The Power of Silicon-based DNA Synthesis
Twist Bioscience’s high precision, silicon-based high-throughput gene synthesis platform enables us to produce high quality gene fragments starting at 7¢ (USD) per bp and NGS-verified sequence perfect clonal genes starting at 9¢ (USD) per bp. What does this mean for you? Rapid and accurate DNA synthesis at the scale you need for the price you want.
What do we offer?
Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery. Still not convinced? Talk to one of our resident experts!
Gene Fragments
Linear synthesized DNA sequences for direct cloning or larger gene assembly.
Express Genes and Clonal Genes
Synthesized DNA cloned into a plasmid vector and NGS sequence verified.
How It Works
Normally, genes are synthesized with one gene per 96 well plate. Here at Twist, we have perfected gene synthesis and with our proprietary platform is able to synthesize 9600 genes per one silicon-based chip in a single run. This means we can produce 1 gene or 100,000 genes in parallel, in the same amount of time.
Get your genes faster than ever without compromising sequence integrity or quality with Twist Bioscience. Here’s what to do next:
All you have to do is design your custom sequence and our high quality gene synthesis platform will do the rest.
For Research Use Only. Not for Diagnostic Procedures.
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.
Testimonials
Featured Resources
Application Note
Webinar
Ordering online just got easier. Watch our “How to” video to see how to order genes all online from our eCommerce platform.
The Power of Silicon-based DNA Synthesis
Twist Bioscience’s high precision, silicon-based high-throughput gene synthesis platform enables us to produce high quality gene fragments starting at 7¢ (USD) per bp and NGS-verified sequence perfect clonal genes starting at 9¢ (USD) per bp. What does this mean for you? Rapid and accurate DNA synthesis at the scale you need for the price you want.
What do we offer?
Twist's Genes offering gives you the flexibility to get the DNA you want, the way you want it. Think bigger, expand your design scope, and accelerate discovery. Still not convinced? Talk to one of our resident experts!
Gene Fragments
Linear synthesized DNA sequences for direct cloning or larger gene assembly.
Express Genes and Clonal Genes
Synthesized DNA cloned into a plasmid vector and NGS sequence verified.
How It Works
Normally, genes are synthesized with one gene per 96 well plate. Here at Twist, we have perfected gene synthesis and with our proprietary platform is able to synthesize 9600 genes per one silicon-based chip in a single run. This means we can produce 1 gene or 100,000 genes in parallel, in the same amount of time.
Get your genes faster than ever without compromising sequence integrity or quality with Twist Bioscience. Here’s what to do next:
All you have to do is design your custom sequence and our high quality gene synthesis platform will do the rest.
For Research Use Only. Not for Diagnostic Procedures.
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Testimonials
Featured Resources
Ordering online just got easier. Watch our “How to” video to see how to order genes all online from our eCommerce platform.
Clonal Genes
Twist Bioscience’s platform is capable of synthesizing hundreds of thousands of genes each month to meet all your DNA needs. Our silicon-based platform for DNA synthesis enables us to create at scale, highly precise, sequence perfect clonal genes of various lengths and difficulty that are all NGS verified and now produced faster than ever before. We are excited to announce our new Express Genes service, which provides you with NGS-verified Clonal Genes, shipped in as few as 5 days.*
Introducing our new Express Genes service
Twist Bioscience’s Express Genes service provides you with the same NGS-verified, sequence perfect Clonal Genes you would expect from our standard Clonal Genes but with the added bonus of an all time fast order to ship turnaround time of 5-7 business days.
See below for our full Clonal Genes offering:
Sequence perfect Clonal Genes
A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.
*Terms and Conditions: Eligible Express Genes ship in 5-7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Average turnaround time for Clonal Genes is 10-15 business days. New vector onboarding for both Express Genes and Clonal Genes will add additional time. Pricing calculator only refers to Gene Fragments and Clonal Genes offering, not inclusive of Express Genes service pricing.
For Research Use Only. Not for Diagnostic Procedures.
FAQ
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Clonal Genes
Twist Bioscience’s platform is capable of synthesizing hundreds of thousands of genes each month to meet all your DNA needs. Our silicon-based platform for DNA synthesis enables us to create at scale, highly precise, sequence perfect clonal genes of various lengths and difficulty that are all NGS verified and now produced faster than ever before. We are excited to announce our new Express Genes service, which provides you with NGS-verified Clonal Genes, shipped in as few as 5 days.*
Introducing our new Express Genes service
Twist Bioscience’s Express Genes service provides you with the same NGS-verified, sequence perfect Clonal Genes you would expect from our standard Clonal Genes but with the added bonus of an all time fast order to ship turnaround time of 5-7 business days.
See below for our full Clonal Genes offering:
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Sequence perfect Clonal Genes
A graphical representation of our standard NGS-verification performed on every clonal gene. The clonal gene in this figure is an example of an error-free clone. The read depth is indicated for the entire plasmid and no SNPs or indels were detected.
