Genes
Oligo Pools
NGS
Variant Libraries
Antibodies
IVDR Solutions
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Uncover true biological signals in complex genomic samples
Maximize your detection sensitivity for low-frequency somatic variants in cell-free DNA (cfDNA) and genomic DNA (gDNA) samples. This system enables the generation of consensus reads to effectively filter out PCR-induced errors and artifacts. By providing unique molecular identifiers (UMI) and dual-indexed (UDI) primers, it supports high-accuracy variant calling for advanced research in oncology and other fields where precision is key.
Enhance rare variant detection
Use matched molecular identifiers to build consensus reads that reveal true biological signals in low-abundance samples.
Minimize sequencing artifacts
Distinguish true somatic mutations from PCR-induced errors and library preparation artifacts for higher confidence in variant calling.
Ensure scalable workflows
Support both cfDNA and gDNA inputs with automation-friendly 16- or 96-sample formats in a 96-well plate configuration.
Product data
The Twist UMI Adapter System integrates seamlessly into existing library preparation workflows as a drop-in replacement for Twist Universal Adapters. The 5-bp-matched UMI adapters are first ligated to the input molecules and subsequently PCR-amplified with Twist’s Unique Dual-Indexed primers. The resulting indexed libraries enable post-sequencing consensus building, which offers higher detection sensitivity than standard methods.
Twist's UMI Adapter System offers a tool for consensus building that removes errors from PCR duplicates.
Estimated per-base error rate for non-error-corrected reads (gray) and error-corrected reads (green) measured from target enrichment of a synthetic cfDNA standard. After sequencing, reads were downsampled to 20,000x coverage and error correction was performed using duplex collapse, requiring at least one read from each original strand.
Video
Flexible workflow
The Twist UMI Adapter System integrates seamlessly into existing library preparation workflows as a drop-in replacement for Twist Universal Adapters. The 5-bp-matched UMI adapters are first ligated to the input molecules and subsequently PCR-amplified with Twist’s Unique Dual-Indexed primers. The resulting indexed libraries enable post-sequencing consensus building, which offers higher detection sensitivity than standard methods.
Greater sensitivity in variant detection
Twist's UMI Adapter System offers a tool for consensus building that removes errors from PCR duplicates.
Estimated per-base error rate for non-error-corrected reads (gray) and error-corrected reads (green) measured from target enrichment of a synthetic cfDNA standard. After sequencing, reads were downsampled to 20,000x coverage and error correction was performed using duplex collapse, requiring at least one read from each original strand.
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Flexible workflow
The Twist UMI Adapter System integrates seamlessly into existing library preparation workflows as a drop-in replacement for Twist Universal Adapters. The 5-bp-matched UMI adapters are first ligated to the input molecules and subsequently PCR-amplified with Twist’s Unique Dual-Indexed primers. The resulting indexed libraries enable post-sequencing consensus building, which offers higher detection sensitivity than standard methods.
Greater sensitivity in variant detection
Twist's UMI Adapter System offers a tool for consensus building that removes errors from PCR duplicates.
Which adapter system is right for you?
Twist's Adapter Systems improve the performance of any 'T-A' overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. They reduce adapter dimer formation, increase yield, and can eliminate or minimize PCR—improving data quality while lowering sequencing costs. With broad compatibility, high multiplexing (up to 3,072 indexes), and ready-to-use formats, they scale effortlessly from discovery to high-throughput studies. Quality is in our DNA—All Twist adapters are functionally validated to ensure performance and reliability in all NGS applications.
Compare our adapter systems
UMI Adapter System
Full Length UDI Adapters
Universal Adapter System
HT Universal Adapter System
Adapter System - Trinity Freestyle
Best For
Improved NGS variant accuracy
PCR-free Whole Genome Sequencing
Most applications
High throughput applications
Used with the Trinity Fast Hyb Workflow
Contains
UMI Adapers; UDI Primers
Full Length UDI Adapters
Universal Adapters; UDI Primers
Universal Adapters; HT UDI Primers
Universal Adapters compatible with Trinity Freestyle Sequencing; UDI Primers
Unique indexes
16 or 384
1536
16 or 384
3072
384
Index length
10bp
12bp
10bp
12bp
10bp
Available formats
96-well plates
96-well plates
96-well plates
96- or 384-well plates
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Illumina
Element Cloudbreak Freestyle
Illumina
Element Cloudbreak Freestyle
Illumina
Element Cloudbreak Freestyle
Element Trinity Freestyle
Stay updated
UMI Adapter System
Best For
Improved NGS variant accuracy
Contains
UMI Adapers; UDI Primers
Unique indexes
16 or 384
Index length
10bp
Available formats
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Full Length UDI Adapters
Best For
PCR-free Whole Genome Sequencing
Contains
Full Length UDI Adapters
Unique indexes
1536
Index length
12bp
Available formats
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Universal Adapter System
Best For
PCR-free Whole Genome Sequencing
Contains
Full Length UDI Adapters
Unique indexes
1536
Index length
12bp
Available formats
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
HT Universal Adapter System
Best For
Most applications
Contains
Universal Adapters; UDI Primers
Unique indexes
16 or 384
Index length
10bp
Available formats
96-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Adapter System - Trinity Freestyle
Best For
High throughput applications
Contains
Universal Adapters; HT UDI Primers
Unique indexes
3072
Index length
12bp
Available formats
96- or 384-well plates
Sequencer Compatibility
Illumina
Element Cloudbreak Freestyle
Twist products are for research use only not for use in diagnostic procedures. Illumina and Element Cloudbreak are registered trademarks of their respective owners.
