Standard Hybridization Solution v2

OVERVIEW DATA RESOURCES

OVERVIEW

The Twist Standard Hybridization Reagent Kit v2 is the gold standard for efficient and specific binding of panel probes to your regions of interest. Coupled with Twist’s Catalog or Custom Panels, this standard hybridization workflow provides the optimal conditions for a broad range of content.

Engineered specifically for NGS library amplification (pre- and post-capture), Twist TrueAmp Polymerase Mix delivers uniform coverage across challenging GC extremes, high sensitivity from low input, and automation-ready hot-start control—all in a simple 2× mastermix. This leads to unbiased, high-fidelity results and reliable performance from challenging samples with a simplified and scalable workflow.

KEY BENEFITS

Optimum Coverage

Delivers excellent uniformity and increased on-target rates

Fully automatable

Cost-efficient, even coverage

Accommodates a Broad Range of Panels

Standardized conditions require no optimization

Compatible with fixed content, custom, or spike-ins

TrueAmp Polymerase Optimization

Amplify without bias across 5%–95% GC to maintain visibility of difficult targets.

Consistent high yields from complex libraries—even at ≤100 pg DNA input

Call variants with confidence via improved fidelity and reduced homopolymer slippage.

Scale with ease using an aptamer-enabled hot-start; inhibits both polymerase and exonuclease activity at room temperature for on-deck stability.

Streamline workflows with a single-tube 2× mastermix, validated with Twist library prep and target enrichment reagents.

DOWNLOADS

Twist Target Enrichment with TrueAmp Polymerase Protocol
Twist Target Enrichment Protocol: For use with the Twist NGS Workflow

DOWNLOADS

Twist Target Enrichment with TrueAmp Polymerase Protocol
Twist Target Enrichment Protocol: For use with the Twist NGS Workflow

KEY BENEFITS

Optimum Coverage

Delivers excellent uniformity and increased on-target rates

Fully automatable

Cost-efficient, even coverage

Accommodates a Broad Range of Panels

Standardized conditions require no optimization

Compatible with fixed content, custom, or spike-ins

TrueAmp Polymerase Optimization

Amplify without bias across 5%–95% GC to maintain visibility of difficult targets.

Consistent high yields from complex libraries—even at ≤100 pg DNA input

Call variants with confidence via improved fidelity and reduced homopolymer slippage.

Scale with ease using an aptamer-enabled hot-start; inhibits both polymerase and exonuclease activity at room temperature for on-deck stability.

Streamline workflows with a single-tube 2× mastermix, validated with Twist library prep and target enrichment reagents.

DATA

Optimized Out of the Box

Coverage obtained with Twist Core Exome (top panels) and coverage with the Twist Core Exome + exon spike-ins. Both conditions provided optimal coverage without changes to the hybridization conditions using the standard Hybridization and Wash Kit.

Maximize sequencing efficiency with even coverage

Even coverage throughout the target results in the most cost-efficient sequencing, and can increase sequencing throughput with higher multiplexing capabilities. Or use this extra sequencing flow cell space to get greater depth on the same number of samples.