This Twist RNA Library Preparation Protocol details all the necessary steps to build sequencing libraries for target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. The steps include converting RNA to double-stranded cDNA and preparing cDNA libraries using the Twist Universal Adapter System. Twist Universal Adapter Systemは、TwistユニバーサルアダプターとTwist Unique Dual Indexed（UDI）プライマーで構成されています。本キットは、 Twist Universal Adapters および Twist HT UDI Primers で構成されており、 Twist HT Universal Adapter System に対応しています。This protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.