Start Customizing Your Genes
Start Customizing Your Genes
Start Customizing Your Genes
OVERVIEW CLONAL FRAGMENT TECHNICAL RESOURCES REFERENCES FAQ
OVERVIEW

The Power of Silicon-based DNA Synthesis

Our silicon-based, high-precision DNA synthesis platform results in higher quality genes and significantly increased throughput and scalability. We offer:

Gene Fragments

Linear synthesized DNA sequences for direct cloning or larger gene assembly.

Learn more

Clonal Genes

Synthesized DNA cloned into a plasmid vector and NGS sequence verified.

Learn more

 clonal genes

Reduce Costs With High Quality

Comparison of total costs for DNA synthesis, cloning, and sequence verification of constructs built with gene fragments. Cloning cost is assumed to be $30 per gene and sequencing cost is assumed to be $5 per clone sequenced. Sequencing more clones of fragments synthesized by the competitor is required to obtain correct sequence with 99% confidence, increasing the total cost for each gene.

High Quality Genes

Twist’s DNA synthesis technology exhibits extremely low error rates, the average error rate is one in 3,000 base pairs. This level of precision makes larger gene assembly more reliable and easier to achieve. See how our high quality DNA can accelerate your timelines here.

Simple Ordering Flexible Offering

Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery.

How It Works

how it works

Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
Twist Gene in Action

  

Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.

Read the Case Study

Asset

 

Testimonials

The combination of price and service allows us to do science that we could not otherwise do
PROFESSOR NICHOLAS HARMER
University of Exeter
The quality has been excellent, and I find that cloning with these genes is successful more often on the first time than with DNA fragments from other companies
DR. AMANDA PORTILLO
Institute of Systems and Synthetic Biology
This is where we came to really like the online ordering portal, that tells you then and there whether Twist can make your gene. We input our sequences and Twist’s algorithms right away said “yes, why not?”. It turned out that for Twist, making these difficult sequences was easy.
DR. PHILIPPE MARLIERE
Institute of Systems and Synthetic Biology
gene video thumbnail

Ordering online just got easier. Watch our “How to” video to see how to order genes all online from our eCommerce platform

The Power of Silicon-based DNA Synthesis

Our silicon-based, high-precision DNA synthesis platform results in higher quality genes and significantly increased throughput and scalability. We offer:

Gene Fragments

Linear synthesized DNA sequences for direct cloning or larger gene assembly.

Learn more

Clonal Genes

Synthesized DNA cloned into a plasmid vector and NGS sequence verified.

Learn more

 clonal genes

Reduce Costs With High Quality

Comparison of total costs for DNA synthesis, cloning, and sequence verification of constructs built with gene fragments. Cloning cost is assumed to be $30 per gene and sequencing cost is assumed to be $5 per clone sequenced. Sequencing more clones of fragments synthesized by the competitor is required to obtain correct sequence with 99% confidence, increasing the total cost for each gene.

High Quality Genes

Twist’s DNA synthesis technology exhibits extremely low error rates, the average error rate is one in 3,000 base pairs. This level of precision makes larger gene assembly more reliable and easier to achieve. See how our high quality DNA can accelerate your timelines here.

Simple Ordering Flexible Offering

Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery.

How It Works

how it works

Twist Gene in Action

  

Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.

Read the Case Study

Asset

 

Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.

Testimonials

The combination of price and service allows us to do science that we could not otherwise do
PROFESSOR NICHOLAS HARMER
University of Exeter
The quality has been excellent, and I find that cloning with these genes is successful more often on the first time than with DNA fragments from other companies
DR. AMANDA PORTILLO
Institute of Systems and Synthetic Biology
This is where we came to really like the online ordering portal, that tells you then and there whether Twist can make your gene. We input our sequences and Twist’s algorithms right away said “yes, why not?”. It turned out that for Twist, making these difficult sequences was easy.
DR. PHILIPPE MARLIERE
Institute of Systems and Synthetic Biology
gene video thumbnail

Ordering online just got easier. Watch our “How to” video to see how to order genes all online from our eCommerce platform

Clonal

Clonal Genes

Twist Bioscience’s platform is capable of synthesizing hundreds of thousands of genes each month to meet all your DNA needs. Our silicon-based platform for DNA synthesis enables us to create at scale, highly precise, sequence perfect clonal genes of various lengths and difficulty that are all NGS verified and delivered on-time when you need them.

 Low Cost – High Quality clonal gene
Low Cost – High Quality
From 9¢ per bp
No hidden sub-cloning or DNA complexity fees
From 10 business days
DNA Your Way clonal gene
DNA Your Way
0.3 – 5 kb genes cloned into a plasmid of your choice
Choose a Twist Catalog Vector or send us yours
4 prep scales (50ng – 2µg | 2µg – 10µg | 10µg – 100µg | 100µg – 1mg)
Normalization and endotoxin free options available
Scalable Synthesis clonal gene
Scalable Synthesis
No order limits
Same turnaround regardless of order size

 Genes clonal genes

Sequence Perfect Genes

A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.

Genes clonal genes

Clonal Genes Success Rate

Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.

FAQ

Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
New Features for Clonal Genes

Get DNA your way with new prep sizes and options.

Get Started

DNA

Clonal Genes

Twist Bioscience’s platform is capable of synthesizing hundreds of thousands of genes each month to meet all your DNA needs. Our silicon-based platform for DNA synthesis enables us to create at scale, highly precise, sequence perfect clonal genes of various lengths and difficulty that are all NGS verified and delivered on-time when you need them.

Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
New Features for Clonal Genes

Get DNA your way with new prep sizes and options.

Get Started

DNA

 Low Cost – High Quality clonal gene
Low Cost – High Quality
From 9¢ per bp
No hidden sub-cloning or DNA complexity fees
From 10 business days
DNA Your Way clonal gene
DNA Your Way
0.3 – 5 kb genes cloned into a plasmid of your choice
Choose a Twist Catalog Vector or send us yours
4 prep scales (50ng – 2µg | 2µg – 10µg | 10µg – 100µg | 100µg – 1mg)
Normalization and endotoxin free options available
Scalable Synthesis clonal gene
Scalable Synthesis
No order limits
Same turnaround regardless of order size

 Genes clonal genes

Sequence Perfect Genes

A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.

Genes clonal genes

Clonal Genes Success Rate

Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.

FAQ

Fragment

Gene Fragments

Synthetic Gene Fragments are an inexpensive, rapid, and efficient way to build the genes you need for your research. Gene Fragments from Twist Bioscience improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs.

Think bigger, design on a grander scale, and accelerate your discoveries.
Order Now or Get the Product Sheet.

Fast and Economical
Fast and Economical
Gene Fragments for assembly and cloning
Compatible with all downstream cloning methods
From 7¢ per bp
From 5 business days
Screen Less Discover More Gene Fragments
Screen Less Discover More
Industry leading error rate
Length: 300 bp – 1800 bp
Yield: 100 ng – 1 µg
No order limit

Twist’s DNA synthesis technology outperforms the competitions with exceptionally low error rates.

In a direct comparison of Gene Fragment products, Twist consistently showed the lowest error rate. The graph to the left represents the average error rate for Gene sequences ordered from Twist and Integrated DNA Technologies, Inc. (IDT). The results show Twist Gene Fragments have greater sequence accuracy over eBlocks and gBlocks with an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over gBlocks.


 

Gene Fragments average error rate

 

More perfect Genes from Twist

Twist Gene Fragments consistently yield the highest percentage of perfect clones which saves you time and money in your research. The graph to the left shows a direct comparison of the percentage of sequence perfect clones across a wide variety of gene lengths and sequences for 3 different gene products. Data was generated using a set of 63 sequences with a wide variety of gene lengths to reflect the diversity of gene lengths required for various real-world synthetic biology applications.

perfect genes from twist

 

FAQ

Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
Twist Gene in Action

Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.

Read the Case Study

pandemic prevention platform

Gene Fragments

Synthetic Gene Fragments are an inexpensive, rapid, and efficient way to build the genes you need for your research. Gene Fragments from Twist Bioscience improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs.

Think bigger, design on a grander scale, and accelerate your discoveries.
Order Now or Get the Product Sheet.

Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
Fast and Economical
Fast and Economical
Gene Fragments for assembly and cloning
Compatible with all downstream cloning methods
From 7¢ per bp
From 5 business days
Screen Less Discover More Gene Fragments
Screen Less Discover More
Industry leading error rate
Length: 300 bp – 1800 bp
Yield: 100 ng – 1 µg
No order limit

Twist’s DNA synthesis technology outperforms the competitions with exceptionally low error rates.

In a direct comparison of Gene Fragment products, Twist consistently showed the lowest error rate. The graph to the left represents the average error rate for Gene sequences ordered from Twist and Integrated DNA Technologies, Inc. (IDT). The results show Twist Gene Fragments have greater sequence accuracy over eBlocks and gBlocks with an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over gBlocks.


 

Gene Fragments average error rate

 

More perfect Genes from Twist

Twist Gene Fragments consistently yield the highest percentage of perfect clones which saves you time and money in your research. The graph to the left shows a direct comparison of the percentage of sequence perfect clones across a wide variety of gene lengths and sequences for 3 different gene products. Data was generated using a set of 63 sequences with a wide variety of gene lengths to reflect the diversity of gene lengths required for various real-world synthetic biology applications.

perfect genes from twist

 

Twist Gene in Action

Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.

Read the Case Study

pandemic prevention platform

FAQ

Technical RESOURCES
Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
REFERENCES
Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
FAQ

General

Clonal Genes

Codon Optimization

Sequence Design

Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.

General

Clonal Genes

Codon Optimization

Sequence Design

Fragments*
Clonal*
0.3kb – 1.8kb
Starting at 7¢ per bp
From 5 business days
No order limits
0.3kb – 5kb
From 10 business days
50ng – 2μg | 2μg – 10μg
10μg – 100μg | 100μg – 1mg
No order limits
Gene Fragment

0.3

0.6

0.9

1.2

1.5

1.8kb
Length
base pairs
Quantity
genes
Please enter a quantity between 1 and 10000.
Vector Options
ESTIMATED TOTAL
$54,000
START DESIGN
Saving With Twist Compared to **
Saving Estimate
30%
$10,000
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.

**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.

Finally, an easy way to order DNA

 

Instantly know if your sequence is a go
Our smart algorithm lets you know in seconds if your sequence can be synthesized or not

Your quote is now just one click away
No more wasted time waiting for surprise quotes. See real-time pricing when you submit your data

Stay in the know from checkout to your door
We update you at every stage of your orders’ progress, giving you comprehensive insight on crucial production details

Intuitive usability meets bulletproof security
Upload your sequences with the comfort of knowing your proprietary data is protected

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