Performance at any scale
Our revolutionary silicon-based DNA Synthesis platform is capable of producing over a million unique ssDNA oligos in a single run. Each chip contains Thousands of discrete clusters each containing 121 individually addressable surfaces capable of synthesizing a unique oligo sequence. A representation of the silicon chip technology can be seen below.
Due to the sheer scale of DNA synthesis capability on the chip there are virtually no limitations to the number of unique oligos that can be assembled into a pool. Don't restrict the outcome of your application, order Oligo Pools tailored precisely to your experiment.
More Space for Innovation
Don’t be limited by length. Encode the elements and sequences you need, including promoters, enhancers, guides, and probes all within the expanded architecture. With oligo lengths up to 300nt it’s possible to encode multiple elements in a single design giving you the ability to do more with each oligo.
Screen once, screen right
Unmatched pool uniformity without bias provides more unique targets per screen. This means less effort is required to achieve the data coverage you need, saving time and money.
Be data confident
With the precision and uniformity of Twist Oligo Pools, you can be confident in the quality of your experimental results. Don’t miss a datapoint due to synthesis errors or inefficiencies. Get industry-leading oligo representation and low error rate for oligos of any length up to 300nt, and pools of any size. Leverage these exceptional quality oligo pools at the precise scale and length required for your application.
Tell us what you would like more information on. We’re here to help!
Contact Us
Performance at any scale
Our revolutionary silicon-based DNA Synthesis platform is capable of producing over a million unique ssDNA oligos in a single run. Each chip contains Thousands of discrete clusters each containing 121 individually addressable surfaces capable of synthesizing a unique oligo sequence. A representation of the silicon chip technology can be seen below.
Due to the sheer scale of DNA synthesis capability on the chip there are virtually no limitations to the number of unique oligos that can be assembled into a pool. Don't restrict the outcome of your application, order Oligo Pools tailored precisely to your experiment.
More Space for Innovation
Don’t be limited by length. Encode the elements and sequences you need, including promoters, enhancers, guides, and probes all within the expanded architecture. With oligo lengths up to 300nt it’s possible to encode multiple elements in a single design giving you the ability to do more with each oligo.
Screen once, screen right
Unmatched pool uniformity without bias provides more unique targets per screen. This means less effort is required to achieve the data coverage you need, saving time and money.
Be data confident
With the precision and uniformity of Twist Oligo Pools, you can be confident in the quality of your experimental results. Don’t miss a datapoint due to synthesis errors or inefficiencies. Get industry-leading oligo representation and low error rate for oligos of any length up to 300nt, and pools of any size. Leverage these exceptional quality oligo pools at the precise scale and length required for your application.
Tell us what you would like more information on. We’re here to help!
Contact Us
Oligo Pools
Twist Oligo Pools are highly diverse collections of single-stranded oligonucleotides synthesized using our silicon-based DNA writing technology. Our synthesis platform enables massively parallel production of hundreds of thousands of high-quality, accurate oligos per run, allowing the generation of complex and diverse oligo pools for use in applications like CRISPR screening.
How does it work?
You provide us with your oligo sequences and we will synthesize your user designed oligo pool libraries, enabling you to spend your time working on experimentation and discovery.
Highly Uniform Oligo Synthesis
NGS quality control data from a typical oligo pool containing 23,000 90mer oligos show the uniformity of the pool at 300x read coverage. The corresponding table indicates the uniformity metrics for this pool. Twist oligos are synthesized bias-free with high uniformity and complete oligo representation.
Sequencing Analysis of Oligo Pools
Sequencing analysis of Oligo Pools generated by Twist Bioscience and an array-based competitor demonstrate that the Twist Oligo Pools contain 100% of the expected sequences and a higher percentage of correct sequences than the competitor pool.
