Start Customizing Your Genes
Start Customizing Your Genes
Start Customizing Your Genes
OVERVIEW CLONAL GENES GENE FRAGMENTS RESOURCES REFERENCES
OVERVIEW

New Features For Clonal Genes

We’ve enhanced our Clonal Genes products with additional plasmid prep sizes and other options including normalization, endotoxin-free DNA, and a variety of tube and plate options to meet your research needs.

twist new clonal genes feature

Performance At Scale

Our silicon-based, high-precision DNA synthesis platform results in higher quality genes and significantly increased throughput and scalability.


We offer both Clonal Genes and Gene Fragments for your research needs:
Clonal Genes are synthesized DNA cloned into a plasmid vector and NGS sequence verified.
Gene Fragments are linear synthesized DNA sequences for direct cloning or larger gene assembly.


Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery.
 

 

TWIST GENES IN ACTION

Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated
antibody development process that aims to provide access
to treatments within 60 days.

Read the Case Study

pandemic prevention platform

New Features For Clonal Genes

We’ve enhanced our Clonal Genes products with additional plasmid prep sizes and other options including normalization, endotoxin-free DNA, and a variety of tube and plate options to meet your research needs.

twist new clonal genes feature

Performance At Scale

Our silicon-based, high-precision DNA synthesis platform results in higher quality genes and significantly increased throughput and scalability.


We offer both Clonal Genes and Gene Fragments for your research needs:
Clonal Genes are synthesized DNA cloned into a plasmid vector and NGS sequence verified.
Gene Fragments are linear synthesized DNA sequences for direct cloning or larger gene assembly.


Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery.
 

 

TWIST GENES IN ACTION

Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated
antibody development process that aims to provide access
to treatments within 60 days.

Read the Case Study

pandemic prevention platform
Clonal Genes

Clonal Genes

Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.

Order now or Get the Product Sheet.

 Low Cost – High Quality clonal gene
Low Cost – High Quality
From 9¢ per bp
No hidden sub-cloning or DNA complexity fees
From 11 business days
DNA Your Way clonal gene
DNA Your Way
0.3 – 5 kb genes cloned into a plasmid of your choice
Choose a Twist Catalog Vector or send us yours
4 prep scales (50ng – 2µg | 2µg – 10µg | 10µg – 100µg | 100µg – 1mg)
Normalization and endotoxin free options available
Scalable Synthesis clonal gene
Scalable Synthesis
No order limits
Same turnaround regardless of order size
Sequence Perfect Genes

A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.

 

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Single Bar Graph
Clonal Genes Success Rate

Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.

 

Order now

Sequencing Graph

Clonal Genes

Twist Bioscience’s platform is capable of synthesizing thousands of genes each day to meet all your DNA needs. Our silicon-based scalability and high precision oligo production results in high quality DNA synthesis and assembly; qualities that allow Twist to create perfect genes of various lengths and difficulty, NGS-verified and delivered on-time when you need them.

Order now or Get the Product Sheet.

 Low Cost – High Quality clonal gene
Low Cost – High Quality
From 9¢ per bp
No hidden sub-cloning or DNA complexity fees
From 11 business days
DNA Your Way clonal gene
DNA Your Way
0.3 – 5 kb genes cloned into a plasmid of your choice
Choose a Twist Catalog Vector or send us yours
4 prep scales (50ng – 2µg | 2µg – 10µg | 10µg – 100µg | 100µg – 1mg)
Normalization and endotoxin free options available
Scalable Synthesis clonal gene
Scalable Synthesis
No order limits
Same turnaround regardless of order size
Sequence Perfect Genes

A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.

 

Order now

Single Bar Graph
Clonal Genes Success Rate

Twist Bioscience’s observed success rate for clonal genes. The success rate is defined as the percentage of clonal genes ordered that were delivered without any SNPs or indels.

 

Order now

Sequencing Graph
Gene Fragments

Gene Fragments

Twist Bioscience Gene Fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.

Order Now or Get the Product Sheet.

Fast and Economical
Fast and Economical
7¢ per bp
6 to 9 business days
Gene Fragments for assembly and cloning
Compatible with many downstream cloning methods
Screen Less Discover More Gene Fragments
Screen Less Discover More
Lowest error rate 1:3000
100 ng – 1 µg yield
300 bp – 1800 bp gene fragments
Order as little as one gene fragment
Reduce Costs With High Quality

Comparison of total costs for DNA synthesis, cloning, and sequence verification of constructs built with gene fragments. Cloning cost is assumed to be $30 per gene and sequencing cost is assumed to be $5 per clone sequenced. Sequencing more clones of fragments synthesized by the competitor is required to obtain correct sequence with 99% confidence, increasing the total cost for each gene.

