RNA LIBRARY PREP RNA EXOME RNA FUSION ORDERING RESOURCES
RNA Library Prep

Twist’s RNA products offer a complete NGS solution that produces uniform sequencing libraries for RNA sequencing. The end-to-end workflows achieve this by combining streamlined and effective library preparation kits, high quality unique dual indices (UDI), unique molecular identifiers, optimized target enrichment, and proprietary exon-aware panel design algorithm.

Twist Targeted RNA Sequencing Workflow

  • Create custom panels to sequence only RNA transcripts that matter
  • Combine Twist RNA Library Prep, Twist Hybridization and RNA target enrichment panels
  • Enrich sensitivity for key targets even in low quality, degraded RNA from FFPE

targeted

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Twist Whole Transcriptome Sequencing Workflow

  • Measure expression levels of mRNA, lncRNA and other RNA species
  • Prepare libraries from Total RNA extracted from fresh and FFPE samples
  • Combine our Twist RNA Library Prep with Twist rRNA & Globin Depletion kit
  • Perform RNA Library Preparation in less than 5 hours

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Related Resources

 

Twist’s RNA products offer a complete NGS solution that produces uniform sequencing libraries for RNA sequencing. The end-to-end workflows achieve this by combining streamlined and effective library preparation kits, high quality unique dual indices (UDI), unique molecular identifiers, optimized target enrichment, and proprietary exon-aware panel design algorithm.

Twist Targeted RNA Sequencing Workflow

  • Create custom panels to sequence only RNA transcripts that matter
  • Combine Twist RNA Library Prep, Twist Hybridization and RNA target enrichment panels
  • Enrich sensitivity for key targets even in low quality, degraded RNA from FFPE

targeted

chart

Twist Whole Transcriptome Sequencing Workflow

  • Measure expression levels of mRNA, lncRNA and other RNA species
  • Prepare libraries from Total RNA extracted from fresh and FFPE samples
  • Combine our Twist RNA Library Prep with Twist rRNA & Globin Depletion kit
  • Perform RNA Library Preparation in less than 5 hours

whole

chart 2

Related Resources

 

RNA Exome

RNA Exome

The Twist RNA Exome, along with Twist RNA Library Prep and Twist Target Enrichment, provides a reliable tool for generating transcriptome sequencing data from RNA extracted from a variety of sources including FFPE. RNA Exome increases the signal while requiring fewer sequencing reads for the same number of genes detect. This enables the detection of low expressing targets that are critical for an accurate picture of the transcriptional state of the biological sample. Additionally, the RNA Exome’s exon-aware design approach leaves open the ability to detect isoforms and junctions that may otherwise get lost with typical design approaches. This complete sequencing solution can produce highly complex and uniform sequencing reads for RNA sequencing analysis of the protein coding sequences of the human transcriptome.

Designed for the Human Transcriptome
Designed for the Human Transcriptome
Target 35.8 Mb bases, 19,708 genes and 63,215 isoforms
Built with sequences from Gencode and RefSeq
More than 1.8-fold enrichment over whole transcriptome
Target Enrichment Built for RNA
Target Enrichment Built for RNA
Exon-aware probe design for protein coding regions, fusions and isoforms
Fewer reads per sample for more samples
Compatible with FFPE and low RNA input

More Targets, Fewer Reads

Twist RNA Exome detects more targets with fewer reads over a range of RNA inputs with FFPE and universal human reference RNA. All libraries were prepared with the Twist RNA Library Prep.

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RNA Exome

The Twist RNA Exome, along with Twist RNA Library Prep and Twist Target Enrichment, provides a reliable tool for generating transcriptome sequencing data from RNA extracted from a variety of sources including FFPE. RNA Exome increases the signal while requiring fewer sequencing reads for the same number of genes detect. This enables the detection of low expressing targets that are critical for an accurate picture of the transcriptional state of the biological sample. Additionally, the RNA Exome’s exon-aware design approach leaves open the ability to detect isoforms and junctions that may otherwise get lost with typical design approaches. This complete sequencing solution can produce highly complex and uniform sequencing reads for RNA sequencing analysis of the protein coding sequences of the human transcriptome.

Designed for the Human Transcriptome
Designed for the Human Transcriptome
Target 35.8 Mb bases, 19,708 genes and 63,215 isoforms
Built with sequences from Gencode and RefSeq
More than 1.8-fold enrichment over whole transcriptome
Target Enrichment Built for RNA
Target Enrichment Built for RNA
Exon-aware probe design for protein coding regions, fusions and isoforms
Fewer reads per sample for more samples
Compatible with FFPE and low RNA input

More Targets, Fewer Reads

Twist RNA Exome detects more targets with fewer reads over a range of RNA inputs with FFPE and universal human reference RNA. All libraries were prepared with the Twist RNA Library Prep.

graph

RNA Fusion

RNA Fusion

Gene fusions often act as oncogenic driver mutations in a wide variety of cancer types. Their rapid and accurate detection can inform clinical actions and drive treatment decisions. The Twist Alliance CeGaT RNA Fusion Panel Kit is an RNA-based enrichment panel and offers an improved targeted and sensitive approach to investigate gene fusions than the conventional methods.

