Overview
Humanized Transgenic (HuTg) Mice
With proprietary mouse platforms comes a licensing process filled with legal fees, MTAs, royalty agreements, and restrictions. These hurdles can drain time and resources before R&D teams can begin in vivo discovery campaigns. Our humanized transgenic (HuTg) mouse platform is license-free, removing burdensome administrative barriers and supporting your path to discovery.
HuTg and DiversimAb – Better Together
Wild type, knockout and humanized in vivo platforms typically yield limited epitopic diversity, meaning fewer chances to uncover promising hits. To circumvent this, we offer to pair our HuTg mice with DiversimAb mice – a platform that generates a more diverse antibody repertoire – boosting epitope coverage, functional candidates and your chances of success.
HUTG MICE OFFERED BY TWIST |
DIVERSIMAB HYPERIMMUNE MICE |
Other HuTg MICE |
|
|---|---|---|---|
| Licensing Required? | No | No | Yes |
| Epitope Coverage and Diversity | Med | High | Low |
| Affinity Range | sub nM+ | sub nM+ | sub nM+ |
| Binders to high homology targets or epitopes? | No | Yes | No |
| Rapid (3 week) immunization available? | Yes | Yes | No |
| Performance with Beacon Single B Cell Screening | Good | Excellent | Poor |
| Humanization Required? | No | Yes | No |
| Risk of Developability Liabilities | Low | Low | Low |
| Cost | Med | Low | High |
Overview
Humanized Transgenic (HuTg) Mice
With proprietary mouse platforms comes a licensing process filled with legal fees, MTAs, royalty agreements, and restrictions. These hurdles can drain time and resources before R&D teams can begin in vivo discovery campaigns. Our humanized transgenic (HuTg) mouse platform is license-free, removing burdensome administrative barriers and supporting your path to discovery.
HuTg and DiversimAb – Better Together
Wild type, knockout and humanized in vivo platforms typically yield limited epitopic diversity, meaning fewer chances to uncover promising hits. To circumvent this, we offer to pair our HuTg mice with DiversimAb mice – a platform that generates a more diverse antibody repertoire – boosting epitope coverage, functional candidates and your chances of success.
HUTG MICE OFFERED BY TWIST |
DIVERSIMAB HYPERIMMUNE MICE |
Other HuTg MICE |
|
|---|---|---|---|
| Licensing Required? | No | No | Yes |
| Epitope Coverage and Diversity | Med | High | Low |
| Affinity Range | sub nM+ | sub nM+ | sub nM+ |
| Binders to high homology targets or epitopes? | No | Yes | No |
| Rapid (3 week) immunization available? | Yes | Yes | No |
| Performance with Beacon Single B Cell Screening | Good | Excellent | Poor |
| Humanization Required? | No | Yes | No |
| Risk of Developability Liabilities | Low | Low | Low |
| Cost | Med | Low | High |
Benefits
Humanized Mice: A Pathway to Preclinical Success
HuTg mice have been the origin for several commercially successful therapeutic antibodies. As in vivo antibody discovery evolves, humanized mouse platforms that generate robust immune responses and human antibody repertoires are key to reducing immunogenicity, accelerating lead optimization, and streamlining translation to the clinic.
Easing the Burden of Human Antibody Discovery
Our HuTg mice empowers your in-house antibody discovery efforts with faster timelines and at lower upfront costs, without the administrative red tape.
License-Free Access
Skip the paperwork and negotiations required by other humanized platforms. Our HuTg mice are ready to go, license-free.
High Titers, High Affinities
HuTg mice are immunized using an optimized, rapid or extended protocol to produce high titers of antigen-specific B cells and affinities in the sub-nM range (and up).
Fee-For-Service
No licensing, tech transfer, milestone or royalty fees required. We offer predictable pricing that supports faster and more accessible discovery.
Flexible Discovery
Maximize epitope coverage and de-risk your HuTg mice discovery campaign by integrating DiversimAb mice for expanded antibody diversity.
Want to learn more about Twist's antibody discovery services?
Connect with one of our experts to develop a tailored project proposal to support your specific research needs.
Benefits
Humanized Mice: A Pathway to Preclinical Success
HuTg mice have been the origin for several commercially successful therapeutic antibodies. As in vivo antibody discovery evolves, humanized mouse platforms that generate robust immune responses and human antibody repertoires are key to reducing immunogenicity, accelerating lead optimization, and streamlining translation to the clinic.
Easing the Burden of Human Antibody Discovery
Our HuTg mice empowers your in-house antibody discovery efforts with faster timelines and at lower upfront costs, without the administrative red tape.
License-Free Access
Skip the paperwork and negotiations required by other humanized platforms. Our HuTg mice are ready to go, license-free.
High Titers, High Affinities
HuTg mice are immunized using an optimized, rapid or extended protocol to produce high titers of antigen-specific B cells and affinities in the sub-nM range (and up).
Fee-For-Service
No licensing, tech transfer, milestone or royalty fees required. We offer predictable pricing that supports faster and more accessible discovery.
Flexible Discovery
Maximize epitope coverage and de-risk your HuTg mice discovery campaign by integrating DiversimAb mice for expanded antibody diversity.
Want to learn more about Twist's antibody discovery services?
Connect with one of our experts to develop a tailored project proposal to support your specific research needs.
Applications
Find Fully Human Hits in Weeks with Single B-Cell Screening
HuTg mice, like our DiversimAb platform, generate potent, highly-specific therapeutic and reagent antibody candidates. Both platforms are fully compatible with our Beacon-based single B-cell screening, enabling high-resolution, high-throughput hit identification and sequencing in a few weeks. Twist’s HuTg mouse workflow is particularly well suited for single B-cell screening, as they generate highly productive and active B cell populations, which is not typically seen with other humanized mouse platforms.
Hybridoma-Compatible Workflow
Twist also provides an end-to-end, adaptable hybridoma workflow for HuTg and DiversimAb mice that includes hybridoma generation, high-throughput screening, and sequencing to uncover promising hits.
Applications
Find Fully Human Hits in Weeks with Single B-Cell Screening
HuTg mice, like our DiversimAb platform, generate potent, highly-specific therapeutic and reagent antibody candidates. Both platforms are fully compatible with our Beacon-based single B-cell screening, enabling high-resolution, high-throughput hit identification and sequencing in a few weeks. Twist’s HuTg mouse workflow is particularly well suited for single B-cell screening, as they generate highly productive and active B cell populations, which is not typically seen with other humanized mouse platforms.
Hybridoma-Compatible Workflow
Twist also provides an end-to-end, adaptable hybridoma workflow for HuTg and DiversimAb mice that includes hybridoma generation, high-throughput screening, and sequencing to uncover promising hits.
Resources
Learn more about humanized mice and Twist's in vivo discovery platforms
Webinar
Product Sheet
Twist Products are for research use only. Not for diagnostic use.
Case studies are not predictive of results achieved in other institutions. Results vary.
Resources
Learn more about humanized mice and Twist's in vivo discovery platforms
Twist Products are for research use only. Not for diagnostic use.
Case studies are not predictive of results achieved in other institutions. Results vary.
Get in Touch With Us
Antibody enthusiasts are standing by and would love to hear more about your discovery needs. Challenging targets welcome!