Specifications
| Oligo Length | Up to 300nt |
| Oligo Pool Size | No limits on pool size |
| Yield | >0.2 fmol of each oligo |
| Uniformity | >90% oligos represented within <2.0x of the mean |
| Error rate | Up to 1:3000 |
| Turn-Around Time | Starting from 5 business days |
| Price | Industry-leading pricing |
Tell us what you would like more information on. We’re here to help!
Contact Us
Oligo Pools
Twist Oligo Pools are highly diverse collections of single-stranded oligonucleotides synthesized using our silicon-based DNA writing technology. Our synthesis platform enables massively parallel production of hundreds of thousands of high-quality, accurate oligos per run, allowing the generation of complex and diverse oligo pools for use in applications like CRISPR screening.
How does it work?
You provide us with your oligo sequences and we will synthesize your user designed oligo pool libraries, enabling you to spend your time working on experimentation and discovery.
Highly Uniform Oligo Synthesis
NGS quality control data from a typical oligo pool containing 23,000 90mer oligos show the uniformity of the pool at 300x read coverage. The corresponding table indicates the uniformity metrics for this pool. Twist oligos are synthesized bias-free with high uniformity and complete oligo representation.
Sequencing Analysis of Oligo Pools
Sequencing analysis of Oligo Pools generated by Twist Bioscience and an array-based competitor demonstrate that the Twist Oligo Pools contain 100% of the expected sequences and a higher percentage of correct sequences than the competitor pool.
Specifications
| Oligo Length | Up to 300nt |
| Oligo Pool Size | No limits on pool size |
| Yield | >0.2 fmol of each oligo |
| Uniformity | >90% oligos represented within <2.0x of the mean |
| Error rate | Up to 1:3000 |
| Turn-Around Time | Starting from 5 business days |
| Price | Industry-leading pricing |
Tell us what you would like more information on. We’re here to help!
Contact Us
New Cloned Oligo Pools
Oligo pool quality is the foundation of a successful experiment. Errors during synthesis or cloning can easily skew oligo pool quality, leading to over and underrepresentation of desired sequences. Twist now offers an optimized cloning process that allows you to further avoid the hardship that comes with testing PCR amplification conditions, selecting the right polymerases and primer pairs, as well as designing a cloning workflown workflow. Skip the drama and let Twist Bioscience synthesize and clone oligo pools for you.
How does it work?
You provide us with your oligo sequences and we will synthesize and clone your user designed oligo pool libraries, enabling you to spend your time working on experimentation and discovery.
Uniformity of linear and cloned oligo pools
An oligo pool comprised of 15,154 oligos was synthesized and amplified to evaluate the uniformity of the pool, as well as the percent of on-target oligos, and the percent of dropouts. The amplified pool was then cloned into a custom vector using the Twist in-house cloning strategies. A comparison of the NGS data of the pool pre and post cloning shows that the percent of on-target oligos remains >96%, with no dropouts and a difference of 0.1 between the two 90th/10th percentile ratios.
High quality cloned oligo pools
Figure 1 A and Figure 1 B summarize data collected from linear and cloned oligo pools comprised of 15154 oligos. These plots show the GC content of each oligo (x-axis) and the normalized count for that particular oligo (y-axis). This illustrates that there is limited bias in oligos based on GC content, and the oligo pool is uniformly distributed in counts, with limited dropouts. The 95th/5th percentile indicates that 90% of the oligos are within x multiplier of the mean. For linear oligo pools (figure 1A), 90% are within 2x of the mean. Following cloning, (figure 1B), 90% remain within 2x of the mean, showing that oligo pool quality and uniformity is maintained throughout Twist’s cloning process.
Specifications
| Oligo Length | Up to 100 nt |
| Yield | Starting at 50 ug |
| Uniformity | Uniform synthesis ensures excellent oligo representation with >95% of target oligos represented |
| Turn-Around Time | 6-8 weeks for new vectors, and 4-6 weeks for onboarded vectors |
Tell us what you would like more information on. We’re here to help!