 

 ORDER NOW     

list price of gene fragment graph
Performance for Less

Twist’s DNA synthesis technology exhibits extremely low error rates, the average error rate is one in 3,000 base pairs. This level of precision makes larger gene assembly more reliable and easier to achieve.


 ORDER NOW

oilgouncleotide synthesis error rate graph
Screen Less

Accurate fragment synthesis accelerates discovery. Twist Bioscience gene fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones.

 

ORDER NOW

percentage of correct constructs

Gene Fragments

Twist Bioscience Gene Fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.

Order Now or Get the Product Sheet.

Fast and Economical
Fast and Economical
7¢ per bp
6 to 9 business days
Gene Fragments for assembly and cloning
Compatible with many downstream cloning methods
Screen Less Discover More Gene Fragments
Screen Less Discover More
Lowest error rate 1:3000
100 ng – 1 µg yield
300 bp – 1800 bp gene fragments
Order as little as one gene fragment
Reduce Costs With High Quality

Comparison of total costs for DNA synthesis, cloning, and sequence verification of constructs built with gene fragments. Cloning cost is assumed to be $30 per gene and sequencing cost is assumed to be $5 per clone sequenced. Sequencing more clones of fragments synthesized by the competitor is required to obtain correct sequence with 99% confidence, increasing the total cost for each gene.

 

 ORDER NOW     

list price of gene fragment graph
Performance for Less

Twist’s DNA synthesis technology exhibits extremely low error rates, the average error rate is one in 3,000 base pairs. This level of precision makes larger gene assembly more reliable and easier to achieve.


 ORDER NOW

oilgouncleotide synthesis error rate graph
Screen Less

Accurate fragment synthesis accelerates discovery. Twist Bioscience gene fragments enable you to build more constructs and minimize the time and cost of screening for perfect clones.

 

ORDER NOW

percentage of correct constructs
RESOURCES
REFERENCES
Testimonials
The combination of price and service allows us to do science that we could not otherwise do
PROFESSOR NICHOLAS HARMER
University of Exeter
The quality has been excellent, and I find that cloning with these genes is successful more often on the first time than with DNA fragments from other companies
DR. AMANDA PORTILLO
Institute of Systems and Synthetic Biology
This is where we came to really like the online ordering portal, that tells you then and there whether Twist can make your gene. We input our sequences and Twist’s algorithms right away said “yes, why not?”. It turned out that for Twist, making these difficult sequences was easy.
DR. PHILIPPE MARLIERE
Institute of Systems and Synthetic Biology

Genes Calculator

Gene Fragments*
Clonal Genes*
AVERAGE LENGTH

0.3kb

0.6kb

0.9kb

1.2kb

1.5kb

1.8kb
base pairs
Please enter a quantity between 300 and 1800.
QUANTITY
genes
Please enter a quantity between 1 and 10000.
ESTIMATED TOTAL
Based on cloning in a Twist vector
$54,000
TWIST CLONAL GENES WILL SAVE YOU AN ESTIMATED**
TWIST SAVES YOU AN ESTIMATED**
$1200on reagents & kits
BREAKDOWN
Vector prep
$45
Amplification
$35
Cloning
$60
QC
$40
TOTAL
~ $180 per gene
120 hrsof manual cloning
Manual cloning takes about 6 hours per 96 genes
ORDER NOW
SAVINGS WITH TWIST
COMPARED TO***
30%
SAVINGS
*Twist products are subject to certain use restrictions as set forth in Twist's Supply Terms and Conditions.
**Cost savings based on comparison to public list prices for standard reagents. Time savings based on Twist's internal testing of typical times for standard manual cloning.
***Savings based on comparison to competitor’s publicly-listed pricing as of May 15, 2019. Turn-around times between Twist and competitors may vary.

Finally, an easy way to order DNA

 

Instantly know if your sequence is a go
Our smart algorithm lets you know in seconds if your sequence can be synthesized or not

Your quote is now just one click away
No more wasted time waiting for surprise quotes. See real-time pricing when you submit your data

Stay in the know from checkout to your door
We update you at every stage of your orders’ progress, giving you comprehensive insight on crucial production details

Intuitive usability meets bulletproof security
Upload your sequences with the comfort of knowing your proprietary data is protected

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