Low bias, high fidelity amplification built for NGS Workflows.
Engineered for NGS library amplification, Twist TrueAmp Polymerase delivers uniform coverage across GC extremes, high sensitivity from low inputs, and automation ready hot start performance.
Unbiased, High Fidelity Results
Amplify uniformly across extreme 5–95% GC regions to maintain coverage of difficult targets. Improved fidelity and reduced homopolymer slippage deliver cleaner data and more confident calls with fewer repeats.
Reliable Performance from Challenging Samples
Generate consistent, high-yield libraries from complex samples—even at ≤100 pg input—so you recover more usable data and keep your workflows moving forward.
Simplified, Scalable Workflows
An aptamer enabled hot start enables room temperature stability for automation, while a single-tube 2× mastermix simplifies setup and reduces hands-on time; helping you scale workflows with fewer errors and faster turnaround.
Low bias, high fidelity amplification built for NGS Workflows.
Engineered for NGS library amplification, Twist TrueAmp Polymerase delivers uniform coverage across GC extremes, high sensitivity from low inputs, and automation ready hot start performance.
Unbiased, High Fidelity Results
Amplify uniformly across extreme 5–95% GC regions to maintain coverage of difficult targets. Improved fidelity and reduced homopolymer slippage deliver cleaner data and more confident calls with fewer repeats.
Reliable Performance from Challenging Samples
Generate consistent, high-yield libraries from complex samples—even at ≤100 pg input—so you recover more usable data and keep your workflows moving forward.
Simplified, Scalable Workflows
An aptamer enabled hot start enables room temperature stability for automation, while a single-tube 2× mastermix simplifies setup and reduces hands-on time; helping you scale workflows with fewer errors and faster turnaround.
Product data
Figure 1. GC-normalized coverage; AT-rich C. difficile and GC-rich B. pertussis.
Figure 2. Serial dilutions from 1 ng to 100 fg, Qubit and TapeStation QC.
Figure 3. Substitution rates after >7M base incorporations; homopolymer readout using Twist clonal plasmids.
Figure 4. Tolerance to Paramagnetic Beads During PCR. PCR reactions were spiked with 6.25 μl, 12.5 μl, 25 μl, and 50 μl of MyOne T1 Beads, M270 Beads (Invitrogen), and Twist DNA Purification Beads. Reactions were purified post bead-removal and quantified on Qubit dsDNA Broad Range Assay.
Engineered amplification that preserves library complexity
Many PCR enzymes are optimized for single-amplicon tasks (qPCR, cloning) rather than complex NGS libraries.
TrueAmp changes the approach
An engineered proofreading, high-processivity DNA polymerase that delivers best-in-class GC uniformity, yield, and fidelity for library amplification.
The result
Fewer biases toward short, GC-neutral molecules and more usable data per run.
Key features include
Single-tube 2× mastermix simplifies setup and reduces handling errors
Compatible with pre-capture and post-capture amplification
Designed for same-day or overnight hybridization workflows alongside Twist TE kits
Validated with Twist library prep & enrichment reagents to maintain end-to-end performance consistency
Product data
Figure 1. GC-normalized coverage; AT-rich C. difficile and GC-rich B. pertussis.
Figure 2. Serial dilutions from 1 ng to 100 fg, Qubit and TapeStation QC.
Figure 3. Substitution rates after >7M base incorporations; homopolymer readout using Twist clonal plasmids.
Figure 4. Tolerance to Paramagnetic Beads During PCR. PCR reactions were spiked with 6.25 μl, 12.5 μl, 25 μl, and 50 μl of MyOne T1 Beads, M270 Beads (Invitrogen), and Twist DNA Purification Beads. Reactions were purified post bead-removal and quantified on Qubit dsDNA Broad Range Assay.
Engineered amplification that preserves library complexity
Many PCR enzymes are optimized for single-amplicon tasks (qPCR, cloning) rather than complex NGS libraries.
TrueAmp changes the approach
An engineered proofreading, high-processivity DNA polymerase that delivers best-in-class GC uniformity, yield, and fidelity for library amplification.
The result
Fewer biases toward short, GC-neutral molecules and more usable data per run.
Key features include
Single-tube 2× mastermix simplifies setup and reduces handling errors
Compatible with pre-capture and post-capture amplification
Designed for same-day or overnight hybridization workflows alongside Twist TE kits
Validated with Twist library prep & enrichment reagents to maintain end-to-end performance consistency
Highlighted resources
Product Sheet
Protocol
Highlighted resources
Related Workflows and Applications
Target enrichment workflows with Twist deliver scalable, low bias, and highly uniform coverage across large and complex genomic regions; enabling broader discovery, higher sensitivity, and greater flexibility than amplicon or array based methods.
Twist offers several tools to support research into liquid biopsies and early cancer characterization.
Related Workflows and Applications
Target enrichment workflows with Twist deliver scalable, low bias, and highly uniform coverage across large and complex genomic regions; enabling broader discovery, higher sensitivity, and greater flexibility than amplicon or array based methods.
Twist offers several tools to support research into liquid biopsies and early cancer characterization.