Contact Us
New Cloned Oligo Pools
Oligo pool quality is the foundation of a successful experiment. Errors during synthesis or cloning can easily skew oligo pool quality, leading to over and underrepresentation of desired sequences. Twist now offers an optimized cloning process that allows you to further avoid the hardship that comes with testing PCR amplification conditions, selecting the right polymerases and primer pairs, as well as designing a cloning workflown workflow. Skip the drama and let Twist Bioscience synthesize and clone oligo pools for you.
How does it work?
You provide us with your oligo sequences and we will synthesize and clone your user designed oligo pool libraries, enabling you to spend your time working on experimentation and discovery.
Uniformity of linear and cloned oligo pools
An oligo pool comprised of 15,154 oligos was synthesized and amplified to evaluate the uniformity of the pool, as well as the percent of on-target oligos, and the percent of dropouts. The amplified pool was then cloned into a custom vector using the Twist in-house cloning strategies. A comparison of the NGS data of the pool pre and post cloning shows that the percent of on-target oligos remains >96%, with no dropouts and a difference of 0.1 between the two 90th/10th percentile ratios.
High quality cloned oligo pools
Figure 1 A and Figure 1 B summarize data collected from linear and cloned oligo pools comprised of 15154 oligos. These plots show the GC content of each oligo (x-axis) and the normalized count for that particular oligo (y-axis). This illustrates that there is limited bias in oligos based on GC content, and the oligo pool is uniformly distributed in counts, with limited dropouts. The 95th/5th percentile indicates that 90% of the oligos are within x multiplier of the mean. For linear oligo pools (figure 1A), 90% are within 2x of the mean. Following cloning, (figure 1B), 90% remain within 2x of the mean, showing that oligo pool quality and uniformity is maintained throughout Twist’s cloning process.
Specifications
| Oligo Length | Up to 100 nt |
| Yield | Starting at 50 ug |
| Uniformity | Uniform synthesis ensures excellent oligo representation with >95% of target oligos represented |
| Turn-Around Time | 6-8 weeks for new vectors, and 4-6 weeks for onboarded vectors |
Tell us what you would like more information on. We’re here to help!
Contact Us
Guide RNA libraries for CRISPR screens
CRISPR screens involve the precise, high-throughput knock-out, interference or modulation of gene activity, allowing complex genotype-phenotype interactions to be unveiled. Oligo Pools are designed to encode multiple guide RNA molecules per gene.
Product benefits include:
- Full control over guide library design, avoiding unwanted guides, wasted sequencing, and limited guide-per-gene ratios.
- Pairing high-fidelity Cas enzymes with highly accurate synthesis for true high-fidelity screens with minimal off-target effects.
- Incredibly uniform, bias-free pools achieve amplified signal to noise ratio to achieve maximum hit detection, lowering downstream costs.
- Oligos up to 300nt make space for additional experimental elements. Add multiple guides per oligo, genome homology, or minimal promoters with ease.
Watch our webinar: Next generation CRISPR screens based on single-cell transcriptomics
Genome tiling libraries
Tiling libraries allow a comprehensive study of genomic functionality. Pools of oligos may encode peptide arrays, mRNA encoding sequences, or genome fragments.
Product benefits include:
- Tiling precise genomic regions of interest. Reduce experimental noise by ignoring unwanted sequences at the design step.
- Complex, repetitive or palindromic sequences that cannot typically be synthesized as genes can be encoded in oligos.
- Larger peptide structures or genome architectures can be encoded in oligos 300nt in length.
- Uniform synthesis minimizes oversampling required for comprehensive screening.
Read our article: Identifying Coronavirus Virulence Factors for RNA Vaccine Development with Twist Oligo Pools
High throughput genetic control screens
Understanding the genetic elements controlling gene expression and regulation has broad application in synthetic biology and development of potential disease treatment. Oligo Pools power high-throughput screens of genetic control, including gene expression profiling and reporter-based applications.
