Complete coverage, expanded discovery
Get the full picture with Whole Genome Sequencing.
Whole Genome Sequencing (WGS) analyzes an organism’s entire genome—both coding and non-coding regions—offering a complete view beyond just exome sequencing. By capturing variants in regulatory regions, introns, structural elements, and mitochondrial DNA, WGS enables insights into complex diseases, rare conditions, and traits not explained by coding sequences alone. As sequencing costs decline and analysis tools improve, WGS is becoming a powerful tool in both clinical diagnostics and genomic research.
Whole Genome
Sequencing VIEW OFFERINGS
BENEFITS
- Covers billions of bases for the complete genome
- Offers comprehensive coverage for broader examination of genome alterations, including novel variants in both coding and noncoding regions
- Readily enables retrospective analysis as new genetic biomarkers emerge
- Key applications include rare disease research, population genomics, and functional genomics
How it works
Whole Genome Sequencing Workflow
WGS requires the preparation of DNA libraries that go directly onto a sequencer - no target enrichment step is required. There are a myriad of ways NGS libraries can be prepared that optimize for different research goals: sample or analyte type, workflow efficiency, coverage requirements, etc. Twist offers a comprehensive suite of library preparation kits and accessory products, empowering you to tailor your WGS workflows to your unique needs.
Why choose us
Streamline WGS with Precision-Engineered Library Prep
Tailored Enzymes to Improve Your Workflow
Twist’s library preparation kits incorporate proprietary enzymes designed using advanced AI/ML modeling and our high-throughput DNA synthesis platform. This unique enzyme development pipeline ensures that each enzyme is custom-engineered to maximize performance in NGS workflows - ensuring you receive superior results.
Superior Performance for High-Quality Data
Get more insights from your data. Twist’s high-yield, high-complexity libraries provide exceptional sequencing depth, reduce duplicates, and improve genome coverage efficiency - even for difficult regions like GC-rich areas.
Flexible Workflows for Any Application
With a range of library preparation kits tailored for diverse research goals and accessories such as adapters to meet your unique needs, there’s a Twist kit to suit any application. Plus, our team of skilled field application scientists (FAS) is always ready to help optimize your workflow and ensure you get the data you need.
Complete coverage, expanded discovery
Get the full picture with Whole Genome Sequencing.
Whole Genome Sequencing (WGS) analyzes an organism’s entire genome—both coding and non-coding regions—offering a complete view beyond just exome sequencing. By capturing variants in regulatory regions, introns, structural elements, and mitochondrial DNA, WGS enables insights into complex diseases, rare conditions, and traits not explained by coding sequences alone. As sequencing costs decline and analysis tools improve, WGS is becoming a powerful tool in both clinical diagnostics and genomic research.
Whole Genome
Sequencing VIEW OFFERINGS
BENEFITS
- Covers billions of bases for the complete genome
- Offers comprehensive coverage for broader examination of genome alterations, including novel variants in both coding and noncoding regions
- Readily enables retrospective analysis as new genetic biomarkers emerge
- Key applications include rare disease research, population genomics, and functional genomics
How it works
Whole Genome Sequencing Workflow
WGS requires the preparation of DNA libraries that go directly onto a sequencer - no target enrichment step is required. There are a myriad of ways NGS libraries can be prepared that optimize for different research goals: sample or analyte type, workflow efficiency, coverage requirements, etc. Twist offers a comprehensive suite of library preparation kits and accessory products, empowering you to tailor your WGS workflows to your unique needs.
Why choose us
Streamline WGS with Precision-Engineered Library Prep
Tailored Enzymes to Improve Your Workflow
Twist’s library preparation kits incorporate proprietary enzymes designed using advanced AI/ML modeling and our high-throughput DNA synthesis platform. This unique enzyme development pipeline ensures that each enzyme is custom-engineered to maximize performance in NGS workflows - ensuring you receive superior results.
Superior Performance for High-Quality Data
Get more insights from your data. Twist’s high-yield, high-complexity libraries provide exceptional sequencing depth, reduce duplicates, and improve genome coverage efficiency - even for difficult regions like GC-rich areas.
