エラー率
他社製品と一線を画す、超低エラー率の Twist DNA 合成技術
In this study, Twist Gene Fragments have an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over IDT eBlocks™ and gBlocks™ Gene Fragments.
The simple, fast, most cost-effective way to get linear dsDNA. Our industry-leading synthesis quality means less colony screening to find the perfect clone.
他社製品と一線を画す、超低エラー率の Twist DNA 合成技術
In this study, Twist Gene Fragments have an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over IDT eBlocks™ and gBlocks™ Gene Fragments.
Get a higher percentage of perfect clones with Twist Gene Fragments, reducing your time screening colonies.
In this study of 63 unique sequences, our low error rate meant Twist Gene Fragments consistently yielded the highest percentage of perfect clones when compared to IDT eBlocks™ and gBlocks™ Gene Fragments. Cloning efficiency is a crucial factor for determining sequencing costs, which can add up when screening colonies.
Colony picking calculator
Our industry-leading fragment synthesis error rate means you need to pick fewer colonies to find the perfect clone. Estimate how many colonies you need to pick here*.
*Colony numbers vary depending on sequence complexity, purity, toxicity, and cloning method. In some cases, picking 2–3 times more colonies than estimated here may be necessary.
Price estimator
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Input your sequences to check their manufacturability and compare pricing options.
製品仕様
| Specification | Value | 詳細内容 |
|---|---|---|
| Product format | Linear dsDNA | |
| 鎖長 | 0.3 kb ~ 5.0 kb | |
| Complexity | 低 | 中 |
A sequence's complexity score is based on the combined presence, frequency, and length of various features within the design of a sequence. Impacts the ease and speed of production. High complexity sequences are available as Clonal Genes. |
| コドン最適化 | Large language model-based | Available for protein expression in hundreds of target organisms. |
| Amplification adapters Add-on |
|
Available to be appended to the 5' and 3' ends of all sequences, allowing for single primer set amplification. 5’– CAATCCGCCCTCACTACAACCG-<insert sequence>-CTACTCTGGCGTCGATGAGGGA – 3’ |
| Specification | Value | 詳細内容 |
|---|---|---|
| 納期 | 2 – 4 business days* | |
| 収量 | 100 ng ~ 1 µg | |
| Container | 2 mL tube, 96-well plate or 384-well plate |
|
| Concentration normalization Add-on | 250 ng/fragment | |
| Delivery format Add-on | Dried down or resuspended |
|
| Shipment conditions | Ambient or frozen on dry ice |
|
Every Gene Fragment we ship includes a Certificate of Analysis (COA) that contains the following information.
| Specification | Value | 詳細内容 |
|---|---|---|
| Yield measurement | Fluorometric quantification | |
| Length verification | Capilliary electrophoresis |
Your Twist order doesn't have to stop at DNA. We offer high-throughput IgG production and comprehensive characterization integrated into one workflow.
Customers often pair Twist Genes with the following products
Twist のテクニカルサポートチームは、お客様が大変困難な研究課題に取り組むお手伝いをいたします。If you have any questions about your project, our scientists are here to find the answer.
研究用途に限ります。
診断目的には使用できません。
*この日数は当社施設内での通常処理時間であり、配送業者へ引き渡すまでの目安です。Gene Fragments are shipped in 2-4 business days. Orders placed outside of the US will incur additional delivery time.
†This is for illustrative purposes and the stated benefits, support, benefit calculations and other results are estimates only, based on input from a limited number of existing Twist customers and various assumptions made by the potential customers. This estimate does not constitute a proposal or quote.
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