OVERVIEW DATA ORDERING & SPECS RESOURCES
OVERVIEW

Twist’s Universal Adapter Systems improve the performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist’s Library Preparation and Target Enrichment products, the systems consist of Twist’s Universal Adapters and either Twist Unique Dual-Indexed (UDI) Primers or Twist HT UDI Primers. Alternatively, eliminate the PCR step altogether with Twist Full Length UDI Adapters.

Twist Full Length UDI Adapters

Twist's latest NGS adapter sets are full length unique dual index adapters (10 bp in length) that enable PCR-free whole genome sequencing (WGS).   

With 1,536 adapters available, users have the ability to multiplex and to maximize the efficiency of utilization of sequencing reads in a sequencing run.

The adapter sets are functional validated to ensure performance and reliability in all NGS applications. The 96-well plate configuration is designed to be automation-friendly, which can reduce hands-on-time and human error.

Twist Universal Adapters

The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step. 

Twist Universal Adapter System (10 bp)

Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.

Twist HT Universal Adapter System (12 bp)

Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries. 

Maximize Performance
Maximize Performance
Drastically reduce adapter dimer formation
Eliminate or use fewer PCR cycles during library preparation to reduce errors and bias
Increase Flexibility
Increase Flexibility
Can be used with all ‘T-A’ overhang ligation systems
Compatible with all Illumina sequencing chemistries (2- or 4-color) and flow cell types (non-patterned and patterned)
Boost Efficiency
Boost Efficiency
Scale your multiplexing with up to 3,072 available indexes
Ready-to-use formulation
Reduces per-sample sequencing cost
No More Sample Mix Up

Looking for a streamlined method to detect sample mix-ups?
See how the Twist Human Sample ID kit integrates with Twist’s Universal Adapter Systems.

Learn More

Twist’s Universal Adapter Systems improve the performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist’s Library Preparation and Target Enrichment products, the systems consist of Twist’s Universal Adapters and either Twist Unique Dual-Indexed (UDI) Primers or Twist HT UDI Primers. Alternatively, eliminate the PCR step altogether with Twist Full Length UDI Adapters.

Twist Full Length UDI Adapters

Twist's latest NGS adapter sets are full length unique dual index adapters (10 bp in length) that enable PCR-free whole genome sequencing (WGS).   

With 1,536 adapters available, users have the ability to multiplex and to maximize the efficiency of utilization of sequencing reads in a sequencing run.

The adapter sets are functional validated to ensure performance and reliability in all NGS applications. The 96-well plate configuration is designed to be automation-friendly, which can reduce hands-on-time and human error.

Twist Universal Adapters

The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step. 

Twist Universal Adapter System (10 bp)

Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.

Twist HT Universal Adapter System (12 bp)

Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries. 

No More Sample Mix Up

Looking for a streamlined method to detect sample mix-ups?
See how the Twist Human Sample ID kit integrates with Twist’s Universal Adapter Systems.

Learn More

Maximize Performance
Maximize Performance
Drastically reduce adapter dimer formation
Eliminate or use fewer PCR cycles during library preparation to reduce errors and bias
Increase Flexibility
Increase Flexibility
Can be used with all ‘T-A’ overhang ligation systems
Compatible with all Illumina sequencing chemistries (2- or 4-color) and flow cell types (non-patterned and patterned)
Boost Efficiency
Boost Efficiency
Scale your multiplexing with up to 3,072 available indexes
Ready-to-use formulation
Reduces per-sample sequencing cost
DATA
Increase Final Yields During Library Preparation

Twist Universal Adapters and HT Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.”

The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.

Alternatively, ligation using Twist Full Length UDI Adapters allows for the preparation of ready-to-sequence libraries without the need for amplification.

 NGS Reagents and Kits Universal Adapters Workflow
Increase Final Yields During Library Preparation

Twist Universal Adapters and HT Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.”

The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.

Alternatively, ligation using Twist Full Length UDI Adapters allows for the preparation of ready-to-sequence libraries without the need for amplification.

 NGS Reagents and Kits Universal Adapters Workflow
ORDERING & SPECS
Full-Length UDI Adapter Product SKUs (Individual 96-well plates)

107376

Twist Full Length UDI Adapters, Plate 1

107377

Twist Full Length UDI Adapters, Plate 2

107378

Twist Full Length UDI Adapters, Plate 3

107379

Twist Full Length UDI Adapters, Plate 4

107383

Twist Full Length UDI Adapters, Plate 5

107384

Twist Full Length UDI Adapters, Plate 6

107385

Twist Full Length UDI Adapters, Plate 7

107386

Twist Full Length UDI Adapters, Plate 8

107387

Twist Full Length UDI Adapters, Plate 9

107388

Twist Full Length UDI Adapters, Plate 10

107389

Twist Full Length UDI Adapters, Plate 11

107390

Twist Full Length UDI Adapters, Plate 12

107391

Twist Full Length UDI Adapters, Plate 13

107392

Twist Full Length UDI Adapters, Plate 14

107393

Twist Full Length UDI Adapters, Plate 15

107394

Twist Full Length UDI Adapters, Plate 16
Full-Length UDI Adapter Product SKUs (Bundled set of 4x96-well plates)

107381

Twist Full Length UDI Adapters, 384 samples- Set 1

107469

Twist Full Length UDI Adapters, 384 samples- Set 2

107470

Twist Full Length UDI Adapters, 384 samples- Set 3

107471

Twist Full Length UDI Adapters, 384 samples- Set 4
HT Universal Adapter System Product SKUs

106390

Twist HT Universal Adapter System TruSeq Comp, 96W AAA01-04

106391

Twist HT Universal Adapter System TruSeq Comp, 96W AAA05-08

106392

Twist HT Universal Adapter System TruSeq Comp, 96W AAA09-12

106393

Twist HT Universal Adapter System TruSeq Comp, 96W AAA13-16

106396

Twist HT Universal Adapter System TruSeq Comp, 96W BBB01-04 (Coming soon!)

