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App Note

Sequencing the Unsequenceable; Formalin-Fixed, Paraffin-Embedded (FFPE) Sample Processing for Successful Target Enrichment

Library construction for Next Generation Sequencing (NGS)
using formalin-fixed paraffin-embedded (FFPE) samples offers unique challenges in acquiring high-quality sequencing data due to wide distribution of sample quality. Differences in formalin fixation methods, storage conditions, and age lead to crosslinked and/or degraded nucleic acid and inconsistent extraction yields.

Therefore, FFPE extraction and library construction methods must be carefully considered for target enrichment applications.

In collaboration, Covaris and Twist Bioscience demonstrate a complete library generates read to-sequence multiplexed libraries directly from FFPE tissue of various qualities.