Genes
New Features For Clonal Genes
We’ve enhanced our Clonal Genes products with additional plasmid prep sizes and other options including normalization, endotoxin-free DNA, and a variety of tube and plate options to meet your research needs.
Performance At Scale
Our silicon-based, high-precision DNA synthesis platform results in higher quality genes and significantly increased throughput and scalability.
We offer both Clonal Genes and Gene Fragments for your research needs:
Clonal Genes are synthesized DNA cloned into a plasmid vector and NGS sequence verified.
Gene Fragments are linear synthesized DNA sequences for direct cloning or larger gene assembly.
Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery.
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated
antibody development process that aims to provide access
to treatments within 60 days.
New Features For Clonal Genes
We’ve enhanced our Clonal Genes products with additional plasmid prep sizes and other options including normalization, endotoxin-free DNA, and a variety of tube and plate options to meet your research needs.
Performance At Scale
Our silicon-based, high-precision DNA synthesis platform results in higher quality genes and significantly increased throughput and scalability.
We offer both Clonal Genes and Gene Fragments for your research needs:
Clonal Genes are synthesized DNA cloned into a plasmid vector and NGS sequence verified.
Gene Fragments are linear synthesized DNA sequences for direct cloning or larger gene assembly.
Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery.
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated
antibody development process that aims to provide access
to treatments within 60 days.
Clonal Genes
Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.
A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.
Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.
Clonal Genes
Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.
A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.
Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.
Gene Fragments
Synthetic Gene Fragments are an inexpensive, rapid, and efficient way to build the genes you need for your research. Gene Fragments from Twist Bioscience improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.
Accurate fragment synthesis accelerates discovery. Twist Bioscience Gene Fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones.
Best In Class Performance
Twist’s DNA synthesis technology exhibits extremely low error rates. This level of precision makes larger gene assembly more reliable and easier to achieve.
In a direct comparison with competitor products Twist outperformed with the lowest error rate. The same sequences were ordered from Twist and Integrated DNA Technologies, Inc. (IDT) for genes of various lengths, the genes were then cloned and transformed, colonies were picked and sequenced. The data represent the average error rate across the given sequences in each set.
As seen in the figure below, Twist Gene Fragments had a nearly 2-fold improvement in error rate over IDT gBlocks across the entire size range and a 1.7-fold improvement over eBlocks across the range of 300 - 800 bp, with a 1:5,367 bp and 1:6,253 bp error rate respectively. Low error rates translate into fewer colonies that need to be picked and screened to find your perfect gene saving you time and money.
Affordable Gene Synthesis
Accurate fragment synthesis accelerates discovery. Twist Bioscience Gene Fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones. Twist’s lower overall error rate results in more perfect sequences. The performance and scale of Twists Gene synthesis translate into a lower price and less effort per gene. The following figure illustrates the cost per gene ordered as a Gene Fragment from Twist Bioscience. Buying ready-to-use genes is easier, faster, and less expensive than you thought.
Gene Fragments now available with or without Twist Adapters.
Gene Fragments
Synthetic Gene Fragments are an inexpensive, rapid, and efficient way to build the genes you need for your research. Gene Fragments from Twist Bioscience improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.
Accurate fragment synthesis accelerates discovery. Twist Bioscience Gene Fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones.
Best In Class Performance
Twist’s DNA synthesis technology exhibits extremely low error rates. This level of precision makes larger gene assembly more reliable and easier to achieve.
In a direct comparison with competitor products Twist outperformed with the lowest error rate. The same sequences were ordered from Twist and Integrated DNA Technologies, Inc. (IDT) for genes of various lengths, the genes were then cloned and transformed, colonies were picked and sequenced. The data represent the average error rate across the given sequences in each set.
As seen in the figure below, Twist Gene Fragments had a nearly 2-fold improvement in error rate over IDT gBlocks across the entire size range and a 1.7-fold improvement over eBlocks across the range of 300 - 800 bp, with a 1:5,367 bp and 1:6,253 bp error rate respectively. Low error rates translate into fewer colonies that need to be picked and screened to find your perfect gene saving you time and money.
Affordable Gene Synthesis
Accurate fragment synthesis accelerates discovery. Twist Bioscience Gene Fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones. Twist’s lower overall error rate results in more perfect sequences. The performance and scale of Twists Gene synthesis translate into a lower price and less effort per gene. The following figure illustrates the cost per gene ordered as a Gene Fragment from Twist Bioscience. Buying ready-to-use genes is easier, faster, and less expensive than you thought.
Gene Fragments now available with or without Twist Adapters.
Product Sheet
Clonal Genes Product Sheet
Twist Bioscience is transforming gene synthesis, a process at the core of synthetic and molecular biology. Our silicon-based DNA writing platform significantly increases gene synthesis throughput and scalability, while also reducing turnaround time and price per base.
Product Sheet
Gene Fragments Product Sheet
Twist Bioscience gene fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs。
Case Study
Build a better DNA Assembly Line
Arzeda leverages technology from Labcyte, TeselaGen and Twist Bioscience to accelerate their synthetic biology workflow.
Application Note
Pathway Engineering Through 5 kb Gene Synthesis
A rapid design-build-test cycle is vital to metabolic engineering applications in which researchers re-program microorganisms to produce non-native molecules of interest. To produce these molecules, researchers typically add one gene or an entire pathway of genes into a host organism. These genes encode proteins or enzymes that, when expressed in a microbial host, convert a common substrate into a molecule of interest.
Case Study
Speeding Up Antibody Discovery for Infectious Disease
Tasked with an ambitious goal from DARPA to develop a rapid response to help medical workers fight viral diseases in the field, Vanderbilt University Medical Center has already reduced the time to develop antibodies significantly. High-throughput, synthetic genes from Twist Bioscience have allowed the lab to expedite this process.
