OVERVIEW DATA WORKFLOW ORDERING AND SPECS RESOURCES
Overview

For best-in-class results, use best-in-class tools


Twist Exome 2.0 is designed to detect rare and inherited diseases, as well as germline cancers. This panel’s high uniformity and low off-target rate deliver best-in-class sequencing efficiency, enabling quality data to be collected with less sequencing. With superior coverage of major genetic databases (RefSeq, CCDS, GenCode, Clinvar, ACMG73 and more) and the addition of clinically relevant non-coding pathogenic and likely pathogenic variants, Twist Exome 2.0 provides the value of multiple clinical panels all wrapped into one, easily customizable package.

Twist Exome 2.0 Coverage That Matters
Coverage That Matters
Covers 36.5 Mb of human protein coding regions
Based on recent database releases
Includes carefully curated clinical content
Twist Exome 2.0 Benefit Coverage That Matters
Best-in-Class Performance
Uniform enrichment of target regions
NGS QC of all probes
Best-in-Class Performance Retain Flexibility
Retain Flexibility
Easily spike-in content into the exome
Effective across multiplex target enrichment workflows
Run overnight or same day workflows
Compromise no more.

"We recently adapted the Twist Exome 2.0 for our work for various indications, especially for unexplained developmental disorders. The content, performance and quality are the best we have experienced from any provider, and we didn’t have to choose between robust content and performance." - Oliver Wachter of the Center for Human Genetics and Laboratory Diagnostics in Germany (MVZ-Martinsried GmbH)

Detecting the Unexpected

Did you know that most commercially available exome panels cover nearly all gene-coding sequences in the genome? But, one key and critical differentiating factor is how deeply they cover specific genes. While some whole-exome panels focus on providing particularly deep coverage over genes that are relevant to cancer development, inherited disorders, or other such conditions, the Twist Exome 2.0 provides deep coverage over all genes that have been linked to clinical phenotypes, enabling translational research on a wide range of conditions. 

Learn More

Human Exome 2.0 Plus Comprehensive Exome Spike-in

Our latest human exome product spiked-in with all the Twist Comprehensive Exome targets that are omitted in Exome 2.0. You can now order this product, check resources tab to find SKU information.

For best-in-class results, use best-in-class tools


Twist Exome 2.0 is designed to detect rare and inherited diseases, as well as germline cancers. This panel’s high uniformity and low off-target rate deliver best-in-class sequencing efficiency, enabling quality data to be collected with less sequencing. With superior coverage of major genetic databases (RefSeq, CCDS, GenCode, Clinvar, ACMG73 and more) and the addition of clinically relevant non-coding pathogenic and likely pathogenic variants, Twist Exome 2.0 provides the value of multiple clinical panels all wrapped into one, easily customizable package.

Twist Exome 2.0 Coverage That Matters
Coverage That Matters
Covers 36.5 Mb of human protein coding regions
Based on recent database releases
Includes carefully curated clinical content
Twist Exome 2.0 Benefit Coverage That Matters
Best-in-Class Performance
Uniform enrichment of target regions
NGS QC of all probes
Best-in-Class Performance Retain Flexibility
Retain Flexibility
Easily spike-in content into the exome
Effective across multiplex target enrichment workflows
Run overnight or same day workflows
Compromise no more.

"We recently adapted the Twist Exome 2.0 for our work for various indications, especially for unexplained developmental disorders. The content, performance and quality are the best we have experienced from any provider, and we didn’t have to choose between robust content and performance." - Oliver Wachter of the Center for Human Genetics and Laboratory Diagnostics in Germany (MVZ-Martinsried GmbH)

Detecting the Unexpected

Did you know that most commercially available exome panels cover nearly all gene-coding sequences in the genome? But, one key and critical differentiating factor is how deeply they cover specific genes. While some whole-exome panels focus on providing particularly deep coverage over genes that are relevant to cancer development, inherited disorders, or other such conditions, the Twist Exome 2.0 provides deep coverage over all genes that have been linked to clinical phenotypes, enabling translational research on a wide range of conditions. 

Learn More

Human Exome 2.0 Plus Comprehensive Exome Spike-in

Our latest human exome product spiked-in with all the Twist Comprehensive Exome targets that are omitted in Exome 2.0. You can now order this product, check resources tab to find SKU information.

Data
Best-in-Class Performance

Twist Exome 2.0 earns the title “best-in-class” by outperforming top competitors and enabling the most efficient exome sequencing. Compared to other exome panels, Twist Exome 2.0 delivers higher uniformity and a higher on-target rate as well as lower drop out and duplicate read rates. Together, this equates to fewer wasted reads and maximal coverage of target sequences. 

Twist Exome 2.0 earns the title “best-in-class” by outperforming top competitors and enabling the most efficient exome sequencing. Compared to other exome panels, Twist Exome 2.0 delivers higher uniformity and a higher on-target rate as well as lower drop out and duplicate read rates. Together, this equates to fewer wasted reads and maximal coverage of target sequences.
Most complete coverage of the human exome plus more

Twist Exome 2.0 features a complete new design that maximizes coverage of clinically relevant regions from the ClinVar database while including updated coverage of protein-coding regions from CCDS, RefSeq and GENCODE. In addition, the new design also includes pharmacogenomic SNPs, extended Tert promoter coverage, and 41 Sample ID SNPs for resolving contamination issues (Eurogentest, Pengally et al.).

