Trusted, accurate and safe controls for
a wide-range of applications
The recent Coronavirus pandemic has led to the unprecedented need for diagnostic tests for detecting the presence of SARS-CoV-2 and other Respiratory viruses in a variety of sample types. To address this need, laboratories around the world need high quality tools to enable them in catering to this rapidly expanding requirement for testing.
Reproducible, Quality Results
Positive controls provide quality control measures for a wide range of applications from diagnostic assay development to day-to-day testing. This includes the verification and validation of diagnostic tests of both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays.
A Safe and Effective Alternative to “Live Virus”
Controls based on viral nucleic acids extracted from either an infected patient or from live virus propagated in cell culture have safety and security concerns. Synthetic controls created through gene synthesis broaden access across diverse strains while mitigating risk.
Two new controls have been added that include the new variant strain of SARS-CoV-2 (VoC-202012/01, B.1.1.7 lineage) identified in the United Kingdom.
This new strain contains multiple mutations in the spike protein and other areas of the viral genome.
Some RT-PCR probes used widely in SARS-CoV-2 testing detect the S gene that codes for the spike protein.
Our new controls will help you avoid false negative results when screening the new strain.
Hear how these new products are making a difference in testing, surveillance, vaccine development and therapeutic antibody discoveries.
Trusted, accurate and safe controls for
a wide-range of applications
The recent Coronavirus pandemic has led to the unprecedented need for diagnostic tests for detecting the presence of SARS-CoV-2 and other Respiratory viruses in a variety of sample types. To address this need, laboratories around the world need high quality tools to enable them in catering to this rapidly expanding requirement for testing.
Reproducible, Quality Results
Positive controls provide quality control measures for a wide range of applications from diagnostic assay development to day-to-day testing. This includes the verification and validation of diagnostic tests of both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays.
A Safe and Effective Alternative to “Live Virus”
Controls based on viral nucleic acids extracted from either an infected patient or from live virus propagated in cell culture have safety and security concerns. Synthetic controls created through gene synthesis broaden access across diverse strains while mitigating risk.
Two new controls have been added that include the new variant strain of SARS-CoV-2 (VoC-202012/01, B.1.1.7 lineage) identified in the United Kingdom.
This new strain contains multiple mutations in the spike protein and other areas of the viral genome.
Some RT-PCR probes used widely in SARS-CoV-2 testing detect the S gene that codes for the spike protein.
Our new controls will help you avoid false negative results when screening the new strain.
Hear how these new products are making a difference in testing, surveillance, vaccine development and therapeutic antibody discoveries.
Twist has created synthetic RNA controls from seven sequence variants of the SARS-CoV-2 virus. These variants were selected to cover a wide range of sequence diversity. Each control consists of six non-overlapping 5 kb fragments generated from Twist Gene Fragments then transcribed into ssRNA. These controls provide coverage of greater than 99.9% of the bases of the viral genome and are supplied in 100 μL at a concentration of approximately one million copies per microliter.
GenBank IDs and GIDAID names for synthetic controls 1 through 15.
As the pandemic continues to evolve and as new strains emerge, we’ll continue to expand this panel of synthetic controls to ensure you have the most up-to-date tools to track viral progression and evolution.
| Control | GENBANK ID | GISAID Name |
|---|---|---|
| Control 1 | MT007544.1 | Australia/VIC01/2020 |
| Control 2 | MN908947.3 | Wuhan-Hu-1 |
| Control 3 | LC528232.1 | Japan/Hu_DP_Kng_19-020/2020 |
| Control 4 | MT106054.1 | USA/TX1/2020 |
| Control 5 | MT188340 | USA/MN2-MDH2/2020 |
| Control 6 | MT118835 | USA/CA9/2020 |
| Control 7 | EPI_ISL_418227 | France/HF2393/2020 |
| Control 8 | MT066176 | Taiwan/NTU02/2020 |
| Control 9 | MT152824 | USA/WA2/2020 |
| Control 10 | EPI_ISL_414648 | USA/CA-PC101P/2020 |
| Control 11 | EPI_ISL_417739 | Iceland/5/2020 |
| Control 12 | EPI_ISL_420244 | England/SHEF-C05B2/2020 |
| Control 13 | EPI_ISL_421184 | Belgium/ULG-10004/2020 |
| Control 14 | EPI_ISL_710528 | England/205041766/2020 |
| Control 15 | EPI_ISL_601443 | England/MILK-9E05B3/2020 |
Twist has created synthetic RNA controls from seven sequence variants of the SARS-CoV-2 virus. These variants were selected to cover a wide range of sequence diversity. Each control consists of six non-overlapping 5 kb fragments generated from Twist Gene Fragments then transcribed into ssRNA. These controls provide coverage of greater than 99.9% of the bases of the viral genome and are supplied in 100 μL at a concentration of approximately one million copies per microliter.
