RNA-Seq Library Preparation Overview
Twist RNA library preparation offers workflow which was optimized to take a sample through to completed library in as little as 5 hours (left). The whole transcriptome libraries can be sequenced directly for comprehensive analysis. They may also be used as input into Twist RNA target enrichment workflow with our Target Enrichment panels for RNA Exome, RNA Fusion, or custom designed RNA enrichment panels which focus NGS coverage on coding regions enabling gene expression analysis, fusion detection, and identification of coding variants from a single assay (right).
RNA-Seq Library Preparation Workflow
Starting with total RNA as input from 1 ng up to 1 ug of input can be introduced into the workflow with or without the optional ribosomal RNA and Globin depletion module to remove unwanted RNA species. The RNA is fragmented and then reverse transcribed into cDNA followed by a single-step 2nd strand synthesis and A-tailing that saves time and procedure steps. Finally, libraries are constructed by ligating Twist high quality UMIs or Universal Adaptors (UDIs) followed by amplification.
This product sheet highlights the Twist RNA Exome's capabilities, providing essential information for superior genomic sequencing outcomes.
This protocol details the steps to build sequencing libraries for target enrichment including converting RNA and preparing cDNA libraries with Twist UMIs
This protocol details the steps to build sequencing libraries for target enrichment including converting RNA and preparing cDNA libraries with Twist UDIs.
RNA-Seq Library Preparation Overview
Twist RNA library preparation offers workflow which was optimized to take a sample through to completed library in as little as 5 hours (left). The whole transcriptome libraries can be sequenced directly for comprehensive analysis. They may also be used as input into Twist RNA target enrichment workflow with our Target Enrichment panels for RNA Exome, RNA Fusion, or custom designed RNA enrichment panels which focus NGS coverage on coding regions enabling gene expression analysis, fusion detection, and identification of coding variants from a single assay (right).
RNA-Seq Library Preparation Workflow
Starting with total RNA as input from 1 ng up to 1 ug of input can be introduced into the workflow with or without the optional ribosomal RNA and Globin depletion module to remove unwanted RNA species. The RNA is fragmented and then reverse transcribed into cDNA followed by a single-step 2nd strand synthesis and A-tailing that saves time and procedure steps. Finally, libraries are constructed by ligating Twist high quality UMIs or Universal Adaptors (UDIs) followed by amplification.
This product sheet highlights the Twist RNA Exome's capabilities, providing essential information for superior genomic sequencing outcomes.
This protocol details the steps to build sequencing libraries for target enrichment including converting RNA and preparing cDNA libraries with Twist UMIs
This protocol details the steps to build sequencing libraries for target enrichment including converting RNA and preparing cDNA libraries with Twist UDIs.
RNA Exome
The Twist RNA Exome, along with Twist RNA Library Prep and Twist Target Enrichment, provides a reliable tool for generating transcriptome sequencing data from RNA extracted from a variety of sources including FFPE. RNA Exome increases the signal while requiring fewer sequencing reads for the same number of genes detect. This enables the detection of low expressing targets that are critical for an accurate picture of the transcriptional state of the biological sample. Additionally, the RNA Exome’s exon-aware design approach leaves open the ability to detect isoforms and junctions that may otherwise get lost with typical design approaches. This complete sequencing solution can produce highly complex and uniform sequencing reads for RNA sequencing analysis of the protein coding sequences of the human transcriptome.
More Targets, Fewer Reads
Twist RNA Exome detects more targets with fewer reads over a range of RNA inputs with FFPE and universal human reference RNA. All libraries were prepared with the Twist RNA Library Prep.
This poster highlights key advancements in RNA exome sequencing, offering detailed methodologies and research breakthroughs.
Discover insights from this application note on a targeted RNA sequencing workflow, designed to optimize genomic research outcomes.
This product sheet highlights the Twist RNA Exome's capabilities, providing essential information for superior genomic sequencing outcomes.
RNA Exome
The Twist RNA Exome, along with Twist RNA Library Prep and Twist Target Enrichment, provides a reliable tool for generating transcriptome sequencing data from RNA extracted from a variety of sources including FFPE. RNA Exome increases the signal while requiring fewer sequencing reads for the same number of genes detect. This enables the detection of low expressing targets that are critical for an accurate picture of the transcriptional state of the biological sample. Additionally, the RNA Exome’s exon-aware design approach leaves open the ability to detect isoforms and junctions that may otherwise get lost with typical design approaches. This complete sequencing solution can produce highly complex and uniform sequencing reads for RNA sequencing analysis of the protein coding sequences of the human transcriptome.
More Targets, Fewer Reads
Twist RNA Exome detects more targets with fewer reads over a range of RNA inputs with FFPE and universal human reference RNA. All libraries were prepared with the Twist RNA Library Prep.
This poster highlights key advancements in RNA exome sequencing, offering detailed methodologies and research breakthroughs.
Discover insights from this application note on a targeted RNA sequencing workflow, designed to optimize genomic research outcomes.