Webinar
Amyris discusses Next-Generation Platforms for Strain Optimization
Watch this webinar showing how Twist Genes are enabling this innovative company with their microbial engineering efforts.https://www.twistbioscience.com/genes_bioengineeringwebinar_sunilchandran
Product Sheet
Twist Vectors Product Sheet
Twist Bioscience synthesizes high-quality, NGS-verified custom genes at a… cost and scale that are otherwise unavailable. For researchers wanting to replicate their synthetic genes or use them in expression studies, Twist provides the option of delivery in a diverse selection of cloning and expression vectors through a convenient online ordering platform.
Application Note
Twist Tips for Genes: How to Design Your Gene
Twist Bioscience uses a silicon-based DNA synthesis platform to generate… high-quality synthetic DNA. Designing and ordering genes from Twist Bioscience is simple when using our online ordering platform. This document provides tips for optimizing and customizing Twist Clonal Genes and Gene Fragments for a variety of applications
Application Note
Twist Tips for Genes - Meeting Minimum Length Requirements for Genes in Custom Vectors
When you submit a sequence for synthesis, the scoring algorithm checks the sequence to determine whether it can be synthesized, and one of the first things it checks is whether the sequence meets the minimum length requirement of 300 bp. Use this document to help you design your Genes when they do not meet the minimum length requirement.
Application Note
From Design to Protein: Cell-Free Protein Expression Using Twist Gene Fragments
Application Note
Gene Fragments Cloning Guidelines
Twist Bioscience’s Gene Fragments are double-stranded DNA, 300 to… 1800 base pairs in length. These high-quality fragments come with unique 5’ and 3’ adapter sequences.
Product Sheet
Clonal Genes Product Sheet
Twist Bioscience is transforming gene synthesis, a process at the core of synthetic and molecular biology. Our silicon-based DNA writing platform significantly increases gene synthesis throughput and scalability, while also reducing turnaround time and price per base.
Product Sheet
Gene Fragments Product Sheet
Twist Bioscience gene fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs。
Case Study
Build a better DNA Assembly Line
Arzeda leverages technology from Labcyte, TeselaGen and Twist Bioscience to accelerate their synthetic biology workflow.
Application Note
Pathway Engineering Through 5 kb Gene Synthesis
A rapid design-build-test cycle is vital to metabolic engineering applications in which researchers re-program microorganisms to produce non-native molecules of interest. To produce these molecules, researchers typically add one gene or an entire pathway of genes into a host organism. These genes encode proteins or enzymes that, when expressed in a microbial host, convert a common substrate into a molecule of interest.
Case Study
Speeding Up Antibody Discovery for Infectious Disease
Tasked with an ambitious goal from DARPA to develop a rapid response to help medical workers fight viral diseases in the field, Vanderbilt University Medical Center has already reduced the time to develop antibodies significantly. High-throughput, synthetic genes from Twist Bioscience have allowed the lab to expedite this process.
Webinar
Amyris discusses Next-Generation Platforms for Strain Optimization
Watch this webinar showing how Twist Genes are enabling this innovative company with their microbial engineering efforts.https://www.twistbioscience.com/genes_bioengineeringwebinar_sunilchandran
Product Sheet
Twist Vectors Product Sheet
Twist Bioscience synthesizes high-quality, NGS-verified custom genes at a… cost and scale that are otherwise unavailable. For researchers wanting to replicate their synthetic genes or use them in expression studies, Twist provides the option of delivery in a diverse selection of cloning and expression vectors through a convenient online ordering platform.
Application Note
Twist Tips for Genes: How to Design Your Gene
Twist Bioscience uses a silicon-based DNA synthesis platform to generate… high-quality synthetic DNA. Designing and ordering genes from Twist Bioscience is simple when using our online ordering platform. This document provides tips for optimizing and customizing Twist Clonal Genes and Gene Fragments for a variety of applications
Application Note
Twist Tips for Genes - Meeting Minimum Length Requirements for Genes in Custom Vectors
When you submit a sequence for synthesis, the scoring algorithm checks the sequence to determine whether it can be synthesized, and one of the first things it checks is whether the sequence meets the minimum length requirement of 300 bp. Use this document to help you design your Genes when they do not meet the minimum length requirement.
Application Note
From Design to Protein: Cell-Free Protein Expression Using Twist Gene Fragments
Application Note
Gene Fragments Cloning Guidelines
Twist Bioscience’s Gene Fragments are double-stranded DNA, 300 to… 1800 base pairs in length. These high-quality fragments come with unique 5’ and 3’ adapter sequences.
Article
Machine Learning-Guided Prediction of Antigen-Reactive In Silico Clonotypes Based on Changes in Clonal Abundance through Bio-Panning
Article
The LRRC8A:C Heteromeric Channel Is a cGAMP Transporter and the Dominant cGAMP Importer in Human Vasculature Cells
Article
De novo protein design enables precise induction of functional antibodies in vivo
Article
In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription
Article
Machine Learning-Guided Prediction of Antigen-Reactive In Silico Clonotypes Based on Changes in Clonal Abundance through Bio-Panning
Article
The LRRC8A:C Heteromeric Channel Is a cGAMP Transporter and the Dominant cGAMP Importer in Human Vasculature Cells
Article
De novo protein design enables precise induction of functional antibodies in vivo
Article
In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription
Genes Calculator
COMPARED TO***
**Cost savings based on comparison to public list prices for standard reagents. Time savings based on Twist's internal testing of typical times for standard manual cloning.
***Savings based on comparison to competitor’s publicly-listed pricing as of May 15, 2019. Turn-around times between Twist and competitors may vary.
