Clonal Genes

In as fast as four days*, we ship 100% accurate cloned genes prepped to your specification, ready to be dropped into your downstream workflow.

  • Turnaround from 4 business days
  • Starting price $80 / construct
  • Length 0.3 kb – 7.0 kb
  • Scales 1 µg – 1 mg
  • Acceptance rate >99.5%
  • Quality control NGS verified

パフォーマンスデータ

収量

Get reliable yields at every prep scale, backed by fluorometric quantification.

Items meeting advertised minimum yield — Micro 98.6%, Mini 98.2%, Midi 99.9%, Maxi 99.1%.

納期

Keep projects moving with predictable timelines on every order, regardless of scale, complexity or sequence length.

Microprep turnaround time — Express: 6.1 business days average, 5 median; Standard: 9.4 average, 9 median.

Success rate

Every DNA order is routine and reliable, with 98.4% manufacturing success on low and medium complexity sequences. Genes only leave the factory when NGS verified as a 100% match to your desired sequence.

Clonal gene manufacturing success rate — 98.4% Low/Medium complexity, 94.7% High complexity shipped.
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価格

$1,700.00 – $2,550.00

Turnaround time (business days)

4 – 10 (Low/Medium complexity)

10 – 15 (High complexity)

Estimates shown include variations in sequence complexity and speed of service (Standard or Express).

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Input your sequences to check their manufacturability and compare pricing options.

Check your sequences

ベクター

Use our vectors or onboard your own

Can’t find a vector in our catalog? Onboard your vector once. Use it forever.

  • 65+ vectors available in our vector catalog

    Available in your Twist account for immediate use.

  • Onboard your own vectors seamlessly online

    Your vectors will be available on your Twist account to use on every future order. Learn more about vector onboarding.

  • Your IP stays yours

    Your vector is tied to you. We store it and only use it for your requests.

  • Share your vectors with collaborators

    On your request, our support team can make your vectors available to other Twist User accounts at no extra cost.

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製品仕様

Complete product details

SpecificationValue詳細内容
Product format Circular dsDNA
鎖長 0.3 kb – 7.0 kb
Complexity 低 | 中 | 高

A sequence’s complexity score is based on the combined presence, frequency, and length of various features within the design of a sequence. Impacts the ease and speed of production.

High complexity sequences are produced though a series of specialized manufacturing workflows. Supported features include:

  • Short tandem repeats: 3–9bp repeats in up to 100bp stretches
  • Long repeats: repeats up to 200bp
  • Inverted (palindromic) repeats: up to 100bp
  • Localized GC extremes: 10–90% within a 50bp window
  • Sequence-wide GC extremes: 25–75%
  • Stable secondary structures: hairpins, G-quadruplexes
コドン最適化 100s of target organisms Large language model-driven for protein expression.

SpecificationValue詳細内容
Express service (Low/Medium complexity) Add-on 4 – 7 business days* At default scale (Micro). Larger scale preps may increase turnaround time.
Standard service (Low/Medium complexity) 10 business days* At default scale (Micro). Larger scale preps may increase turnaround time.
Standard service (High complexity) 15 business days* At default scale (Micro). Larger scale preps may increase turnaround time.
ベクター
  • 65+ catalog vectors
  • Unlimited custom vectors

Includes cloning, expression, and IVT vectors. View our catalog

One-time onboarding for new custom vectors with unlimited use thereafter.

Custom vector onboarding Add-on 10 営業日 Learn about custom vector onboarding
Synthesis platform Silicon chip (proprietary)
Synthesis chemistry Phosphoramidite (proprietary)
二酸化炭素排出量 ~18 kg CO2e per gene Reagent and energy consumption are 0.16% that of contemporary gene synthesis methods, due to our use of miniaturized silicon chip chemistry.
Animal-origin-free manufacturing Add-on AOF, BSE/TSE Animal origin free certification is included with your order.

SpecificationValue詳細内容
Prep scalesAdd-on

Default:

  • Micro: 1 – 4 µg

Add ons:

  • Mini: 4 – 10 µg
  • Midi: 10 – 100 µg
  • Maxi: 100 µg – 1 mg
Midi and Maxi scale: +3 営業日
Target yieldAdd-on Minimum or exact yield Available at Mini scale.
Purity gradeAdd-on
  • Molecular grade
  • Transfection grade (<10 EU/µg)
  • Endotoxin-free (<0.1 EU/µg)
  • Micro: molecular grade (included)
  • Mini: molecular grade (included)
  • Midi: transfection grade (included) | endotoxin-free
  • Maxi: transfection grade (included) | endotoxin-free
Concentration normalizationAdd-on 0.25 – 1.0 mg/mL Available at Midi and Maxi scale.
Glycerol stockAdd-on Available for all orders
Delivery formatAdd-on Dried down or resuspended
  • Lyophilized
  • 溶出バッファー
  • TE buffer
  • TE buffer low EDTA
  • Nuclease-free water
Container
  • 2 mL tube
  • 96-well plate (regular/deep well)
  • 384-well plate
  • 1.4 mL Micronic tube
  • 4 mL Micronic tube
Micronic tubes and deep well plates available at Midi and Maxi scales.
Shipment conditions Ambient or frozen on dry ice
  • Ambient: Dried down DNA
  • Frozen: Resuspended DNA, Glycerol stocks

Every Clonal Gene we ship includes a Certificate of Analysis (COA) that contains the following information.

SpecificationValue詳細内容
Yield measurementFluorometric quantification
Sequence identity100% match
Sequencing methodNGS (long and/or short read)
Poly-A analysisAdd-onNGS

Did you know Twist does antibodies too?

Your Twist order doesn't have to stop at DNA. We offer high-throughput IgG production and comprehensive characterization integrated into one workflow.

抗体発現サービスを確認する

私たちがお手伝いします

Twist のテクニカルサポートチームは、お客様が大変困難な研究課題に取り組むお手伝いをいたします。If you have any questions about your project, our scientists are here to find the answer.

研究用途に限ります。

診断目的には使用できません。

*この日数は当社施設内での通常処理時間であり、配送業者へ引き渡すまでの目安です。Gene Fragments are shipped in 2-4 business days. Express Genesのご注文の場合、4~7営業日で出荷いたします。Clonal Genesの平均納期は10営業日です。Our target completion timeline for clonal genes scored as complex is 15 business days. Turnaround time for Clonal Genes is subject to change based on customizations and sequence complexity. New vector onboarding for Clonal Genes will add additional turnaround time. Orders placed outside of the US will incur additional delivery time.

†This is for illustrative purposes and the stated benefits, support, benefit calculations and other results are estimates only, based on input from a limited number of existing Twist customers and various assumptions made by the potential customers. This estimate does not constitute a proposal or quote.

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