The Power of Silicon-based DNA Synthesis
Twist Bioscience’s high precision, silicon-based high-throughput gene synthesis platform enables us to produce high quality gene fragments starting at 7¢ (USD) per bp and NGS-verified sequence perfect clonal genes starting at 9¢ (USD) per bp. What does this mean for you? Rapid and accurate DNA synthesis at the scale you need for the price you want.
What Do We Offer?
Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery. Still not convinced? Talk to one of our resident experts!
Gene Fragments
Linear synthesized DNA sequences for direct cloning or larger gene assembly.
Express Genes and Clonal Genes
Synthesized DNA cloned into a plasmid vector and NGS sequence verified.
How It Works
Normally, genes are synthesized with one gene per 96 well plate. Here at Twist, we have perfected gene synthesis and with our proprietary platform is able to synthesize 9600 genes per one silicon-based chip in a single run. This means we can produce 1 gene or 100,000 genes in parallel, in the same amount of time.
Get your genes faster than ever without compromising sequence integrity or quality with Twist Bioscience. Here’s what to do next:
All you have to do is design your custom sequence and our high quality gene synthesis platform will do the rest.
For Research Use Only. Not for Diagnostic Procedures.
* Calculated Twist internal data using Dr. Oligo benchmark January 2021 and validated by SRI Quality System Registrar and Silinnov Consulting in 2023
* This tool is therefore provided strictly for illustrative purposes and the stated benefits, support, benefit calculations and other results are estimates only, and this estimate does not constitute a proposal or quote.
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Testimonials
Featured Resources
Application Note
Ordering online just got easier. Watch our “How to” video to see how to order genes all online from our eCommerce platform.
The Power of Silicon-based DNA Synthesis
Twist Bioscience’s high precision, silicon-based high-throughput gene synthesis platform enables us to produce high quality gene fragments starting at 7¢ (USD) per bp and NGS-verified sequence perfect clonal genes starting at 9¢ (USD) per bp. What does this mean for you? Rapid and accurate DNA synthesis at the scale you need for the price you want.
What Do We Offer?
Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery. Still not convinced? Talk to one of our resident experts!
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Gene Fragments
Linear synthesized DNA sequences for direct cloning or larger gene assembly.
Express Genes and Clonal Genes
Synthesized DNA cloned into a plasmid vector and NGS sequence verified.
How It Works
Normally, genes are synthesized with one gene per 96 well plate. Here at Twist, we have perfected gene synthesis and with our proprietary platform is able to synthesize 9600 genes per one silicon-based chip in a single run. This means we can produce 1 gene or 100,000 genes in parallel, in the same amount of time.
Get your genes faster than ever without compromising sequence integrity or quality with Twist Bioscience. Here’s what to do next:
All you have to do is design your custom sequence and our high quality gene synthesis platform will do the rest.
For Research Use Only. Not for Diagnostic Procedures.
* Calculated Twist internal data using Dr. Oligo benchmark January 2021 and validated by SRI Quality System Registrar and Silinnov Consulting in 2023
* This tool is therefore provided strictly for illustrative purposes and the stated benefits, support, benefit calculations and other results are estimates only, and this estimate does not constitute a proposal or quote.
Testimonials
Featured Resources
Ordering online just got easier. Watch our “How to” video to see how to order genes all online from our eCommerce platform.
Sequence Perfect Clonal Genes
A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.
*Terms and Conditions: Eligible Express Genes ship in 4-7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Average turnaround time for Clonal Genes is 10 business days. New vector onboarding for both Express Genes and Clonal Genes will add additional time. Pricing calculator only refers to Gene Fragments and Clonal Genes offering, not inclusive of Express Genes service pricing.
For Research Use Only. Not for Diagnostic Procedures.
FAQ
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Join Dr. Rhys Grinter of the University of Melbourne to learn how AI/ML and Twist Clonal Genes enable rapid design and screening of protein binders that block bacterial heme-piracy.
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Sequence Perfect Clonal Genes
A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.
*Terms and Conditions: Eligible Express Genes ship in 4-7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Average turnaround time for Clonal Genes is 10 business days. New vector onboarding for both Express Genes and Clonal Genes will add additional time. Pricing calculator only refers to Gene Fragments and Clonal Genes offering, not inclusive of Express Genes service pricing.
For Research Use Only. Not for Diagnostic Procedures.
FAQ
Join Dr. Rhys Grinter of the University of Melbourne to learn how AI/ML and Twist Clonal Genes enable rapid design and screening of protein binders that block bacterial heme-piracy.
Twist’s DNA synthesis technology outperforms the competition with exceptionally low error rates.
In a direct comparison of Gene Fragment products, Twist consistently showed the lowest error rate. The graph to the left represents the average error rate for Gene sequences ordered from Twist and Integrated DNA Technologies, Inc. (IDT). The results show Twist Gene Fragments have greater sequence accuracy over eBlocks and gBlocks with an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over gBlocks.