GenBank IDs and GIDAID names for synthetic controls 1 through 15.
As the pandemic continues to evolve and as new strains emerge, we’ll continue to expand this panel of synthetic controls to ensure you have the most up-to-date tools to track viral progression and evolution.
| Control | GENBANK ID | GISAID Name |
|---|---|---|
| Control 1 | MT007544.1 | Australia/VIC01/2020 |
| Control 2 | MN908947.3 | Wuhan-Hu-1 |
| Control 3 | LC528232.1 | Japan/Hu_DP_Kng_19-020/2020 |
| Control 4 | MT106054.1 | USA/TX1/2020 |
| Control 5 | MT188340 | USA/MN2-MDH2/2020 |
| Control 6 | MT118835 | USA/CA9/2020 |
| Control 7 | EPI_ISL_418227 | France/HF2393/2020 |
| Control 8 | MT066176 | Taiwan/NTU02/2020 |
| Control 9 | MT152824 | USA/WA2/2020 |
| Control 10 | EPI_ISL_414648 | USA/CA-PC101P/2020 |
| Control 11 | EPI_ISL_417739 | Iceland/5/2020 |
| Control 12 | EPI_ISL_420244 | England/SHEF-C05B2/2020 |
| Control 13 | EPI_ISL_421184 | Belgium/ULG-10004/2020 |
| Control 14 | EPI_ISL_710528 | England/205041766/2020 |
| Control 15 | EPI_ISL_601443 | England/MILK-9E05B3/2020 |
The Twist Synthetic Respiratory Virus Controls cover a broad range of RNA and DNA viruses relevant to respiratory disease research. These controls are aligned with the content of the Twist Respiratory Virus Research Panel (PN 103067) and are suitable for use with Twist Fixed Panel NGS workflows or for use with RT-PCR or qPCR experiments of your own design.
The figure shows a taxonomic tree of viruses covered by the Twist Respiratory Virus Controls. The table shows GenBank IDs, virus type and length for each control. Additional viral controls may be available for custom synthesis. Please contact your local sales representative for more details.
Virus name Genbank IDs, virus type, and length for each virus included in the Twist Respiratory Virus Controls. Additional viral controls may be available for custom synthesis.
Contact your local sales representative for more details or Buy Now.
The Twist Synthetic Respiratory Virus Controls cover a broad range of RNA and DNA viruses relevant to respiratory disease research. These controls are aligned with the content of the Twist Respiratory Virus Research Panel (PN 103067) and are suitable for use with Twist Fixed Panel NGS workflows or for use with RT-PCR or qPCR experiments of your own design.
The figure shows a taxonomic tree of viruses covered by the Twist Respiratory Virus Controls. The table shows GenBank IDs, virus type and length for each control. Additional viral controls may be available for custom synthesis. Please contact your local sales representative for more details.
Virus name Genbank IDs, virus type, and length for each virus included in the Twist Respiratory Virus Controls. Additional viral controls may be available for custom synthesis.
Contact your local sales representative for more details or Buy Now.
The Twist Synthetic Viral Controls are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
The Twist Synthetic Viral Controls are for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
Application Note
NGS Target Enrichment of Viral Pathogens using Twist Respiratory Virus Research Panel
Application Note
NGS Target Enrichment of Viral Pathogens using Twist Respiratory Virus Research Panel
