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Complete coverage, expanded discovery

Get the full picture with Whole Genome Sequencing.

Whole Genome Sequencing (WGS) analyzes an organism’s entire genome—both coding and non-coding regions—offering a complete view beyond just exome sequencing. By capturing variants in regulatory regions, introns, structural elements, and mitochondrial DNA, WGS enables insights into complex diseases, rare conditions, and traits not explained by coding sequences alone. As sequencing costs decline and analysis tools improve, WGS is becoming a powerful tool in both clinical diagnostics and genomic research.

Whole Genome
Sequencing
BENEFITS
  • Covers billions of bases for the complete genome
  • Offers comprehensive coverage for broader examination of genome alterations, including novel variants in both coding and noncoding regions
  • Readily enables retrospective analysis as new genetic biomarkers emerge
  • Key applications include rare disease research, population genomics, and functional genomics
Whole Genome Sequencing Diagram
Whole Exome
Sequencing
BENEFITS
  • Targets only 1-2% of the entire genome, focusing on protein coding regions and regions containing known disease causing variants
  • Useful for routine variant determination
  • Enables greater depth of sequencing for detection of target variants with improved sensitivity
  • Reduces sequencing reads required substantially, enables greater throughput of samples per sequencing run
Whole Exome Sequencing Diagram
How it works

Whole Genome Sequencing Workflow

WGS requires the preparation of DNA libraries that go directly onto a sequencer - no target enrichment step is required. There are a myriad of ways NGS libraries can be prepared that optimize for different research goals: sample or analyte type, workflow efficiency, coverage requirements, etc. Twist offers a comprehensive suite of library preparation kits and accessory products, empowering you to tailor your WGS workflows to your unique needs.

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Why choose us

Streamline WGS with Precision-Engineered
Library Prep

.

detection-and-profiling
Tailored Enzymes to Improve Your Workflow

Twist’s library preparation kits incorporate proprietary enzymes designed using advanced AI/ML modeling and our high-throughput DNA synthesis platform. This unique enzyme development pipeline ensures that each enzyme is custom-engineered to maximize performance in NGS workflows - ensuring you receive superior results.
flexibility
Superior Performance for High-Quality Data

Get more insights from your data. Twist’s high-yield, high-complexity libraries provide exceptional sequencing depth, reduce duplicates, and improve genome coverage efficiency - even for difficult regions like GC-rich areas.
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Flexible Workflows for Any Application

With a range of library preparation kits tailored for diverse research goals and accessories such as adapters to meet your unique needs, there’s a Twist kit to suit any application. Plus, our team of skilled field application scientists (FAS) is always ready to help optimize your workflow and ensure you get the data you need.
What we offer

Featured WGS solutions

Enzymatic Fragmentation 2.0

Ideal for a wide range of workflows including PCR-Free WGS and target enrichment

PCR-Free WGS Library Prep Kit

Built for PCR-Free Whole Genome Sequencing

cFDNA Library Prep Kit

Highest sensitivity kit optimized for low-input and cfDNA

FlexPrep UHT Library Prep Kit

Streamlined library prep for ultra high-throughput workflows

Fragmentation Process

Enzymatic

Enzymatic

NA or Mechanical

Enzymatic

Throughput

Middle/High

Middle/High

Middle/High

Highest (UHT)

Input Range

75-500 ng

40-300 ng

≤1 ng

30–300 ng

Best For

Clinical/Research NGS

Clinical/Research NGS. Rare diseases and cancer research

Liquid biopsy

High-throughput studies (e.g., population genomics, agrigenomics)

Learn more
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Enzymatic Fragmentation 2.0

Ideal for a wide range of workflows including PCR-Free WGS and target enrichment

Fragmentation Process

Enzymatic

Throughput

Middle/High

Input Range

75-500 ng

Best For

Clinical/Research NGS

PCR-Free WGS Library Prep Kit

Built for PCR-Free Whole Genome Sequencing

Fragmentation Process

Enzymatic

Throughput

Middle/High

Input Range

40-300 ng

Best For

Clinical/Research NGS. Rare diseases and cancer research

cFDNA Library Prep Kit

Highest sensitivity kit optimized for low-input and cfDNA

Enzymatic

Middle/High

40-300 ng

Clinical/Research NGS. Rare diseases and cancer research

FlexPrep UHT Library Prep Kit

Streamlined library prep for ultra high-throughput workflows

NA or Mechanical

Middle/High

≤1 ng

Liquid biopsy

NEW

Introducing The Twist PCR-Free WGS Library Preparation Kit

Power your sequencing with Twist’s enzyme driven library prep; engineered to deliver more data from your sample.

LEARN MORE
What we offer

Explore the rest of the WGS lineup

 

Optimize your WGS workflow

Suggested accessories

population
Highlighted applications

Population Genomics

WGS enables comprehensive analysis of genetic variation across human populations, supporting studies in diversity, adaptation, ancestry, and disease association. Twist’s high-throughput WGS solutions are ideal for large-cohort research, enabling precise variant detection and uniform coverage critical for trait mapping and population-scale insights.

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Twist products are for research use only. The products presented here are not intended for the diagnosis, prevention, or treatment of a disease or condition. Twist Bioscience assumes no liability regarding use of the product for applications in which it is not intended. The results are specific to the institution to which they were obtained. The results presented are customer-specific and should not be interpreted as indicative of performance across all institutions.

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681 Gateway Blvd
South San Francisco, CA 94080