Don’t Settle For Less
Don’t Settle For Less
Don’t Settle For Less
OVERVIEW VIRUS DETECTION ORDERING AND SPECS RESOURCES
OVERVIEW

The recent Coronavirus pandemic has led to a rapid response from the viral research and therapeutics community around the world. These research teams need robust and reliable genomic tools to classify and characterize viral samples. Twist offers Research-Use Only (RUO) NGS target enrichment panels for the detection and characterization of SARS-CoV-2 virus. The panel targets about 30 kb viral genome with approximately 1,000 probes, designed against the SARS-CoV-2 genome (GenBank: MN908947.3).

detection
Highly sensitive detection
Detection of as little as 10 copies of viral material with a fold enrichment of >100,000
Sequencing
More sequence information
Coverage of > 99.9% of the genome at 1X or greater post enrichment
Ability to identify viral mutations
monitor
Use for environmental monitoring and surveillance
Assess viral evolution over time
Track strain origin and transmission patterns

The recent Coronavirus pandemic has led to a rapid response from the viral research and therapeutics community around the world. These research teams need robust and reliable genomic tools to classify and characterize viral samples. Twist offers Research-Use Only (RUO) NGS target enrichment panels for the detection and characterization of SARS-CoV-2 virus. The panel targets about 30 kb viral genome with approximately 1,000 probes, designed against the SARS-CoV-2 genome (GenBank: MN908947.3).

detection
Highly sensitive detection
Detection of as little as 10 copies of viral material with a fold enrichment of >100,000
Sequencing
More sequence information
Coverage of > 99.9% of the genome at 1X or greater post enrichment
Ability to identify viral mutations
monitor
Use for environmental monitoring and surveillance
Assess viral evolution over time
Track strain origin and transmission patterns
VIRUS DETECTION

NGS for Virus Detection

Next-generation sequencing (NGS) offers high-throughput, specific identification of infections in a variety of sample types including blood, nasal swab, feces, etc. In the case of viral infections, however, direct sequencing can be a challenge due to the extremely low concentration of the viruses of interest and the presence of high background from the host. Target capture using DNA-based hybridization probes to isolate specific sequences out of a mixed genomic sample can increase the sensitivity and specificity of NGS-based efforts.

High Sensitivity Through Efficient Design

Twist’s highly sensitive and accurate target capture enables efficient viral RNA detection. We demonstrate target enrichment’s ability to capture and detect the viral genome at varying numbers of viral copies spiked into human reference RNA. At high viral load (approximately 1 million copies), the complete genome is recovered even with only 25,000 reads. While at lower copy numbers, coverage of large portions of the viral template is maintained. No reads from the negative control map to the viral template even when using 8 million mapped reads, demonstrating our detection at 10 copies represents true capture and not background contamination. Find out more in the App Note.

 

NGS SARS-CoV-2 Research Panel Genome Coverage
Easy Workflow from Purified RNA to Sequencing

The Twist SARS-CoV-2 Research Panel is provided with a simple-to-follow protocol from purified RNA samples to sequencing with easily available components.

NGS SARS-CoV-2 Research Panel Workflow

NGS for Virus Detection

Next-generation sequencing (NGS) offers high-throughput, specific identification of infections in a variety of sample types including blood, nasal swab, feces, etc. In the case of viral infections, however, direct sequencing can be a challenge due to the extremely low concentration of the viruses of interest and the presence of high background from the host. Target capture using DNA-based hybridization probes to isolate specific sequences out of a mixed genomic sample can increase the sensitivity and specificity of NGS-based efforts.

High Sensitivity Through Efficient Design

Twist’s highly sensitive and accurate target capture enables efficient viral RNA detection. We demonstrate target enrichment’s ability to capture and detect the viral genome at varying numbers of viral copies spiked into human reference RNA. At high viral load (approximately 1 million copies), the complete genome is recovered even with only 25,000 reads. While at lower copy numbers, coverage of large portions of the viral template is maintained. No reads from the negative control map to the viral template even when using 8 million mapped reads, demonstrating our detection at 10 copies represents true capture and not background contamination. Find out more in the App Note.

 

NGS SARS-CoV-2 Research Panel Genome Coverage
Easy Workflow from Purified RNA to Sequencing

The Twist SARS-CoV-2 Research Panel is provided with a simple-to-follow protocol from purified RNA samples to sequencing with easily available components.

NGS SARS-CoV-2 Research Panel Workflow
Ordering and Specs
PRODUCT SKUS – RESEARCH PANEL
102016 Twist SARS-CoV-2 Research Panel, 2 Reactions, Kit
102017 Twist SARS-CoV-2 Research Panel, 12 Reactions, Kit
102018 Twist SARS-CoV-2 Research Panel, 96 Reactions, Kit

The Twist Synthetic SARS-CoV-2 RNA Controls are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

PRODUCT SKUS – RESEARCH PANEL
102016 Twist SARS-CoV-2 Research Panel, 2 Reactions, Kit
102017 Twist SARS-CoV-2 Research Panel, 12 Reactions, Kit
102018 Twist SARS-CoV-2 Research Panel, 96 Reactions, Kit

The Twist Synthetic SARS-CoV-2 RNA Controls are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

RESOURCES