, antigen recognition, and T cell function. Studies have shown that small modifications to the CAR can have significant impact on the therapeutic outcome. Therefore, it key to have a comprehensive library to interrogate as each CAR construct needs intensive testing. 

Twist has developed a technology to build CAR libraries in a manner that seamlessly shuffles variants of each domain via scarless assembly process. 

当サービスの流れ

different sequence

Figure1: Each of these domains can consist of several different sequence variants. These domains are synthesized as genes which Twist combines together in a through scarless assembly process to generate a highly diverse library for scaffold optimization. This technology enables the discovery of unique domain combinations that reveal novel functionalities.

Chimeric Antigen Receptor (CAR) Discovery and Optimization Workflow

CAR の足場の各ドメインは、それぞれ独立して、また相乗的に CAR の機能に影響を与えます。CAR Libraries serve as a tool to fine-tune each of the modules to uncover codependencies and better understand the impact on T-cell specificity, antigen recognition and T-cell function.

Partner with Twist at any stage of your discovery workflow! 

  • Leverage both in vivo and in vitro workflows for binder discovery and optimization
  • Leverage our synthetic libraries for scaffold optimization and validation

T cell receptor

多様性に富むライブラリ
多様性に富むライブラリ
各コンビナトリアル・アセンブリは、最大 10,000 個の二本鎖DNA断片を組み合わせて構成可能
Customize Your CAR Library
Customize Your CAR Library
User defined combinatorial variants across hinge, transmembrane, and signaling domains
高品質
高品質
平均値の 10 倍以内に 90% 超の変異体が存在する、すべて NGS で検証済みのライブラリ
ハイスループット
ハイスループット
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
フレキシブル
フレキシブル
配列中の複数のエレメントにわたって多様性を持つよう、大きなスケールで設計されたライブラリ
ご不明な点はございますか?

より詳細な情報が必要な場合はお気軽にご連絡ください。当社におまかせください。

お問い合わせ

ご注文

Fill out our library's submission form and send it to [email protected] for design review. Once the design review is complete, we will provide you with a quote. The price and turnaround time of your combinatorial assembly libraries will vary per project.

 

Fill out our library's submission form and send it to [email protected] for design review. Once the design review is complete, we will provide you with a quote. The price and turnaround time of your combinatorial assembly libraries will vary per project.

 

資料
バイアスや不要なモチーフを除去
バイアスや不要なモチーフを除去
バイアスや不要なモチーフを除去
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