Flexible Workflows for Any Application
With a range of library preparation kits tailored for diverse research goals and accessories such as adapters to meet your unique needs, there’s a Twist kit to suit any application. Plus, our team of skilled field application scientists (FAS) is always ready to help optimize your workflow and ensure you get the data you need.
What we offer
Featured WGS solutions
| Feature | 
Twist TrueAmp Library Prep Kit Ideal for Target Enrichment Workflows |
Twist PCR-Free WGS Library Kit Built for PCR Free Whole Genome Sequencing |
cfDNA Library Prep w/ TrueAmp Polymerase Highest sensitivity kit optimized for low-input and cfDNA |
FlexPrep UHT Library Prep Highest throughput option with a streamlined workflow |
| Overview | Ideal for Target Enrichment Workflows | Built for PCR Free Whole Genome Sequencing | Highest sensitivity kit optimized for low-input and cfDNA | Highest throughput option with a streamlined workflow |
| Fragmentation Process | Enzymatic | Enzymatic | Mechanical | Enzymatic |
| Throughput | Middle/High | Middle/High | Low | Highest |
| Input Range | 1-500 ng | 37.5-300 ng | ≤1 ng | 30–300 ng |
| Best For | Clinical/Research NGS | Clinical/Research NGS | Liquid biopsy | High-throughput studies (e.g., population genomics, agrigenomics) |
NEW
Introducing The Twist PCR-Free WGS Library Preparation Kit
Power your sequencing with Twist’s enzyme driven library prep; engineered to deliver more data from your sample.
What we offer
Explore the rest of the WGS lineup
Optimize your WGS workflow
Suggested accessories
Twist Full Length UDI Adapters
For high-throughput WGS requiring sample indexing, we recommend the Twist Full Length UDI Adapters. With up to 1,536 unique dual indexes (UDIs) available, these adapters allow for a streamlined, PCR-free workflow.
What we offer
Featured WGS solutions
| Feature |
Twist TrueAmp Library Prep Kit Ideal for Target Enrichment Workflows |
Twist PCR-Free WGS Library Kit Built for PCR Free Whole Genome Sequencing |
cfDNA Library Prep w/ TrueAmp Polymerase Highest sensitivity kit optimized for low-input and cfDNA |
FlexPrep UHT Library Prep Highest throughput option with a streamlined workflow |
| Overview | Ideal for Target Enrichment Workflows | Built for PCR Free Whole Genome Sequencing | Highest sensitivity kit optimized for low-input and cfDNA | Highest throughput option with a streamlined workflow |
| Fragmentation Process | Enzymatic | Enzymatic | Mechanical | Enzymatic |
| Throughput | Middle/High | Middle/High | Low | Highest |
| Input Range | 1-500 ng | 37.5-300 ng | ≤1 ng | 30–300 ng |
| Best For | Clinical/Research NGS | Clinical/Research NGS | Liquid biopsy | High-throughput studies (e.g., population genomics, agrigenomics) |
NEW
Introducing The Twist PCR-Free WGS Library Preparation Kit
Power your sequencing with Twist’s enzyme driven library prep; engineered to deliver more data from your sample.
What we offer
Explore the rest of the WGS lineup
Optimize your WGS workflow
Suggested accessories
Twist Full Length UDI Adapters
For high-throughput WGS requiring sample indexing, we recommend the Twist Full Length UDI Adapters. With up to 1,536 unique dual indexes (UDIs) available, these adapters allow for a streamlined, PCR-free workflow.
Twist products are for research use only. The products presented here are not intended for the diagnosis, prevention, or treatment of a disease or condition. Twist Bioscience assumes no liability regarding use of the product for applications in which it is not intended. The results are specific to the institution to which they were obtained. The results presented are customer-specific and should not be interpreted as indicative of performance across all institutions.
Twist products are for research use only. The products presented here are not intended for the diagnosis, prevention, or treatment of a disease or condition. Twist Bioscience assumes no liability regarding use of the product for applications in which it is not intended. The results are specific to the institution to which they were obtained. The results presented are customer-specific and should not be interpreted as indicative of performance across all institutions.