This panel includes 160 fusion genes associated with approximately 30 cancer types. The fusion genes were selected by CeGaT, a leading genetic diagnostic and sequencing company located in Europe. The design is not only for the detection of known gene fusions but is also used to identify novel gene fusions.

The panel is optimized and validated for use with the Twist RNA Library Prep Kit for a complete end-to-end workflow exceptional performance. High capture efficiency with the RNA-based enrichment increases the sensitivity of detection. This panel is ideal for screening oncology samples to inform treatment options or discover novel fusions for tumor classifications.*

target
Confidently Enrich RNA Gene Fusions
Expert content design through collaboration
with CeGaT
Optimized workflow with Twist RNA Library
Prep kit
Twist core enrichment technology maximizes capture efficiency

Panel Design

  • 160 fusion associated genes with 66 genes in the breakpoint design
  • 7394 Probes
  • Covers a variety of different cancer types

* Note: The Twist Alliance CeGaT RNA Fusion Panel – 3 MB is ISO-13485 certified.

Related Resources

Customer Testimonial

“At CeGaT, we want to offer comprehensive and affordable tumor diagnostics to our clients. The Twist Target Enrichment technology is a robust workflow allowing us to accurately analyze patient samples with more than 80 therapeutically actionable fusion variants in a high-throughput lab workflow. We can avoid allelic dropout issues associated with amplicon-based workflows. Based on our own validation, the panel allows us to report clinically actionable results and support treatment decisions.”**

Dr. Florian Battke, Director of Development, CeGaT GmbH

**Disclaimer: Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.

RNA Fusion

Gene fusions often act as oncogenic driver mutations in a wide variety of cancer types. Their rapid and accurate detection can inform clinical actions and drive treatment decisions. The Twist Alliance CeGaT RNA Fusion Panel Kit is an RNA-based enrichment panel and offers an improved targeted and sensitive approach to investigate gene fusions than the conventional methods.

This panel includes 160 fusion genes associated with approximately 30 cancer types. The fusion genes were selected by CeGaT, a leading genetic diagnostic and sequencing company located in Europe. The design is not only for the detection of known gene fusions but is also used to identify novel gene fusions.

The panel is optimized and validated for use with the Twist RNA Library Prep Kit for a complete end-to-end workflow exceptional performance. High capture efficiency with the RNA-based enrichment increases the sensitivity of detection. This panel is ideal for screening oncology samples to inform treatment options or discover novel fusions for tumor classifications.*

Customer Testimonial

“At CeGaT, we want to offer comprehensive and affordable tumor diagnostics to our clients. The Twist Target Enrichment technology is a robust workflow allowing us to accurately analyze patient samples with more than 80 therapeutically actionable fusion variants in a high-throughput lab workflow. We can avoid allelic dropout issues associated with amplicon-based workflows. Based on our own validation, the panel allows us to report clinically actionable results and support treatment decisions.”**

Dr. Florian Battke, Director of Development, CeGaT GmbH

**Disclaimer: Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.

target
Confidently Enrich RNA Gene Fusions
Expert content design through collaboration
with CeGaT
Optimized workflow with Twist RNA Library
Prep kit
Twist core enrichment technology maximizes capture efficiency

Panel Design

  • 160 fusion associated genes with 66 genes in the breakpoint design
  • 7394 Probes
  • Covers a variety of different cancer types

* Note: The Twist Alliance CeGaT RNA Fusion Panel – 3 MB is ISO-13485 certified.

Related Resources

Ordering
RNA Library Prep Product SKUS

107060

Twist RNA Library Prep Kit, 16 Samples

107061

Twist RNA Library Prep Kit, 96 Samples

107147

Twist RNA Library Prep with Depletion, 16 Samples

107148

Twist RNA Library Prep with Depletion, 96 Samples
RNA Exome Product SKUS

107143

RNA Exome 2 Reactions

107144

RNA Exome 12 Reactions

107146

RNA Exome 96 Reactions
RNA Fusion Product SKUS

106067

Twist Alliance CeGaT RNA Fusion Panel – 3 MB, 2 Rxn Kit

106101

Twist Alliance CeGaT RNA Fusion Panel – 3 MB, 12 Rxn Kit

106100

Twist Alliance CeGaT RNA Fusion Panel - 3 MB, 96 Rxn Kit
RNA Library Prep Product SKUS

107060

Twist RNA Library Prep Kit, 16 Samples

107061

Twist RNA Library Prep Kit, 96 Samples

107147

Twist RNA Library Prep with Depletion, 16 Samples

107148

Twist RNA Library Prep with Depletion, 96 Samples
RNA Exome Product SKUS

107143

RNA Exome 2 Reactions

107144

RNA Exome 12 Reactions

107146

RNA Exome 96 Reactions
RNA Fusion Product SKUS

106067

Twist Alliance CeGaT RNA Fusion Panel – 3 MB, 2 Rxn Kit

106101

Twist Alliance CeGaT RNA Fusion Panel – 3 MB, 12 Rxn Kit

106100

Twist Alliance CeGaT RNA Fusion Panel - 3 MB, 96 Rxn Kit
resources
Ease your RNA-Seq Workflow
Ease your RNA-Seq Workflow
Ease your RNA-Seq Workflow