More perfect Genes from Twist
Twist Gene Fragments consistently yield the highest percentage of perfect clones which saves you time and money in your research. The graph to the left shows a direct comparison of the percentage of sequence perfect clones across a wide variety of gene lengths and sequences for 3 different gene products. Data was generated using a set of 63 sequences with a wide variety of gene lengths to reflect the diversity of gene lengths required for various real-world synthetic biology applications.
*This timeframe refers to the typical processing and handling time within our facilities before your order is handed over the shipping carrier.
FAQ
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Dr. Binnian of Resurrect Bio shares how her team uses AI-driven gene design and Twist Genes to tackle plant immunity challenges, rapidly screening thousands of sequences from in silico prediction to in planta validation.
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Twist’s DNA synthesis technology outperforms the competition with exceptionally low error rates.
In a direct comparison of Gene Fragment products, Twist consistently showed the lowest error rate. The graph to the left represents the average error rate for Gene sequences ordered from Twist and Integrated DNA Technologies, Inc. (IDT). The results show Twist Gene Fragments have greater sequence accuracy over eBlocks and gBlocks with an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over gBlocks.
More perfect Genes from Twist
Twist Gene Fragments consistently yield the highest percentage of perfect clones which saves you time and money in your research. The graph to the left shows a direct comparison of the percentage of sequence perfect clones across a wide variety of gene lengths and sequences for 3 different gene products. Data was generated using a set of 63 sequences with a wide variety of gene lengths to reflect the diversity of gene lengths required for various real-world synthetic biology applications.
Dr. Binnian of Resurrect Bio shares how her team uses AI-driven gene design and Twist Genes to tackle plant immunity challenges, rapidly screening thousands of sequences from in silico prediction to in planta validation.
*This timeframe refers to the typical processing and handling time within our facilities before your order is handed over the shipping carrier.
FAQ
Product Sheet
Case Study
Webinar
Protocol
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Product Sheet
克隆基因产品彩页
Twist Bioscience 正在改变合成生物学和分子生物学的核心过程 — 基因合成。我们的硅基 DNA 编写平台显著提高了基因合成通量和可扩展性，同时减少了每个碱基的交付时间和价格。
Case Study
建立更好的 DNA 组装流程
Arzeda 利用来自 Labcyte、TeselaGen 和 Twist Bioscience 的技术加速其合成生物学的工作流程。
/resource/building-better-dna-assembly-line
通过 5 kb 基因合成的通路工程
在代谢工程应用中，研究人员可对微生物进行重新编程，生成非天然的目标分子，而快速的设计-合成-测试循环对于这些应用极为重要。为了生成这些分子，研究人员通常会向宿主生物添加一个基因或整条通路的基因。这些基因能编码蛋白或酶，当这些蛋白或酶在微生物宿主中表达时，可将常见的底物转化为目标分子。
Case Study
加速传染病的抗体发现
范德堡大学医学中心接到了来自 DARPA 的任务，即开发一种快速反应机制来帮助医务工作者对抗相关领域的病毒性疾病，该中心已经大大缩短了开发抗体的时间。Twist Bioscience 的高通量合成基因帮助该实验室加快了这个过程。
Twist 基因技巧：如何设计基因
Twist Bioscience 使用硅基 DNA 合成平台来生产高质量的合成 DNA。使用我们的在线订购平台即可从 Twist Bioscience 轻松设计和订购基因。本文档提供了针对各种应用优化和定制 Twist 克隆基因和基因片段的技巧。
Twist 基因技巧 — 满足定制载体中基因的最小长度要求
提交序列进行合成时，评分算法会检查序列，确定它能否被合成，它首先检查的是序列是否满足 300 bp 的最小长度要求。当您的基因不符合最小长度要求时，请使用该文档来帮助您设计基因。
从基因设计到蛋白表达：使用 Twist 基因片段进行无细胞蛋白表达
基因片段克隆指南
Twist Bioscience 的基因片段是300 至 1800 bp的双链 DNA。这些高质量片段具有独特的 5’和 3’接头序列。
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Article
Article
Machine Learning-Guided Prediction of Antigen-Reactive In Silico Clonotypes Based on Changes in Clonal Abundance through Bio-Panning
Article
The LRRC8A:C Heteromeric Channel Is a cGAMP Transporter and the Dominant cGAMP Importer in Human Vasculature Cells
Article
In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Article
Machine Learning-Guided Prediction of Antigen-Reactive In Silico Clonotypes Based on Changes in Clonal Abundance through Bio-Panning
Article
The LRRC8A:C Heteromeric Channel Is a cGAMP Transporter and the Dominant cGAMP Importer in Human Vasculature Cells
Article
In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
General
Clonal Genes
Codon Optimization
Sequence Design
**Comparison to competitor’s publicly listed pricing as of July 12, 2024. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
