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Heteroduplexes affect accurate library size determination without impacting targeted sequencing performance

Genomic DNA (gDNA) libraries are prepared for next-generation sequencing (NGS) by fragmenting the gDNA and ligating adapter duplexes to the ends of the fragments. To ensure efficient downstream target enrichment and sequencing, the libraries should contain a controlled and narrow distribution of gDNA fragment sizes (Head et al. 2014).