High Throughput Library Prep
High Throughput Library Prep
High Throughput Library Prep
OVERVIEW DATA ORDERING RESOURCES
Overview

Preparing DNA sequencing libraries often involves complex workflows and only produces small batches of samples at a time. For genomic studies where hundreds of specimens are being evaluated, cost-effective tools that can be integrated with existing processes are essential.

The Twist 96-Plex Library Preparation kit enables high-throughput library construction for whole genome sequencing. The kit is purpose-built for use in high throughput applications, including industrial seed selection, cattle breeding, and rational drug design. With primer tunability for varying GC content and up to 960 samples per kit, the 96-Plex Library Preparation kit offers an optimal balance between scalability and robust library construction. 
 

Workflow

The Twist 96-Plex Library Preparation workflow starts with up to 96 individual samples in a plate. After the first random priming and barcoding step, all samples are pooled into a single tube for the rest of the process. An optional plate barcode can be added in the index read position to allow simultaneous sequencing of multiple sample plates.
 

Twist 96-plex Library Prep Workflow


Recommended Application

  • Low-pass sequencing
  • Industrial seed selection
  • Cattle breeding
  • Plasmid sequence verification

Kit Overview

The Twist 96-Plex Library Preparation kit contains all reagents needed to prepare double stranded libraries with full length adapters compatible with Illumina sequencing chemistries from 50 nanograms of high molecular weight input material. No additional primers, enzymes, or beads are required.

The kit is available in 960 sample format, containing sufficient materials for four or ten runs.
 

High throughput
High throughput
Up to 960 individually barcoded samples per kit
Highly multiplexed reactions for up to 96 samples at once
Efficient
Efficient
$10 per sample
No fragmentation, repair, or ligation steps necessary
Adaptable
Adaptable
Enables optimization for high- or low-GC content samples
Same kit can be used for multiple sample types
Economical solution for hundreds of samples

At just $10 per sample, the Twist 96-Plex Library Preparation kit scales up without breaking the bank.
Each preparation process produces 96 sequencer-ready libraries that seamlessly integrate with existing 
sequencing workflows.

Preparing DNA sequencing libraries often involves complex workflows and only produces small batches of samples at a time. For genomic studies where hundreds of specimens are being evaluated, cost-effective tools that can be integrated with existing processes are essential.

The Twist 96-Plex Library Preparation kit enables high-throughput library construction for whole genome sequencing. The kit is purpose-built for use in high throughput applications, including industrial seed selection, cattle breeding, and rational drug design. With primer tunability for varying GC content and up to 960 samples per kit, the 96-Plex Library Preparation kit offers an optimal balance between scalability and robust library construction. 
 

Workflow

The Twist 96-Plex Library Preparation workflow starts with up to 96 individual samples in a plate. After the first random priming and barcoding step, all samples are pooled into a single tube for the rest of the process. An optional plate barcode can be added in the index read position to allow simultaneous sequencing of multiple sample plates.
 

Twist 96-plex Library Prep Workflow


Recommended Application

  • Low-pass sequencing
  • Industrial seed selection
  • Cattle breeding
  • Plasmid sequence verification

Kit Overview

The Twist 96-Plex Library Preparation kit contains all reagents needed to prepare double stranded libraries with full length adapters compatible with Illumina sequencing chemistries from 50 nanograms of high molecular weight input material. No additional primers, enzymes, or beads are required.

The kit is available in 960 sample format, containing sufficient materials for four or ten runs.
 

High throughput
High throughput
Up to 960 individually barcoded samples per kit
Highly multiplexed reactions for up to 96 samples at once
Efficient
Efficient
$10 per sample
No fragmentation, repair, or ligation steps necessary
Adaptable
Adaptable
Enables optimization for high- or low-GC content samples
Same kit can be used for multiple sample types
Economical solution for hundreds of samples

At just $10 per sample, the Twist 96-Plex Library Preparation kit scales up without breaking the bank.
Each preparation process produces 96 sequencer-ready libraries that seamlessly integrate with existing 
sequencing workflows.

DATA
One kit for many sample types

GC content is variable with different organisms, which can cause varying biases in library preparation. The Twist 96-Plex Library Preparation kit supplies two primer sets, for high and low GC genomes. By using the primer set suitable for the organism being tested, users can tune the kit to fit their needs. 

Twist 96 Plex Library Prep GC Organism Comparison Chart
Consistent Libraries at Scale

Sequencing libraries prepared with the Twist 96-Plex Library Preparation kit display uniform read counts across each plate. Bead-based size selection evenly captures fragments within a specified range.

Twist 96-plex Unique Aligned Reads
One kit for many sample types

GC content is variable with different organisms, which can cause varying biases in library preparation. The Twist 96-Plex Library Preparation kit supplies two primer sets, for high and low GC genomes. By using the primer set suitable for the organism being tested, users can tune the kit to fit their needs. 

Twist 96 Plex Library Prep GC Organism Comparison Chart
Consistent Libraries at Scale

Sequencing libraries prepared with the Twist 96-Plex Library Preparation kit display uniform read counts across each plate. Bead-based size selection evenly captures fragments within a specified range.

Twist 96-plex Unique Aligned Reads
Ordering
Product SKUs

104950

Twist 96-Plex Library Preparation Kit, 10x96 samples

104951

Twist 96-Plex Library Preparation Kit, 4x96 samples
Product SKUs

104950

Twist 96-Plex Library Preparation Kit, 10x96 samples

104951

Twist 96-Plex Library Preparation Kit, 4x96 samples
RESOURCES