Product benefits include:
- High-quality pools ensure you can interrogate every base for functionality
- High uniformity means you screen less to find your answers
- Up to 300nt pools gives you the flexibility to add multiple elements or homology regions
Watch our webinar: Epitranscriptome Screening on TechNetworks
Fluorescence in-situ hybridization
Fluorescence in-situ hybridization (FISH) allows the abundance and spatial organization of nucleic acids in biological samples to be directly visualized. Oligo Pools can encode probes that when bound to fluorescent markers provide tunable, programmable visualization of large numbers of targets.
Product benefits include:
- Rapid FISH probe development
- Sequence control enables effective targeting
- Longer oligos enable more specific targeting
Watch our webinar: Rapid and efficient production of FISH probes using oligo pools with Nicola Crosetto
Guide RNA libraries for CRISPR screens
CRISPR screens involve the precise, high-throughput knock-out, interference or modulation of gene activity, allowing complex genotype-phenotype interactions to be unveiled. Oligo Pools are designed to encode multiple guide RNA molecules per gene.
Product benefits include:
- Full control over guide library design, avoiding unwanted guides, wasted sequencing, and limited guide-per-gene ratios.
- Pairing high-fidelity Cas enzymes with highly accurate synthesis for true high-fidelity screens with minimal off-target effects.
- Incredibly uniform, bias-free pools achieve amplified signal to noise ratio to achieve maximum hit detection, lowering downstream costs.
- Oligos up to 300nt make space for additional experimental elements. Add multiple guides per oligo, genome homology, or minimal promoters with ease.
Watch our webinar: Next generation CRISPR screens based on single-cell transcriptomics
Genome tiling libraries
Tiling libraries allow a comprehensive study of genomic functionality. Pools of oligos may encode peptide arrays, mRNA encoding sequences, or genome fragments.
Product benefits include:
- Tiling precise genomic regions of interest. Reduce experimental noise by ignoring unwanted sequences at the design step.
- Complex, repetitive or palindromic sequences that cannot typically be synthesized as genes can be encoded in oligos.
- Larger peptide structures or genome architectures can be encoded in oligos 300nt in length.
- Uniform synthesis minimizes oversampling required for comprehensive screening.
Read our article: Identifying Coronavirus Virulence Factors for RNA Vaccine Development with Twist Oligo Pools
High throughput genetic control screens
Understanding the genetic elements controlling gene expression and regulation has broad application in synthetic biology and development of potential disease treatment. Oligo Pools power high-throughput screens of genetic control, including gene expression profiling and reporter-based applications.
Product benefits include:
- High-quality pools ensure you can interrogate every base for functionality
- High uniformity means you screen less to find your answers
- Up to 300nt pools gives you the flexibility to add multiple elements or homology regions
Watch our webinar: Epitranscriptome Screening on TechNetworks
Fluorescence in-situ hybridization
Fluorescence in-situ hybridization (FISH) allows the abundance and spatial organization of nucleic acids in biological samples to be directly visualized. Oligo Pools can encode probes that when bound to fluorescent markers provide tunable, programmable visualization of large numbers of targets.
Product benefits include:
- Rapid FISH probe development
- Sequence control enables effective targeting
- Longer oligos enable more specific targeting
Watch our webinar: Rapid and efficient production of FISH probes using oligo pools with Nicola Crosetto
Webinar
Discovering PARP inhibitor resistance mechanisms using genome-wide and focused CRISPR screens
Protocol
These guidelines outline the best practices for resuspending Twist Gene Fragments, Clonal Genes, Oligo Pools, and Variant Libraries.
Webinar
Discovering PARP inhibitor resistance mechanisms using genome-wide and focused CRISPR screens
Protocol
These guidelines outline the best practices for resuspending Twist Gene Fragments, Clonal Genes, Oligo Pools, and Variant Libraries.
