Site Saturation Libraries
Precisely crafted
- Superior precision and diversity compared to random mutagenesis or site directed mutagenesis
- No introduction of stop or unwanted codons
- Precise codon ratio control
- Fast turnaround time
- More cost effective than recombinatorial mutagenesis
Superior Quality
- All Site Saturation Libraries are NGS-verified
- Documentation showing the percentage ratio of amino acid variation at each position is included
- Rigorous quality control and verification of all variants
- Flexible delivery format options to meet your needs
Product Specifications
| Product format | Linear double-stranded DNA |
| Delivery and Yield | All positions in a single tube, 1-2 ug total (additional fee applies) 96 well plate, 1 position in a well, 50 ng each |
| Competitive pricing | $50 per position |
| Minimum Order | 48 variants |
| Turnaround Time | 3-4 weeks, project dependent |
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Combinatorial Libraries
Flexibility and Precision
- Greater flexibility than the degenerate approach
- Suitable for sites with stretches of diversity
- Not sequence dependent; no concerns about over or under representation
- No introduction of stop or unwanted codons
- Precise codon ratio control
- More cost effective compared to traditional combinatorial mutagenesis
Superior Quality
- Combinatorial Libraries with NGS-verification of modified regions
- Sequence variant ratios documented
- Rigorous quality control and verification of all variants
Product Specifications
| Product format | Linear double-stranded DNA |
| Delivery and Yield | All positions in a single tube, up to 1 ug total (length dependent) |
| Competitive pricing | Project dependent, contact for details |
| Turnaround Time | 4-6 weeks, project dependent |
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Spread-Out Low Diversity (SOLD) Libraries
Superior Quality and Accuracy
- <1000 bp gene variant library with diversity scattered across parental sequence
- Cost-effective tool for constructing libraries with diversity >10^8
- High fidelity synthetic construct requiring no template
- Balanced codon usage, avoidance of restriction sites and undesirable mutations
- Precision alternative to NNK, TRIM, error prone PCR, random mutagenesis, and DNA shuffling
Product Application
- Enzyme Evolution
- Enzyme Optimization
- Enzyme Specificity
- Protein/Enzyme Engineering
Product Specifications
| Product format | Linear double-stranded DNA, optional cloning |
| Delivery and Yield | All variants pooled in a single tube, deliver up to 1 ug |
| Competitive pricing | Project dependent, contact for details |
| Turnaround Time | 2-3 weeks, project dependent |
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