Relative to competitors A, I, K, Twist Exome 2.0 provides the most comprehensive coding content coverage of target regions within the human exome and beyond. Twist Exome 2.0 demonstrated superior mean coverage of (a) protein-coding regions at depths of 30X, 40X & 50X; as well as superior coverage at 30x across (b) various important databases and gene lists.
Low GC bias enables target capture, regardless of GC content

Twist Exome 2.0 provides higher uniformity relative to competitors. Folded into this superior uniformity is a lower GC bias, meaning this panel can help you detect complex targets containing high, or low, levels of GC content. This means fewer rounds of sequencing are needed to adequately read targets with extreme GC content.

Twist Exome 2.0 demonstrates more uniform coverage over targets, regardless of their GC content, relative to competitors K, I, and A*.
Most efficient exome panel

Twist Exome 2.0’s superior uniformity, on-target rate, duplicate rate, and overall coverage translates into a highly efficient, cost-saving exome panel. This efficiency enables you to sequence more samples per run, or else to achieve deeper sequencing over desired targets.

Twist Exome 2.0 provides superior sequencing cost savings compared to competitors K, I, and A*. To demonstrate this, the amount of samples that could fit on a NovaSeq instrument S2 flow cell while still achieving 50x coverage over >80% of target bases was calculated for each exome panel.
Best-in-Class Performance

Twist Exome 2.0 earns the title “best-in-class” by outperforming top competitors and enabling the most efficient exome sequencing. Compared to other exome panels, Twist Exome 2.0 delivers higher uniformity and a higher on-target rate as well as lower drop out and duplicate read rates. Together, this equates to fewer wasted reads and maximal coverage of target sequences. 

Twist Exome 2.0 earns the title “best-in-class” by outperforming top competitors and enabling the most efficient exome sequencing. Compared to other exome panels, Twist Exome 2.0 delivers higher uniformity and a higher on-target rate as well as lower drop out and duplicate read rates. Together, this equates to fewer wasted reads and maximal coverage of target sequences.
Most complete coverage of the human exome plus more

Twist Exome 2.0 features a complete new design that maximizes coverage of clinically relevant regions from the ClinVar database while including updated coverage of protein-coding regions from CCDS, RefSeq and GENCODE. In addition, the new design also includes pharmacogenomic SNPs, extended Tert promoter coverage, and 41 Sample ID SNPs for resolving contamination issues (Eurogentest, Pengally et al.).

Relative to competitors A, I, K, Twist Exome 2.0 provides the most comprehensive coding content coverage of target regions within the human exome and beyond. Twist Exome 2.0 demonstrated superior mean coverage of (a) protein-coding regions at depths of 30X, 40X & 50X; as well as superior coverage at 30x across (b) various important databases and gene lists.
Low GC bias enables target capture, regardless of GC content

Twist Exome 2.0 provides higher uniformity relative to competitors. Folded into this superior uniformity is a lower GC bias, meaning this panel can help you detect complex targets containing high, or low, levels of GC content. This means fewer rounds of sequencing are needed to adequately read targets with extreme GC content.

Twist Exome 2.0 demonstrates more uniform coverage over targets, regardless of their GC content, relative to competitors K, I, and A*.
Most efficient exome panel

Twist Exome 2.0’s superior uniformity, on-target rate, duplicate rate, and overall coverage translates into a highly efficient, cost-saving exome panel. This efficiency enables you to sequence more samples per run, or else to achieve deeper sequencing over desired targets.

Twist Exome 2.0 provides superior sequencing cost savings compared to competitors K, I, and A*. To demonstrate this, the amount of samples that could fit on a NovaSeq instrument S2 flow cell while still achieving 50x coverage over >80% of target bases was calculated for each exome panel.
Workflow

Twist Exome 2.0 is designed to help you cover the target regions that are most important to you. To do this, we’ve built it to be amenable to a wide variety of customization options. If you’re studying calls for analysis of promoters, intronic SNPs, or UTRs, custom content can be quickly and affordably spiked into the panel to provide coverage. High-throughput studies are also enabled through sample multiplexing, up to 16-plex per reaction.

Whether you want to run the hybridization overnight or on the same day, the new Twist Standard Hyb v2 chemistry & Fast Hyb chemistry will accommodate your preference respectively.

 

Exome 2.0 Workflow

Twist Exome 2.0 is designed to help you cover the target regions that are most important to you. To do this, we’ve built it to be amenable to a wide variety of customization options. If you’re studying calls for analysis of promoters, intronic SNPs, or UTRs, custom content can be quickly and affordably spiked into the panel to provide coverage. High-throughput studies are also enabled through sample multiplexing, up to 16-plex per reaction.

Whether you want to run the hybridization overnight or on the same day, the new Twist Standard Hyb v2 chemistry & Fast Hyb chemistry will accommodate your preference respectively.

 

Exome 2.0 Workflow

Ordering and Specs
Product SKUs

104132

Twist Exome 2.0, 2 Reactions, Kit

104134

Twist Exome 2.0, 12 Reactions, Kit

104136

Twist Exome 2.0, 96 Reactions, Kit

105034

Twist Exome 2.0 plus Comprehensive Exome Spike-in, 2 Reactions

105035

Twist Exome 2.0 plus Comprehensive Exome Spike-in, 12 Reactions

105036

Twist Exome 2.0 plus Comprehensive Exome Spike-in, 96 Reactions
Product SKUs

104132

Twist Exome 2.0, 2 Reactions, Kit

104134

Twist Exome 2.0, 12 Reactions, Kit

104136

Twist Exome 2.0, 96 Reactions, Kit

105034

Twist Exome 2.0 plus Comprehensive Exome Spike-in, 2 Reactions

105035

Twist Exome 2.0 plus Comprehensive Exome Spike-in, 12 Reactions

105036

Twist Exome 2.0 plus Comprehensive Exome Spike-in, 96 Reactions
Resources
Detect the Unexpected
Detect the Unexpected
Detect the Unexpected