Built for Whole Genomes: Comprehensive Coverage without Bias
The Twist PCR Free WGS Library Preparation Kit is engineered to generate high quality whole genome libraries without amplification, preserving native genome representation. Built on Twist’s optimized enzymatic fragmentation and ligation chemistry, the workflow delivers consistent insert sizes, low ligation bias, and uniform coverage across the genome, supporting accurate variant characterization.
Lower AT dropout across the genome
PCR free library preparation preserves native genome
representation, reducing AT-related dropout and improving uniform coverage across challenging
regions.
Built for scaling up workflows
Optimized performance with Twist's full length UDI adapters support multiplexing up to 1,536 samples in a single run.
Maximize Conversion. Minimize Bias.
Minimized ligation bias and improved conversion efficiency preserve true genome representation and generate more usable sequencing reads from every sample.
Built for Whole Genomes: Comprehensive Coverage without Bias
The Twist PCR Free WGS Library Preparation Kit is engineered to generate high quality whole genome libraries without amplification, preserving native genome representation. Built on Twist’s optimized enzymatic fragmentation and ligation chemistry, the workflow delivers consistent insert sizes, low ligation bias, and uniform coverage across the genome, supporting accurate variant characterization.
Lower AT dropout across the genome
PCR free library preparation preserves native genome
representation, reducing AT-related dropout and improving uniform coverage across challenging
regions.
Built for scaling up workflows
Optimized performance with Twist's full length UDI adapters support multiplexing up to 1,536 samples in a single run.
Maximize Conversion. Minimize Bias.
Minimized ligation bias and improved conversion efficiency preserve true genome representation and generate more usable sequencing reads from every sample.
Product data
Figure 1. PCR-free library yield comparison across inputs. Final library concentration (nM) was measured following PCR-free library preparation using the indicated DNA inputs. Twist PCR-Free WGS Library Preparation generated higher library yields than competitor workflows at equivalent inputs and maintained strong performance at lower inputs.
Figure 2. The kit produces consistently large inserts across a wide range of DNA samples. (A) Median Insert Size is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 DNA sample input. (B) Percent Overlap (which measures how much paired-end sequencing reads redundantly cover the same DNA bases) is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 sample input.
Figure 3. Tunability of Twist PCR-Free WGS Library Prep Kit.
A: Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C.
Figure 4. The Twist PCR-Free WGS Library Preparation Kit provides more consistent coverage distribution across varying GC content. The kit shows minimized GC bias even at low inputs, thereby reducing the need for additional sequencing to achieve uniform coverage and better detection of variants in the high- and low-GC content regions.
Product data
Engineered to Preserve Library Yield at Low Input
Twist PCR-Free WGS Library Prep delivers robust library yields and consistent library size even as DNA input is reduced. Engineered ligase chemistry drives efficient, uniform ligation, helping maintain sequencing ready libraries across input levels without amplification.
Figure 1. High library yield and estimated library size. (A) Low DNA input has usable library yield. (B) Library diversity is maintained even with low DNA input. Twist PCR-Free WGS Library Preparation Kit libraries were prepared using NA12878 300 ng, 75 ng, and 37.5 ng genomic DNA.
Reliable Insert Size Control Across Inputs
Twist PCR-Free WGS Library Prep maintains consistent median insert sizes from 300 ng down to 37.5 ng DNA input. Minimal read overlap across samples ensures efficient sequencing and dependable genome wide coverage.
Figure 2. The kit produces consistently large inserts across a wide range of DNA samples. (A) Median Insert Size is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 DNA sample input. (B) Percent Overlap (which measures how much paired-end sequencing reads redundantly cover the same DNA bases) is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 sample input.
Controllable Fragment Lengths for More Efficient Sequencing
Twist TrueAmp Library Preparation Kits enable precise control of insert size across a broad range, allowing users to tune library preparation to the specific requirements of their sequencing workflows.
Figure 3. Tunability of Twist PCR-Free WGS Library Prep Kit.
A: Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C.
Minimized GC Bias for More Uniform Coverage
Across DNA inputs, Twist PCR-Free WGS Library Prep maintains consistent coverage across GC content.
Figure 4. The Twist PCR-Free WGS Library Preparation Kit provides more consistent coverage distribution across varying GC content. The kit shows minimized GC bias even at low inputs, thereby reducing the need for additional sequencing to achieve uniform coverage and better detection of variants in the high- and low-GC content regions.
Product data
Figure 1. PCR-free library yield comparison across inputs. Final library concentration (nM) was measured following PCR-free library preparation using the indicated DNA inputs. Twist PCR-Free WGS Library Preparation generated higher library yields than competitor workflows at equivalent inputs and maintained strong performance at lower inputs.
Figure 2. The kit produces consistently large inserts across a wide range of DNA samples. (A) Median Insert Size is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 DNA sample input. (B) Percent Overlap (which measures how much paired-end sequencing reads redundantly cover the same DNA bases) is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 sample input.
Figure 3. Tunability of Twist PCR-Free WGS Library Prep Kit.
A: Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C.
Figure 4. The Twist PCR-Free WGS Library Preparation Kit provides more consistent coverage distribution across varying GC content. The kit shows minimized GC bias even at low inputs, thereby reducing the need for additional sequencing to achieve uniform coverage and better detection of variants in the high- and low-GC content regions.
Product data
Engineered to Preserve Library Yield at Low Input
Twist PCR-Free WGS Library Prep delivers robust library yields and consistent library size even as DNA input is reduced. Engineered ligase chemistry drives efficient, uniform ligation, helping maintain sequencing ready libraries across input levels without amplification.
Figure 1. High library yield and estimated library size. (A) Low DNA input has usable library yield. (B) Library diversity is maintained even with low DNA input. Twist PCR-Free WGS Library Preparation Kit libraries were prepared using NA12878 300 ng, 75 ng, and 37.5 ng genomic DNA.
Reliable Insert Size Control Across Inputs
Twist PCR-Free WGS Library Prep maintains consistent median insert sizes from 300 ng down to 37.5 ng DNA input. Minimal read overlap across samples ensures efficient sequencing and dependable genome wide coverage.
Figure 2. The kit produces consistently large inserts across a wide range of DNA samples. (A) Median Insert Size is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 DNA sample input. (B) Percent Overlap (which measures how much paired-end sequencing reads redundantly cover the same DNA bases) is represented for 300 ng, 75 ng, and 37.5 ng of NA12878 sample input.
Controllable Fragment Lengths for More Efficient Sequencing
Twist TrueAmp Library Preparation Kits enable precise control of insert size across a broad range, allowing users to tune library preparation to the specific requirements of their sequencing workflows.
Figure 3. Tunability of Twist PCR-Free WGS Library Prep Kit.
A: Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C.
Minimized GC Bias for More Uniform Coverage
Across DNA inputs, Twist PCR-Free WGS Library Prep maintains consistent coverage across GC content.
Figure 4. The Twist PCR-Free WGS Library Preparation Kit provides more consistent coverage distribution across varying GC content. The kit shows minimized GC bias even at low inputs, thereby reducing the need for additional sequencing to achieve uniform coverage and better detection of variants in the high- and low-GC content regions.
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Product Sheet
Protocol
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Related Workflows and Applications
Twist offers a comprehensive suite of library preparation kits and accessory products, empowering you to tailor your WGS workflows to your unique needs.
Related Workflows and Applications
Twist offers a comprehensive suite of library preparation kits and accessory products, empowering you to tailor your WGS workflows to your unique needs.
