Gene Fragments

The simple, fast, most cost-effective way to get linear dsDNA. Our industry-leading synthesis quality means less colony screening to find the perfect clone.

  • Turnaround from 2-4 business days
  • Starting price 7¢ (USD) per bp
  • Length 0.3 kb – 5.0 kb
  • Yield 100 ng – 1 µg
  • Error rate 1:7,500

パフォーマンスデータ

エラー率

他社製品と一線を画す、超低エラー率の Twist DNA 合成技術

Items meeting advertised minimum yield — Micro 98.6%, Mini 98.2%, Midi 99.9%, Maxi 99.1%.

Cloning efficiency

Get a higher percentage of perfect clones with Twist Gene Fragments, reducing your time screening colonies.

Microprep turnaround time — Express: 6.1 business days average, 5 median; Standard: 9.4 average, 9 median.

Colony picking calculator

Pick fewer colonies

Our industry-leading fragment synthesis error rate means you need to pick fewer colonies to find the perfect clone. Estimate how many colonies you need to pick here*.

How long is your sequence?
2 – 3 colonies
Based on your sequence length, you should pick 2 – 3 colonies to have a 95% chance to find the perfect clone.
Gene Fragments

*Colony numbers vary depending on sequence complexity, purity, toxicity, and cloning method. In some cases, picking 2–3 times more colonies than estimated here may be necessary.

Price estimator

Get an instant estimate

Sequences

Estimate

価格

$1,050.00

納期

2 – 4 business days

Get a more accurate estimate

Input your sequences to check their manufacturability and compare pricing options.

Check your sequences

製品仕様

Complete product details

Specification Value 詳細内容
Product format Linear dsDNA
鎖長 0.3 kb ~ 5.0 kb
Complexity 低 | 中

A sequence's complexity score is based on the combined presence, frequency, and length of various features within the design of a sequence. Impacts the ease and speed of production.

High complexity sequences are available as Clonal Genes.

コドン最適化 Large language model-based Available for protein expression in hundreds of target organisms.
Amplification adapters Add-on
  • アダプター付き
  • Without adapters

Available to be appended to the 5' and 3' ends of all sequences, allowing for single primer set amplification.

5’– CAATCCGCCCTCACTACAACCG-<insert sequence>-CTACTCTGGCGTCGATGAGGGA – 3’

Specification Value 詳細内容
納期 2 – 4 business days*
収量 100 ng ~ 1 µg
Container 2 mL tube, 96-well plate or
384-well plate
Concentration normalization Add-on 250 ng/fragment
Delivery format Add-on Dried down or resuspended
  • Lyophilized
  • 溶出バッファー
  • TE buffer
  • TE buffer low EDTA
  • Nuclease-free water
Shipment conditions Ambient or frozen on dry ice
  • Ambient: dried down DNA
  • Frozen: 再懸濁させた DNA

Every Gene Fragment we ship includes a Certificate of Analysis (COA) that contains the following information.

Specification Value 詳細内容
Yield measurement Fluorometric quantification
Length verification Capilliary electrophoresis

Did you know Twist does antibodies too?

Your Twist order doesn't have to stop at DNA. We offer high-throughput IgG production and comprehensive characterization integrated into one workflow.

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私たちがお手伝いします

Twist のテクニカルサポートチームは、お客様が大変困難な研究課題に取り組むお手伝いをいたします。If you have any questions about your project, our scientists are here to find the answer.

研究用途に限ります。

診断目的には使用できません。

*この日数は当社施設内での通常処理時間であり、配送業者へ引き渡すまでの目安です。Gene Fragments are shipped in 2-4 business days. Orders placed outside of the US will incur additional delivery time.

†This is for illustrative purposes and the stated benefits, support, benefit calculations and other results are estimates only, based on input from a limited number of existing Twist customers and various assumptions made by the potential customers. This estimate does not constitute a proposal or quote.

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