106398

Twist HT Universal Adapter System TruSeq Comp, 96W BBB05-08 (Coming soon!)

106399

Twist HT Universal Adapter System TruSeq Comp, 96W BBB09-12 (Coming soon!)

106400

Twist HT Universal Adapter System TruSeq Comp, 96W BBB13-16 (Coming soon!)

106401

Twist HT Universal Adapter System TruSeq Comp, 384W AAA01

106402

Twist HT Universal Adapter System TruSeq Comp, 384W AAA02

106403

Twist HT Universal Adapter System TruSeq Comp, 384W AAA03

106404

Twist HT Universal Adapter System TruSeq Comp, 384W AAA04

106405

Twist HT Universal Adapter System TruSeq Comp, 384W BBB01 (Coming soon!)

106406

Twist HT Universal Adapter System TruSeq Comp, 384W BBB02 (Coming soon!)

106407

Twist HT Universal Adapter System TruSeq Comp, 384W BBB03 (Coming soon!)

106408

Twist HT Universal Adapter System TruSeq Comp, 384W BBB04 (Coming soon!)
Universal Adapter System Product SKUs

101307

Twist Universal Adapter System TruSeq Compatible, 16 Samples

101308

Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate A

101309

Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate B

101310

Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate C

101311

Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate D

The Twist Universal Adapter System is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

Full-Length UDI Adapter Product SKUs (Individual 96-well plates)

107376

Twist Full Length UDI Adapters, Plate 1

107377

Twist Full Length UDI Adapters, Plate 2

107378

Twist Full Length UDI Adapters, Plate 3

107379

Twist Full Length UDI Adapters, Plate 4

107383

Twist Full Length UDI Adapters, Plate 5

107384

Twist Full Length UDI Adapters, Plate 6

107385

Twist Full Length UDI Adapters, Plate 7

107386

Twist Full Length UDI Adapters, Plate 8

107387

Twist Full Length UDI Adapters, Plate 9

107388

Twist Full Length UDI Adapters, Plate 10

107389

Twist Full Length UDI Adapters, Plate 11

107390

Twist Full Length UDI Adapters, Plate 12

107391

Twist Full Length UDI Adapters, Plate 13

107392

Twist Full Length UDI Adapters, Plate 14

107393

Twist Full Length UDI Adapters, Plate 15

107394

Twist Full Length UDI Adapters, Plate 16
Full-Length UDI Adapter Product SKUs (Bundled set of 4x96-well plates)

107381

Twist Full Length UDI Adapters, 384 samples- Set 1

107469

Twist Full Length UDI Adapters, 384 samples- Set 2

107470

Twist Full Length UDI Adapters, 384 samples- Set 3

107471

Twist Full Length UDI Adapters, 384 samples- Set 4
HT Universal Adapter System Product SKUs

106390

Twist HT Universal Adapter System TruSeq Comp, 96W AAA01-04

106391

Twist HT Universal Adapter System TruSeq Comp, 96W AAA05-08

106392

Twist HT Universal Adapter System TruSeq Comp, 96W AAA09-12

106393

Twist HT Universal Adapter System TruSeq Comp, 96W AAA13-16

106396

Twist HT Universal Adapter System TruSeq Comp, 96W BBB01-04 (Coming soon!)

106398

Twist HT Universal Adapter System TruSeq Comp, 96W BBB05-08 (Coming soon!)

106399

Twist HT Universal Adapter System TruSeq Comp, 96W BBB09-12 (Coming soon!)

106400

Twist HT Universal Adapter System TruSeq Comp, 96W BBB13-16 (Coming soon!)

106401

Twist HT Universal Adapter System TruSeq Comp, 384W AAA01

106402

Twist HT Universal Adapter System TruSeq Comp, 384W AAA02

106403

Twist HT Universal Adapter System TruSeq Comp, 384W AAA03

106404

Twist HT Universal Adapter System TruSeq Comp, 384W AAA04

106405

Twist HT Universal Adapter System TruSeq Comp, 384W BBB01 (Coming soon!)

106406

Twist HT Universal Adapter System TruSeq Comp, 384W BBB02 (Coming soon!)

106407

Twist HT Universal Adapter System TruSeq Comp, 384W BBB03 (Coming soon!)

106408

Twist HT Universal Adapter System TruSeq Comp, 384W BBB04 (Coming soon!)
Universal Adapter System Product SKUs

101307

Twist Universal Adapter System TruSeq Compatible, 16 Samples

101308

Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate A

101309

Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate B

101310

Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate C

101311

Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate D

The Twist Universal Adapter System is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.

RESOURCES
Increase multiplexing and library yield
Increase multiplexing and library yield
Increase multiplexing and library yield