This product sheet highlights the Twist RNA Exome's capabilities, providing essential information for superior genomic sequencing outcomes.
RNA Fusion
Gene fusions often act as oncogenic driver mutations in a wide variety of cancer types. Their rapid and accurate detection can inform clinical actions and drive treatment decisions. The Twist Alliance CeGaT RNA Fusion Panel Kit is an RNA-based enrichment panel and offers an improved targeted and sensitive approach to investigate gene fusions than the conventional methods.
This panel includes 160 fusion genes associated with approximately 30 cancer types. The fusion genes were selected by CeGaT, a leading genetic diagnostic and sequencing company located in Europe. The design is not only for the detection of known gene fusions but is also used to identify novel gene fusions.
The panel is optimized and validated for use with the Twist RNA Library Prep Kit for a complete end-to-end workflow exceptional performance. High capture efficiency with the RNA-based enrichment increases the sensitivity of detection. This panel is ideal for screening oncology samples to inform treatment options or discover novel fusions for tumor classifications.*
Panel Design
- 160 fusion associated genes with 66 genes in the breakpoint design
- 7394 Probes
- Covers a variety of different cancer types
* Note: The Twist Alliance CeGaT RNA Fusion Panel – 3 MB is ISO-13485 certified.
Experience accurate and efficient RNA fusion detection with the Twist and CeGaT RNA Fusion Panel, designed for cutting-edge cancer research.
Enhance your research with the Twist CeGaT RNA Fusion Panel, delivering accurate RNA fusion detection for advanced cancer studies.
See how an RNA-based workflow paired with molecular analysis of tumor-derived samples can provide a deeper understanding of tumor biology
What Scientists Have to Say
RNA Fusion
At CeGaT, we want to offer comprehensive and affordable tumor diagnostics to our clients. The Twist Target Enrichment technology is a robust workflow allowing us to accurately analyze patient samples with more than 80 therapeutically actionable fusion variants in a high-throughput lab workflow. We can avoid allelic dropout issues associated with amplicon-based workflows. Based on our own validation, the panel allows us to report clinically actionable results and support treatment decisions.**
Dr. Florian Battke, Director of Development, CeGaT GmbH
**Disclaimer: Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.
RNA Fusion
Gene fusions often act as oncogenic driver mutations in a wide variety of cancer types. Their rapid and accurate detection can inform clinical actions and drive treatment decisions. The Twist Alliance CeGaT RNA Fusion Panel Kit is an RNA-based enrichment panel and offers an improved targeted and sensitive approach to investigate gene fusions than the conventional methods.
This panel includes 160 fusion genes associated with approximately 30 cancer types. The fusion genes were selected by CeGaT, a leading genetic diagnostic and sequencing company located in Europe. The design is not only for the detection of known gene fusions but is also used to identify novel gene fusions.
The panel is optimized and validated for use with the Twist RNA Library Prep Kit for a complete end-to-end workflow exceptional performance. High capture efficiency with the RNA-based enrichment increases the sensitivity of detection. This panel is ideal for screening oncology samples to inform treatment options or discover novel fusions for tumor classifications.*
Panel Design
- 160 fusion associated genes with 66 genes in the breakpoint design
- 7394 Probes
- Covers a variety of different cancer types
* Note: The Twist Alliance CeGaT RNA Fusion Panel – 3 MB is ISO-13485 certified.
What Scientists Have to Say
RNA Fusion
At CeGaT, we want to offer comprehensive and affordable tumor diagnostics to our clients. The Twist Target Enrichment technology is a robust workflow allowing us to accurately analyze patient samples with more than 80 therapeutically actionable fusion variants in a high-throughput lab workflow. We can avoid allelic dropout issues associated with amplicon-based workflows. Based on our own validation, the panel allows us to report clinically actionable results and support treatment decisions.**
Dr. Florian Battke, Director of Development, CeGaT GmbH
**Disclaimer: Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions.
Experience accurate and efficient RNA fusion detection with the Twist and CeGaT RNA Fusion Panel, designed for cutting-edge cancer research.
Enhance your research with the Twist CeGaT RNA Fusion Panel, delivering accurate RNA fusion detection for advanced cancer studies.
See how an RNA-based workflow paired with molecular analysis of tumor-derived samples can provide a deeper understanding of tumor biology
Blog
Poster
Product Sheet
Application Note
Application Note
Evaluation of a targeted RNA sequencing workflow for variant calling from FFPE samples in NSCLC
Technical Note
Data Files
Safety Data Sheet
Protocol
Protocol
Twist rRNA and Globin Depletion and RNA Library Preparation with the UMI Adapter System
Protocol
Twist rRNA and Globin Depletion and RNA Library Preparation with the Universal Adapter System
Application Note
Evaluation of a targeted RNA sequencing workflow for variant calling from FFPE samples in NSCLC
Protocol
Twist rRNA and Globin Depletion and RNA Library Preparation with the UMI Adapter System
Protocol
Twist rRNA and Globin Depletion and RNA Library Preparation with the Universal Adapter System