*Terms and Conditions: Eligible Express Genes ship in 5-7 business days. This time will vary based on complexity and length of the sequence. Orders placed outside of the US will incur additional delivery turnaround time. Turnaround time for Clonal Genes is subject to change based on customizations and complexity. Average turnaround time for Clonal Genes is 10-15 business days. New vector onboarding for both Express Genes and Clonal Genes will add additional time. Pricing calculator only refers to Gene Fragments and Clonal Genes offering, not inclusive of Express Genes service pricing.
For Research Use Only. Not for Diagnostic Procedures.
FAQ
Gene Fragments
Synthetic Gene Fragments are an inexpensive, rapid, and efficient way to build the genes you need for your research. Gene Fragments from Twist Bioscience improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs.
Think bigger, design on a grander scale, and accelerate your discoveries.
Order Now or Get the Product Sheet.
Twist’s DNA synthesis technology outperforms the competitions with exceptionally low error rates.
In a direct comparison of Gene Fragment products, Twist consistently showed the lowest error rate. The graph to the left represents the average error rate for Gene sequences ordered from Twist and Integrated DNA Technologies, Inc. (IDT). The results show Twist Gene Fragments have greater sequence accuracy over eBlocks and gBlocks with an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over gBlocks.
More perfect Genes from Twist
Twist Gene Fragments consistently yield the highest percentage of perfect clones which saves you time and money in your research. The graph to the left shows a direct comparison of the percentage of sequence perfect clones across a wide variety of gene lengths and sequences for 3 different gene products. Data was generated using a set of 63 sequences with a wide variety of gene lengths to reflect the diversity of gene lengths required for various real-world synthetic biology applications.
FAQ
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.
Gene Fragments
Synthetic Gene Fragments are an inexpensive, rapid, and efficient way to build the genes you need for your research. Gene Fragments from Twist Bioscience improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs.
Think bigger, design on a grander scale, and accelerate your discoveries.
Order Now or Get the Product Sheet.
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Twist’s DNA synthesis technology outperforms the competitions with exceptionally low error rates.
In a direct comparison of Gene Fragment products, Twist consistently showed the lowest error rate. The graph to the left represents the average error rate for Gene sequences ordered from Twist and Integrated DNA Technologies, Inc. (IDT). The results show Twist Gene Fragments have greater sequence accuracy over eBlocks and gBlocks with an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over gBlocks.
More perfect Genes from Twist
Twist Gene Fragments consistently yield the highest percentage of perfect clones which saves you time and money in your research. The graph to the left shows a direct comparison of the percentage of sequence perfect clones across a wide variety of gene lengths and sequences for 3 different gene products. Data was generated using a set of 63 sequences with a wide variety of gene lengths to reflect the diversity of gene lengths required for various real-world synthetic biology applications.
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.
FAQ
Product Sheet
Clonal Genes Product Sheet
Twist Bioscience is transforming gene synthesis, a process at the core of synthetic and molecular biology. Our silicon-based DNA writing platform significantly increases gene synthesis throughput and scalability, while also reducing turnaround time and price per base.
Product Sheet
Gene Fragments Product Sheet
Twist Bioscience gene fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs。
Case Study
Build a better DNA Assembly Line
Arzeda leverages technology from Labcyte, TeselaGen and Twist Bioscience to accelerate their synthetic biology workflow.
Application Note
Pathway Engineering Through 5 kb Gene Synthesis
A rapid design-build-test cycle is vital to metabolic engineering applications in which researchers re-program microorganisms to produce non-native molecules of interest. To produce these molecules, researchers typically add one gene or an entire pathway of genes into a host organism. These genes encode proteins or enzymes that, when expressed in a microbial host, convert a common substrate into a molecule of interest.
Case Study
Speeding Up Antibody Discovery for Infectious Disease
Tasked with an ambitious goal from DARPA to develop a rapid response to help medical workers fight viral diseases in the field, Vanderbilt University Medical Center has already reduced the time to develop antibodies significantly. High-throughput, synthetic genes from Twist Bioscience have allowed the lab to expedite this process.
Webinar
Amyris discusses Next-Generation Platforms for Strain Optimization
Watch this webinar showing how Twist Genes are enabling this innovative company with their microbial engineering efforts.https://www.twistbioscience.com/genes_bioengineeringwebinar_sunilchandran
Product Sheet
Twist Vectors Product Sheet
Twist Bioscience synthesizes high-quality, NGS-verified custom genes at a… cost and scale that are otherwise unavailable. For researchers wanting to replicate their synthetic genes or use them in expression studies, Twist provides the option of delivery in a diverse selection of cloning and expression vectors through a convenient online ordering platform.
Application Note
Twist Tips for Genes: How to Design Your Gene
Twist Bioscience uses a silicon-based DNA synthesis platform to generate… high-quality synthetic DNA. Designing and ordering genes from Twist Bioscience is simple when using our online ordering platform. This document provides tips for optimizing and customizing Twist Clonal Genes and Gene Fragments for a variety of applications
Application Note
Twist Tips for Genes - Meeting Minimum Length Requirements for Genes in Custom Vectors
When you submit a sequence for synthesis, the scoring algorithm checks the sequence to determine whether it can be synthesized, and one of the first things it checks is whether the sequence meets the minimum length requirement of 300 bp. Use this document to help you design your Genes when they do not meet the minimum length requirement.
Application Note
From Design to Protein: Cell-Free Protein Expression Using Twist Gene Fragments
Application Note
Gene Fragments Cloning Guidelines
Twist Bioscience’s Gene Fragments are double-stranded DNA, 300 to… 1800 base pairs in length. These high-quality fragments come with unique 5’ and 3’ adapter sequences.
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Product Sheet
Clonal Genes Product Sheet
Twist Bioscience is transforming gene synthesis, a process at the core of synthetic and molecular biology. Our silicon-based DNA writing platform significantly increases gene synthesis throughput and scalability, while also reducing turnaround time and price per base.
Product Sheet
Gene Fragments Product Sheet
Twist Bioscience gene fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs。
Case Study
Build a better DNA Assembly Line
Arzeda leverages technology from Labcyte, TeselaGen and Twist Bioscience to accelerate their synthetic biology workflow.
Application Note
Pathway Engineering Through 5 kb Gene Synthesis
A rapid design-build-test cycle is vital to metabolic engineering applications in which researchers re-program microorganisms to produce non-native molecules of interest. To produce these molecules, researchers typically add one gene or an entire pathway of genes into a host organism. These genes encode proteins or enzymes that, when expressed in a microbial host, convert a common substrate into a molecule of interest.
Case Study
Speeding Up Antibody Discovery for Infectious Disease
Tasked with an ambitious goal from DARPA to develop a rapid response to help medical workers fight viral diseases in the field, Vanderbilt University Medical Center has already reduced the time to develop antibodies significantly. High-throughput, synthetic genes from Twist Bioscience have allowed the lab to expedite this process.
Webinar
Amyris discusses Next-Generation Platforms for Strain Optimization
Watch this webinar showing how Twist Genes are enabling this innovative company with their microbial engineering efforts.https://www.twistbioscience.com/genes_bioengineeringwebinar_sunilchandran
Product Sheet
Twist Vectors Product Sheet
Twist Bioscience synthesizes high-quality, NGS-verified custom genes at a… cost and scale that are otherwise unavailable. For researchers wanting to replicate their synthetic genes or use them in expression studies, Twist provides the option of delivery in a diverse selection of cloning and expression vectors through a convenient online ordering platform.
Application Note
Twist Tips for Genes: How to Design Your Gene
Twist Bioscience uses a silicon-based DNA synthesis platform to generate… high-quality synthetic DNA. Designing and ordering genes from Twist Bioscience is simple when using our online ordering platform. This document provides tips for optimizing and customizing Twist Clonal Genes and Gene Fragments for a variety of applications
Application Note
Twist Tips for Genes - Meeting Minimum Length Requirements for Genes in Custom Vectors
When you submit a sequence for synthesis, the scoring algorithm checks the sequence to determine whether it can be synthesized, and one of the first things it checks is whether the sequence meets the minimum length requirement of 300 bp. Use this document to help you design your Genes when they do not meet the minimum length requirement.
Application Note
From Design to Protein: Cell-Free Protein Expression Using Twist Gene Fragments
Application Note
Gene Fragments Cloning Guidelines
Twist Bioscience’s Gene Fragments are double-stranded DNA, 300 to… 1800 base pairs in length. These high-quality fragments come with unique 5’ and 3’ adapter sequences.
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Article
Machine Learning-Guided Prediction of Antigen-Reactive In Silico Clonotypes Based on Changes in Clonal Abundance through Bio-Panning
Article
The LRRC8A:C Heteromeric Channel Is a cGAMP Transporter and the Dominant cGAMP Importer in Human Vasculature Cells
Article
De novo protein design enables precise induction of functional antibodies in vivo
Article
In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Article
Machine Learning-Guided Prediction of Antigen-Reactive In Silico Clonotypes Based on Changes in Clonal Abundance through Bio-Panning
Article
The LRRC8A:C Heteromeric Channel Is a cGAMP Transporter and the Dominant cGAMP Importer in Human Vasculature Cells
Article
De novo protein design enables precise induction of functional antibodies in vivo
Article
In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
General
Clonal Genes
Codon Optimization
Sequence Design
**Savings based on comparison to competitor’s publicly listed pricing as of August 15, 2022. Turnaround times between Twist and competitors may vary.
T&Cs: Turnaround time for the genes products are subject to change based on customizations and complexity. Average turnaround time for Gene Fragments with adapters is 3-6 business days and Gene Fragments without adapters is 4-8 business days. New vector onboarding for both Gene Fragments and Clonal Genes will add additional time
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
TOTAL
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Twist Expression Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Custom Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
